Restriction map for
pFN21AE3971
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII Cfr10I CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HgaI HphI
Ksp632I KspI MluI MluNI MroI
MscI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 430 GGTACC G^GTACC
AccI 545 GTAGAC GT^[AC][GT]AC
AcsI 1066 GAATTT [GA]^AATT[CT]
AcyI 49 GGCGCC G[GA]^CG[CT]C
AflII 850 CTTAAG C^TTAAG
AlwNI 1250 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1005 CCTAAGG CC^T[ACGT]AGG
ApoI 1066 GAATTT [GA]^AATT[CT]
Asp718I 430 GGTACC G^GTACC
AspHI 219 GAGCTC G[AT]GC[AT]^C
BbeI 49 GGCGCC GGCGC^C
BciVI 82 GTATCC GTATCC(6/5)
BcnI 934 CCGGG CC^[GC]GG
BfrI 850 CTTAAG C^TTAAG
BglI 41 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 139 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsaI 1274 GAGACC GGTCTC(1/5)
BsaWI 92 TCCGGT [AT]^CCGG[AT]
BsiHKAI 219 GAGCTC G[AT]GC[AT]^C
BspDI 163 ATCGAT AT^CGAT
BstEII 1180 GGTAACC G^GT[ACGT]ACC
BstPI 1180 GGTAACC G^GT[ACGT]ACC
Bsu36I 1005 CCTAAGG CC^T[ACGT]AGG
CfoI 50 GCGC GCG^C
ClaI 163 ATCGAT AT^CGAT
DsaI 62 CCGTGG C^C[GA][CT]GG
Eco81I 1005 CCTAAGG CC^T[ACGT]AGG
EcoNI 846 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1180 GGTAACC G^GT[ACGT]ACC
HaeII 49 GGCGCC [GA]GCGC^[CT]
HhaI 50 GCGC GCG^C
Hin1I 49 GGCGCC G[GA]^CG[CT]C
HinP1I 50 GCGC G^CGC
HpaI 1000 GTTAAC GTT^AAC
KasI 49 GGCGCC G^GCGCC
KpnI 430 GGTACC GGTAC^C
MamI 139 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 640 CAATTG C^AATTG
MslI 1264 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 640 CAATTG C^AATTG
NarI 49 GGCGCC GG^CGCC
NciI 934 CCGGG CC^[GC]GG
PflMI 273 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 424 GACTC GAGTC(4/5)
PpuMI 663 GGGACCT [GA]G^G[AT]CC[CT]
SacI 219 GAGCTC GAGCT^C
ScaI 599 AGTACT AGT^ACT
SfoI 49 GGCGCC GGC^GCC
StuI 867 AGGCCT AGG^CCT
Tsp45I 719 GTCAC ^GT[GC]AC
TspRI 1284 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 273 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 39 GGGCCC GGGCC^C
68 GGGCCC
Asp700I 114 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
535 GAAGCCCTTC
BanI 49 GGCGCC G^G[CT][GA]CC
430 GGTACC
BclI 360 TGATCA T^GATCA
792 TGATCA
BseRI 844 CTCCTC GAGGAG(10/8)
1055 GAGGAG
BsmFI 663 GGGAC GGGAC(10/14)
1010 GGGAC
BspHI 672 TCATGA T^CATGA
861 TCATGA
BspMI 666 ACCTGC ACCTGC(4/8)
942 ACCTGC
BstXI 596 CCAAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
816 CCAAGGTGTTGG
EcoT14I 816 CCAAGG C^C[AT][AT]GG
870 CCTTGG
FauI 7 GCGGG CCCGC(4/6)
77 GCGGG
FbaI 360 TGATCA T^GATCA
792 TGATCA
HincII 991 GTCAAC GT[CT]^[GA]AC
1000 GTTAAC
HindII 991 GTCAAC GT[CT]^[GA]AC
1000 GTTAAC
HindIII 128 AAGCTT A^AGCTT
679 AAGCTT
MspA1I 373 CAGCTG C[AC]G^C[GT]G
388 CAGCTG
PstI 391 CTGCAG CTGCA^G
741 CTGCAG
PvuII 373 CAGCTG CAG^CTG
388 CAGCTG
RcaI 672 TCATGA T^CATGA
861 TCATGA
SfcI 391 CTGCAG C^T[GA][CT]AG
741 CTGCAG
SmlI 460 CTTGAG C^T[CT][GA]AG
850 CTTAAG
StyI 816 CCAAGG C^C[AT][AT]GG
870 CCTTGG
TaqI 122 TCGA T^CGA
164 TCGA
TfiI 799 GATTC G^A[AT]TC
1099 GATTC
TthHB8I 122 TCGA T^CGA
164 TCGA
XmnI 114 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
535 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I XhoII