Restriction map for
pFN21AB4957
(Linear) (Six-base) MAPSORT of: KIBB4957.seq Check: 2720 from: 1 to: 1602
KIBB4957.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1427 1602
Size: 770 657 175
Fragments arranged by size:
770 657 175
AflII C'TTAA_G
Cuts at: 0 307 1602
Size: 307 1295
ApoI r'AATT_y
Cuts at: 0 26 958 1567 1602
Size: 26 932 609 35
Fragments arranged by size:
932 609 35 26
AseI AT'TA_AT
Cuts at: 0 16 259 1190 1602
Size: 16 243 931 412
Fragments arranged by size:
931 412 243 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1581 1602
Size: 1581 21
BbvCI CC'TCA_GC
Cuts at: 0 491 1078 1602
Size: 491 587 524
Fragments arranged by size:
587 524 491
BciVI GTATCCnnnnn_n'
Cuts at: 0 408 423 1545 1602
Size: 408 15 1122 57
Fragments arranged by size:
1122 408 57 15
BclI T'GATC_A
Cuts at: 0 801 1602
Size: 801 801
BfrBI ATG'CAT
Cuts at: 0 250 1602
Size: 250 1352
BglII A'GATC_T
Cuts at: 0 108 1206 1602
Size: 108 1098 396
Fragments arranged by size:
1098 396 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 1602
Size: 33 1569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 956 1602
Size: 394 562 646
Fragments arranged by size:
646 562 394
Bpu10I CC'TnA_GC
Cuts at: 0 491 1078 1602
Size: 491 587 524
Fragments arranged by size:
587 524 491
BsaI GGTCTCn'nnnn_
Cuts at: 0 650 1602
Size: 650 952
BsaBI GATnn'nnATC
Cuts at: 0 374 1602
Size: 374 1228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1382 1406 1412 1436 1602
Size: 1382 24 6 24 166
Fragments arranged by size:
1382 166 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1403 1602
Size: 1403 199
BseYI C'CCAG_C
Cuts at: 0 1329 1602
Size: 1329 273
BsmI GAATG_Cn'
Cuts at: 0 250 1602
Size: 250 1352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 412 1114 1602
Size: 412 702 488
Fragments arranged by size:
702 488 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 854 1602
Size: 854 748
BstBI TT'CG_AA
Cuts at: 0 1572 1602
Size: 1572 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1558 1602
Size: 1558 44
BstYI r'GATC_y
Cuts at: 0 108 216 702 1206 1602
Size: 108 108 486 504 396
Fragments arranged by size:
504 486 396 108 108
Bsu36I CC'TnA_GG
Cuts at: 0 571 1602
Size: 571 1031
BtgI C'CryG_G
Cuts at: 0 879 1602
Size: 879 723
EarI CTCTTCn'nnn_
Cuts at: 0 140 1602
Size: 140 1462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 770 956 1427 1602
Size: 394 376 186 471 175
Fragments arranged by size:
471 394 376 186 175
EcoNI CCTnn'n_nnAGG
Cuts at: 0 664 1602
Size: 664 938
EcoRI G'AATT_C
Cuts at: 0 26 1602
Size: 26 1576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 427 459 598 630 781 813
Size: 201 32 194 32 139 32 151 32
Cuts at: 813 1602
Size: 789
Fragments arranged by size:
789 201 194 151 139 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 688 1602
Size: 688 914
MfeI C'AATT_G
Cuts at: 0 71 1602
Size: 71 1531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1245 1392 1602
Size: 272 973 147 210
Fragments arranged by size:
973 272 210 147
MslI CAynn'nnrTG
Cuts at: 0 1556 1602
Size: 1556 46
NsiI A_TGCA'T
Cuts at: 0 252 1602
Size: 252 1350
NspI r_CATG'y
Cuts at: 0 1501 1602
Size: 1501 101
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 1544 1602
Size: 45 1499 58
Fragments arranged by size:
1499 58 45
PfoI T'CCnGG_A
Cuts at: 0 410 459 1602
Size: 410 49 1143
Fragments arranged by size:
1143 410 49
PstI C_TGCA'G
Cuts at: 0 1386 1602
Size: 1386 216
ScaI AGT'ACT
Cuts at: 0 358 1602
Size: 358 1244
SfcI C'TryA_G
Cuts at: 0 1382 1602
Size: 1382 220
SmlI C'TyrA_G
Cuts at: 0 307 1602
Size: 307 1295
SpeI A'CTAG_T
Cuts at: 0 199 1602
Size: 199 1403
SphI G_CATG'C
Cuts at: 0 1501 1602
Size: 1501 101
SspI AAT'ATT
Cuts at: 0 739 1602
Size: 739 863
StyI C'CwwG_G
Cuts at: 0 1072 1602
Size: 1072 530
TatI w'GTAC_w
Cuts at: 0 202 356 1489 1602
Size: 202 154 1133 113
Fragments arranged by size:
1133 202 154 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1386 1418 1602
Size: 1386 32 184
Fragments arranged by size:
1386 184 32
XbaI T'CTAG_A
Cuts at: 0 621 1602
Size: 621 981
Enzymes that do cut:
AcuI AflII ApoI AseI BbsI BbvCI BciVI BclI
BfrBI BglII BmrI BpmI Bpu10I BsaI BsaBI BsaXI
BseRI BseYI BsmI BsmBI BspMI BstBI BstXI BstYI
Bsu36I BtgI EarI Eco57MI EcoNI EcoRI Hin4I HindIII
MfeI MmeI MslI NsiI NspI PflMI PfoI PstI
ScaI SfcI SmlI SpeI SphI SspI StyI TatI
TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BcgI BglI BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaAI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MluI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI StuI SwaI TaqII Tth111I XcmI XhoI XmaI
XmnI ZraI