(Circular) (Six-base) MAPSORT of: pFN21AE3971.seq Check: 1963 from: 1 to: 6067 pFN21AE3971 6067 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2551 3410 1937 Size: 614 859 4594 Fragments arranged by size: 4594 859 614 Acc65I G'GTAC_C Cuts at: 2435 3388 2435 Size: 953 5114 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 2595 3117 4130 5514 5782 1632 Size: 900 63 522 1013 1384 268 1917 Fragments arranged by size: 1917 1384 1013 900 522 268 63 AflII C'TTAA_G Cuts at: 820 1017 2855 820 Size: 197 1838 4032 Fragments arranged by size: 4032 1838 197 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2260 2421 1184 Size: 1076 161 4830 Fragments arranged by size: 4830 1076 161 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2108 2140 2108 Size: 32 6035 AlwNI CAG_nnn'CTG Cuts at: 3260 3810 5382 3260 Size: 550 1572 3945 Fragments arranged by size: 3945 1572 550 ApaI G_GGCC'C Cuts at: 2048 2077 2048 Size: 29 6038 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 3071 3376 3602 3754 5863 1493 Size: 1578 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 1578 305 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 3392 1870 Size: 73 1449 4545 Fragments arranged by size: 4545 1449 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2419 2452 2419 Size: 33 6034 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2435 3388 4843 618 Size: 325 217 627 267 381 953 1455 1842 Fragments arranged by size: 1842 1455 953 627 381 325 267 217 BanII G_rGCy'C Cuts at: 1391 2048 2077 2228 3386 1391 Size: 657 29 151 1158 4072 Fragments arranged by size: 4072 1158 657 151 29 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 6067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2098 3959 5169 2098 Size: 1861 1210 2996 Fragments arranged by size: 2996 1861 1210 BclI T'GATC_A Cuts at: 1548 2365 2797 1548 Size: 817 432 4818 Fragments arranged by size: 4818 817 432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2052 4678 Size: 107 122 71 631 605 337 43 2626 Cuts at: 4678 136 Size: 1525 Fragments arranged by size: 2626 1525 631 605 337 122 107 71 43 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2048 2077 3290 4128 5284 1790 Size: 258 29 1213 838 1156 2573 Fragments arranged by size: 2573 1213 1156 838 258 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2251 3798 4756 Size: 416 53 83 399 24 834 1547 958 Cuts at: 4756 442 Size: 1753 Fragments arranged by size: 1753 1547 958 834 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2135 2306 2552 4727 1239 Size: 887 9 171 246 2175 2579 Fragments arranged by size: 2579 2175 887 246 171 9 Bpu10I CC'TnA_GC Cuts at: 2222 2222 Size: 6067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 4192 5057 5355 2486 Size: 1706 865 298 3198 Fragments arranged by size: 3198 1706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3273 4730 882 Size: 617 1774 1457 2219 Fragments arranged by size: 2219 1774 1457 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 2148 6061 2148 Size: 3913 2154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3874 4256 4891 Size: 53 83 186 936 522 1819 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1819 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2097 4500 4898 5172 5319 5730 2097 Size: 2403 398 274 147 411 2434 Fragments arranged by size: 2434 2403 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 2279 2309 1178 Size: 30 165 30 705 30 141 30 4936 Fragments arranged by size: 4936 705 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2840 3075 1186 Size: 1654 235 4178 Fragments arranged by size: 4178 1654 235 BseYI C'CCAG_C Cuts at: 2026 5270 5766 2026 Size: 3244 496 2327 Fragments arranged by size: 3244 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2954 3323 1163 Size: 573 630 588 369 3907 Fragments arranged by size: 3907 630 588 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2228 4128 4213 5284 2228 Size: 1900 85 1071 3011 Fragments arranged by size: 3011 1900 1071 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2077 2228 3290 3386 4128 4213 Size: 399 258 29 151 1062 96 742 85 Cuts at: 4213 5284 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1062 742 399 258 151 96 85 29 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 2677 2866 3956 5686 2677 Size: 189 1090 1730 3058 Fragments arranged by size: 3058 1730 1090 189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2680 2956 3422 844 Size: 1836 276 466 3489 Fragments arranged by size: 3489 1836 466 276 BsrBI CCG'CTC Cuts at: 1394 1823 3954 1394 Size: 429 2131 3507 Fragments arranged by size: 3507 2131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 3185 1184 Size: 2001 4066 BstXI CCAn_nnnn'nTGG Cuts at: 2608 2828 2608 Size: 220 5847 BstYI r'GATC_y Cuts at: 1972 2149 2302 2931 3397 3764 4149 4166 5618 Size: 177 153 629 466 367 385 17 1452 Cuts at: 5618 5704 5716 6062 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 629 466 385 367 346 177 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3011 3011 Size: 6067 BtgI C'CryG_G Cuts at: 513 1065 2067 513 Size: 552 1002 4513 Fragments arranged by size: 4513 1002 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3288 3583 4377 4397 3288 Size: 295 794 20 4958 Fragments arranged by size: 4958 794 295 20 ClaI AT'CG_AT Cuts at: 1554 2169 1554 Size: 615 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032 Size: 54 1425 50 275 18 1684 889 1629 Cuts at: 6032 8 Size: 43 Fragments arranged by size: 1684 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3997 1353 Size: 2644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2226 2226 Size: 6067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2135 2306 2532 2552 2595 3117 Size: 393 494 9 171 226 20 43 522 Cuts at: 3117 4130 4727 5514 5782 1239 Size: 1013 597 787 268 1524 Fragments arranged by size: 1524 1013 787 597 522 494 393 268 226 171 43 20 9 EcoNI CCTnn'n_nnAGG Cuts at: 2855 2855 Size: 6067 EcoO109I rG'GnC_Cy Cuts at: 2044 2073 2074 2669 2044 Size: 29 1 595 5442 Fragments arranged by size: 5442 595 29 1 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2519 2519 Size: 6067 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2058 5214 1678 Size: 380 3156 2531 Fragments arranged by size: 3156 2531 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 3130 3162 4708 Size: 32 135 32 722 32 804 32 1546 Cuts at: 4708 4740 4782 4814 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1546 804 722 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2998 3007 3411 3657 669 Size: 988 281 1060 9 404 246 3079 Fragments arranged by size: 3079 1060 988 404 281 246 9 HindIII A'AGCT_T Cuts at: 748 2133 2684 748 Size: 1385 551 4131 Fragments arranged by size: 4131 1385 551 HpaI GTT'AAC Cuts at: 3007 3657 3007 Size: 650 5417 KasI G'GCGC_C Cuts at: 2054 2054 Size: 6067 KpnI G_GTAC'C Cuts at: 2439 3392 2439 Size: 953 5114 MfeI C'AATT_G Cuts at: 2645 3666 2645 Size: 1021 5046 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2659 5181 5365 1305 Size: 1354 2522 184 2007 Fragments arranged by size: 2522 2007 1354 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3273 4025 4384 4543 518 Size: 648 2107 752 359 159 2042 Fragments arranged by size: 2107 2042 752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2380 2395 3483 4160 5308 5553 1606 Size: 774 15 1088 677 1148 245 2120 Fragments arranged by size: 2120 1148 1088 774 677 245 15 NarI GG'CG_CC Cuts at: 2055 2055 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5515 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 2048 1348 Size: 700 5367 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 1108 Size: 1176 4891 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2140 4136 4168 5649 5681 2108 Size: 32 1996 32 1481 32 2494 Fragments arranged by size: 2494 1996 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2669 2669 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspOMI G'GGCC_C Cuts at: 2044 2073 2044 Size: 29 6038 PstI C_TGCA'G Cuts at: 830 2400 2750 3419 830 Size: 1570 350 669 3478 Fragments arranged by size: 3478 1570 669 350 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2380 2395 2380 Size: 15 6052 SacI G_AGCT'C Cuts at: 2228 2228 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 2606 4315 6027 2606 Size: 1709 1712 2646 Fragments arranged by size: 2646 1712 1709 SfcI C'TryA_G Cuts at: 826 1045 2396 2746 3415 4550 5231 5422 826 Size: 219 1351 350 669 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 681 669 350 219 191 SfoI GGC'GCC Cuts at: 2056 2056 Size: 6067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 2855 4171 5072 5334 820 Size: 197 926 522 390 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 522 390 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 2874 1517 Size: 1357 4710 StyI C'CwwG_G Cuts at: 513 1065 2821 2875 3506 513 Size: 552 1756 54 631 3074 Fragments arranged by size: 3074 1756 631 552 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 4111 4264 4449 4894 325 Size: 1255 2514 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2359 2604 4313 Size: 274 80 33 51 1443 382 245 1709 Cuts at: 4313 6025 96 Size: 1712 138 Fragments arranged by size: 1712 1709 1443 382 274 245 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2123 2544 4196 2123 Size: 421 1652 3994 Fragments arranged by size: 3994 1652 421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI