(Circular) (Six-base) MAPSORT of: pFN21AB4957.seq Check: 7816 from: 1 to: 6307 pFN21AB4957 6307 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 1937 Size: 1713 4594 Acc65I G'GTAC_C Cuts at: 3628 3628 Size: 6307 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2775 3432 4370 5754 6022 1632 Size: 1143 657 938 1384 268 1917 Fragments arranged by size: 1917 1384 1143 938 657 268 AflII C'TTAA_G Cuts at: 820 1017 2312 820 Size: 197 1295 4815 Fragments arranged by size: 4815 1295 197 AflIII A'CryG_T Cuts at: 1203 5206 6183 1203 Size: 4003 977 1327 Fragments arranged by size: 4003 1327 977 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6307 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6307 AlwNI CAG_nnn'CTG Cuts at: 4050 5622 4050 Size: 1572 4735 ApaLI G'TGCA_C Cuts at: 4364 5520 4364 Size: 1156 5151 ApoI r'AATT_y Cuts at: 1493 2031 2963 3572 3616 3842 3994 6103 1493 Size: 538 932 609 44 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 609 538 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 2021 2264 3195 4861 160 Size: 872 989 243 931 1666 1606 Fragments arranged by size: 1666 1606 989 931 872 243 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 3632 1870 Size: 73 1689 4545 Fragments arranged by size: 4545 1689 73 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3628 5083 618 Size: 325 217 627 1841 1455 1842 Fragments arranged by size: 1842 1841 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3626 1391 Size: 2235 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3586 928 Size: 971 1687 3649 Fragments arranged by size: 3649 1687 971 BbvCI CC'TCA_GC Cuts at: 2496 3083 2496 Size: 587 5720 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 1903 Size: 34 2561 34 3678 Fragments arranged by size: 3678 2561 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2413 2428 3550 4199 5409 2413 Size: 15 1122 649 1210 3311 Fragments arranged by size: 3311 1210 1122 649 15 BclI T'GATC_A Cuts at: 1548 2806 1548 Size: 1258 5049 BfrBI ATG'CAT Cuts at: 2255 2255 Size: 6307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4918 136 Size: 107 122 71 631 605 3246 1525 Fragments arranged by size: 3246 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2113 3211 6302 2113 Size: 1098 3091 2118 Fragments arranged by size: 3091 2118 1098 BlpI GC'TnA_GC Cuts at: 3724 3724 Size: 6307 Bme1580I G_kGCm'C Cuts at: 1790 4368 5524 1790 Size: 2578 1156 2573 Fragments arranged by size: 2578 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2038 4038 4996 Size: 416 53 83 399 24 621 2000 958 Cuts at: 4996 442 Size: 1753 Fragments arranged by size: 2000 1753 958 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 2961 4967 1239 Size: 1160 562 2006 2579 Fragments arranged by size: 2579 2006 1160 562 Bpu10I CC'TnA_GC Cuts at: 2496 3083 2496 Size: 587 5720 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4432 5297 5595 4432 Size: 865 298 5144 Fragments arranged by size: 5144 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2655 4970 882 Size: 617 1156 2315 2219 Fragments arranged by size: 2315 2219 1156 617 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 2379 6301 2379 Size: 3922 2385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4114 4496 5131 275 Size: 53 83 186 936 2581 382 635 1451 Fragments arranged by size: 2581 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4740 5138 5412 5559 5970 4740 Size: 398 274 147 411 5077 Fragments arranged by size: 5077 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3387 3411 3417 3441 1178 Size: 30 165 30 1984 24 6 24 4044 Fragments arranged by size: 4044 1984 165 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3408 1186 Size: 2222 4085 BseYI C'CCAG_C Cuts at: 3334 5510 6006 3334 Size: 2176 496 3635 Fragments arranged by size: 3635 2176 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5734 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4518 5546 1540 Size: 275 186 1756 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4368 4453 5524 4368 Size: 85 1071 5151 Fragments arranged by size: 5151 1071 85 BsmI GAATG_Cn' Cuts at: 2255 3818 3911 2255 Size: 1563 93 4651 Fragments arranged by size: 4651 1563 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2417 3119 2417 Size: 702 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3626 4368 4453 5524 1391 Size: 399 1836 742 85 1071 2174 Fragments arranged by size: 2174 1836 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 4196 5926 4196 Size: 1730 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2859 3662 844 Size: 2015 803 3489 Fragments arranged by size: 3489 2015 803 BsrBI CCG'CTC Cuts at: 1394 1823 4194 1394 Size: 429 2371 3507 Fragments arranged by size: 3507 2371 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4802 4976 65 Size: 1183 3554 174 1396 Fragments arranged by size: 3554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4951 5138 1700 Size: 110 3141 187 2869 Fragments arranged by size: 3141 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6307 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 4367 5379 4367 Size: 1012 5295 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6307 BstBI TT'CG_AA Cuts at: 3577 5111 3577 Size: 1534 4773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6307 BstXI CCAn_nnnn'nTGG Cuts at: 3563 3563 Size: 6307 BstYI r'GATC_y Cuts at: 1972 2113 2221 2707 3211 3637 4004 4389 4406 Size: 141 108 486 504 426 367 385 17 Cuts at: 4406 5858 5944 5956 6302 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 504 486 426 385 367 346 141 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2576 2576 Size: 6307 BtgI C'CryG_G Cuts at: 513 1065 2884 513 Size: 552 1819 3936 Fragments arranged by size: 3936 1819 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5568 Fragments arranged by size: 5568 683 56 BtsI GCAGTG_nn' Cuts at: 3823 4617 4637 3823 Size: 794 20 5493 Fragments arranged by size: 5493 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5314 809 Size: 507 3998 1802 Fragments arranged by size: 3998 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3754 4643 6272 Size: 54 1425 50 275 18 1924 889 1629 Cuts at: 6272 8 Size: 43 Fragments arranged by size: 1924 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3754 1537 Size: 275 1942 4090 Fragments arranged by size: 4090 1942 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 4237 1353 Size: 792 2092 3423 Fragments arranged by size: 3423 2092 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4894 5268 5414 387 Size: 1064 138 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2775 2961 3432 4370 4967 5754 Size: 393 767 376 186 471 938 597 787 Cuts at: 5754 6022 1239 Size: 268 1524 Fragments arranged by size: 1524 938 787 767 597 471 393 376 268 186 EcoNI CCTnn'n_nnAGG Cuts at: 2669 2669 Size: 6307 EcoRI G'AATT_C Cuts at: 2031 3616 3994 2031 Size: 1585 378 4344 Fragments arranged by size: 4344 1585 378 FspI TGC'GCA Cuts at: 4813 4813 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 5454 1678 Size: 3776 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2206 2238 2432 2464 2603 Size: 32 135 32 634 32 194 32 139 Cuts at: 2603 2635 2786 2818 4948 4980 5022 5054 1373 Size: 32 151 32 2130 32 42 32 2626 Fragments arranged by size: 2626 2130 634 194 151 139 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3651 3897 669 Size: 988 281 1713 246 3079 Fragments arranged by size: 3079 1713 988 281 246 HindIII A'AGCT_T Cuts at: 748 2693 748 Size: 1945 4362 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KpnI G_GTAC'C Cuts at: 3632 3632 Size: 6307 MfeI C'AATT_G Cuts at: 2076 3906 2076 Size: 1830 4477 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 3250 3397 5421 5605 1305 Size: 972 973 147 2024 184 2007 Fragments arranged by size: 2024 2007 973 972 184 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4485 Fragments arranged by size: 4485 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3561 4265 4624 4783 518 Size: 648 2395 704 359 159 2042 Fragments arranged by size: 2395 2042 704 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3723 4400 5548 5793 1606 Size: 2117 677 1148 245 2120 Fragments arranged by size: 2120 2117 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5755 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NsiI A_TGCA'T Cuts at: 2257 2257 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 3506 3665 5210 1132 Size: 97 2277 159 1545 2229 Fragments arranged by size: 2277 2229 1545 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6307 PciI A'CATG_T Cuts at: 5206 5206 Size: 6307 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2050 3549 1108 Size: 902 40 1499 3866 Fragments arranged by size: 3866 1499 902 40 PfoI T'CCnGG_A Cuts at: 2415 2464 2415 Size: 49 6258 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PstI C_TGCA'G Cuts at: 830 3391 3659 830 Size: 2561 268 3478 Fragments arranged by size: 3478 2561 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 2363 4555 6267 2363 Size: 2192 1712 2403 Fragments arranged by size: 2403 2192 1712 SfcI C'TryA_G Cuts at: 826 1045 3387 3655 4790 5471 5662 826 Size: 219 2342 268 1135 681 191 1471 Fragments arranged by size: 2342 1471 1135 681 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6307 SmaI CCC'GGG Cuts at: 1872 3634 1872 Size: 1762 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 4411 5312 5574 820 Size: 197 926 369 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 369 262 197 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 2204 152 Size: 2052 4255 SphI G_CATG'C Cuts at: 1132 3506 3665 1132 Size: 2374 159 3774 Fragments arranged by size: 3774 2374 159 SspI AAT'ATT Cuts at: 5 52 2744 4231 5 Size: 47 2692 1487 2081 Fragments arranged by size: 2692 2081 1487 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6307 StyI C'CwwG_G Cuts at: 513 1065 3077 3746 513 Size: 552 2012 669 3074 Fragments arranged by size: 3074 2012 669 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4334 4351 4504 4689 5134 325 Size: 1255 2754 17 153 185 445 1498 Fragments arranged by size: 2754 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2207 2361 3494 Size: 274 80 33 51 1443 230 154 1133 Cuts at: 3494 4553 6265 96 Size: 1059 1712 138 Fragments arranged by size: 1712 1443 1133 1059 274 230 154 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3391 3423 6267 6299 3391 Size: 32 2844 32 3399 Fragments arranged by size: 3399 2844 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6307 XbaI T'CTAG_A Cuts at: 2626 3643 2626 Size: 1017 5290 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 3632 1870 Size: 1762 4545 XmnI GAAnn'nnTTC Cuts at: 4436 4436 Size: 6307 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI