Restriction map for
pFN21AB4957
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BcnI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRV FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MluI MluNI
MroI MscI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspBI StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 307 CTTAAG C^TTAAG
AocI 570 CCTAAGG CC^T[ACGT]AGG
AvaII 303 GGTCC G^G[AT]CC
BbsI 1574 GAAGAC GAAGAC(2/6)
BclI 801 TGATCA T^GATCA
BfrI 307 CTTAAG C^TTAAG
BpuAI 1574 GAAGAC GAAGAC(2/6)
BsaBI 370 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 656 GAGACC GGTCTC(1/5)
BseRI 1388 GAGGAG GAGGAG(10/8)
BsmI 250 GCATTC GAATGC(1/-1)
BspMI 845 ACCTGC ACCTGC(4/8)
BstBI 1571 TTCGAA TT^CGAA
BstXI 1551 CCATGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 570 CCTAAGG CC^T[ACGT]AGG
DsaI 879 CCGTGG C^C[GA][CT]GG
EarI 145 GAAGAG CTCTTC(1/4)
Eco81I 570 CCTAAGG CC^T[ACGT]AGG
EcoNI 660 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 26 GAATTC G^AATTC
EcoT14I 1072 CCAAGG C^C[AT][AT]GG
EcoT22I 248 ATGCAT ATGCA^T
FbaI 801 TGATCA T^GATCA
HindIII 688 AAGCTT A^AGCTT
HinfI 297 GAATC G^A[ACGT]TC
HphI 114 GGTGA GGTGA(8/7)
Ksp632I 145 GAAGAG CTCTTC(1/4)
MamI 370 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 71 CAATTG C^AATTG
MslI 1552 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 71 CAATTG C^AATTG
NsiI 248 ATGCAT ATGCA^T
NspI 1497 GCATGC [GA]CATG^[CT]
NspV 1571 TTCGAA TT^CGAA
PstI 1382 CTGCAG CTGCA^G
ScaI 356 AGTACT AGT^ACT
SfcI 1382 CTGCAG C^T[GA][CT]AG
SfuI 1571 TTCGAA TT^CGAA
SmlI 307 CTTAAG C^T[CT][GA]AG
SpeI 199 ACTAGT A^CTAGT
SphI 1497 GCATGC GCATG^C
SspI 737 AATATT AAT^ATT
StyI 1072 CCAAGG C^C[AT][AT]GG
TfiI 297 GAATC G^A[AT]TC
Tsp45I 115 GTGAC ^GT[GC]AC
XbaI 621 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 930 CCGC CCGC(-3/-1)
640 GCGG
BbvCI 490 GCTGAGG CCTCAGC(-5/-2)
1077 GCTGAGG
BbvI 388 GCAGC GCAGC(8/12)
1501 GCAGC
BglII 108 AGATCT A^GATCT
1206 AGATCT
BpmI 409 CTCCAG CTGGAG(16/14)
971 CTCCAG
BsmBI 406 CGTCTC CGTCTC(1/5)
1120 GAGACG
BsmFI 995 GGGAC GGGAC(10/14)
1333 GGGAC
Cac8I 1083 GCGAGC GC[ACGT]^[ACGT]GC
1497 GCATGC
Cfr13I 303 GGTCC G^G[ACGT]CC
1427 GGCCC
HaeIII 519 GGCC GG^CC
1427 GGCC
MwoI 388 GCAGCCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1384 GCAGGAGGAGC
PflMI 39 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1538 CCAATAAATGG
Sau96I 303 GGTCC G^G[ACGT]CC
1427 GGCCC
TaqI 1185 TCGA T^CGA
1572 TCGA
TseI 388 GCAGC G^C[AT]GC
1501 GCAGC
TthHB8I 1185 TCGA T^CGA
1572 TCGA
Van91I 39 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1538 CCAATAAATGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AseI AsnI BciVI BfaI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstYI DdeI DpnII
EcoRII Fnu4HI FokI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV PshBI RsaI Sau3AI ScrFI
SfaNI Tru9I Tsp509I TspRI XhoII