(Circular) (Six-base) MAPSORT of: pFN21AB4945.seq Check: 9021 from: 1 to: 5641 pFN21AB4945 5641 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 2786 2984 1937 Size: 472 377 198 4594 Fragments arranged by size: 4594 472 377 198 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3704 5088 5356 1632 Size: 695 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 695 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2334 4540 5517 1203 Size: 1131 2206 977 1327 Fragments arranged by size: 2206 1327 1131 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2255 2287 2255 Size: 32 5609 AlwNI CAG_nnn'CTG Cuts at: 2305 3384 4956 2305 Size: 1079 1572 2990 Fragments arranged by size: 2990 1572 1079 ApaI G_GGCC'C Cuts at: 2543 2543 Size: 5641 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2176 2950 3176 3328 5437 1493 Size: 683 774 226 152 2109 1697 Fragments arranged by size: 2109 1697 774 683 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2425 2962 4417 618 Size: 325 217 627 638 537 1455 1842 Fragments arranged by size: 1842 1455 638 627 537 325 217 BanII G_rGCy'C Cuts at: 1391 2190 2543 2960 1391 Size: 799 353 417 4072 Fragments arranged by size: 4072 799 417 353 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2783 928 Size: 971 884 3786 Fragments arranged by size: 3786 971 884 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2543 2702 3702 4858 1790 Size: 753 159 1000 1156 2573 Fragments arranged by size: 2573 1156 1000 753 159 BmgBI CAC'GTC Cuts at: 1318 2044 1318 Size: 726 4915 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2712 3372 4330 Size: 416 53 83 399 24 1295 660 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1295 958 660 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2891 4301 1239 Size: 935 717 1410 2579 Fragments arranged by size: 2579 1410 935 717 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3766 4631 4929 2744 Size: 1022 865 298 3456 Fragments arranged by size: 3456 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2059 2242 2335 3432 493 Size: 711 855 183 93 1097 2702 Fragments arranged by size: 2702 1097 855 711 183 93 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2685 3448 3830 4465 Size: 53 83 186 936 1152 763 382 635 Cuts at: 4465 275 Size: 1451 Fragments arranged by size: 1451 1152 936 763 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2661 4074 4472 4746 4893 5304 2661 Size: 1413 398 274 147 411 2998 Fragments arranged by size: 2998 1413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2561 2591 1178 Size: 30 165 30 597 30 531 30 4228 Fragments arranged by size: 4228 597 531 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2027 2635 2638 1186 Size: 838 3 608 3 4189 Fragments arranged by size: 4189 838 608 3 3 BseYI C'CCAG_C Cuts at: 2608 2855 4844 5340 2608 Size: 247 1989 496 2909 Fragments arranged by size: 2909 1989 496 247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2513 2906 1163 Size: 573 582 195 393 3898 Fragments arranged by size: 3898 582 573 393 195 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2190 2247 3702 3787 4858 2028 Size: 162 57 1455 85 1071 2811 Fragments arranged by size: 2811 1455 1071 162 85 57 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2190 2247 2543 2702 2960 3702 Size: 399 238 162 57 296 159 258 742 Cuts at: 3702 3787 4858 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 296 258 238 162 159 85 57 BspEI T'CCGG_A Cuts at: 2661 5304 2661 Size: 2643 2998 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2080 2580 2996 844 Size: 1236 500 416 3489 Fragments arranged by size: 3489 1236 500 416 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2728 2869 2938 2287 Size: 441 141 69 4990 Fragments arranged by size: 4990 441 141 69 BstYI r'GATC_y Cuts at: 1972 2487 2971 3338 3723 3740 5192 5278 5290 Size: 515 484 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 515 484 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2210 2210 Size: 5641 BtgI C'CryG_G Cuts at: 513 1065 2004 2050 2280 2550 2643 2931 513 Size: 552 939 46 230 270 93 288 3223 Fragments arranged by size: 3223 939 552 288 270 230 93 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2552 4648 809 Size: 507 1236 2096 1802 Fragments arranged by size: 2096 1802 1236 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2277 2479 Size: 54 1425 50 275 18 243 204 202 Cuts at: 2479 3088 3977 5606 8 Size: 609 889 1629 43 Fragments arranged by size: 1629 1425 889 609 275 243 204 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3571 1353 Size: 2218 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2188 2188 Size: 5641 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2327 2891 3704 4301 5088 5356 Size: 393 542 153 564 813 597 787 268 Cuts at: 5356 1239 Size: 1524 Fragments arranged by size: 1524 813 787 597 564 542 393 268 153 EcoO109I rG'GnC_Cy Cuts at: 2395 2540 2707 2908 2395 Size: 145 167 201 5128 Fragments arranged by size: 5128 201 167 145 EcoRI G'AATT_C Cuts at: 2176 2950 3328 2176 Size: 774 378 4489 Fragments arranged by size: 4489 774 378 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 2614 2646 4282 Size: 32 135 32 989 32 21 32 1636 Cuts at: 4282 4314 4356 4388 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1636 989 135 42 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2592 4755 4939 1305 Size: 1287 2163 184 2007 Fragments arranged by size: 2163 2007 1287 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 10 Size: 54 1425 343 447 3372 Fragments arranged by size: 3372 1425 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2663 3599 3958 4117 518 Size: 648 1119 378 936 359 159 2042 Fragments arranged by size: 2042 1119 936 648 378 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2086 2636 2869 3057 3734 4882 5127 Size: 446 34 550 233 188 677 1148 245 Cuts at: 5127 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 550 446 245 233 188 34 NcoI C'CATG_G Cuts at: 513 1065 2004 2280 513 Size: 552 939 276 3874 Fragments arranged by size: 3874 939 552 276 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2527 2999 4544 1132 Size: 97 1298 472 1545 2229 Fragments arranged by size: 2229 1545 1298 472 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PfoI T'CCnGG_A Cuts at: 2190 2388 2190 Size: 198 5443 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 2707 2908 2395 Size: 312 201 5128 Fragments arranged by size: 5128 312 201 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2539 2539 Size: 5641 PstI C_TGCA'G Cuts at: 830 2091 2808 2993 830 Size: 1261 717 185 3478 Fragments arranged by size: 3478 1261 717 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2869 2869 Size: 5641 SacI G_AGCT'C Cuts at: 2190 2190 Size: 5641 SacII CC_GC'GG Cuts at: 2053 2053 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2087 2804 2989 4124 4805 4996 826 Size: 219 1042 717 185 1135 681 191 1471 Fragments arranged by size: 1471 1135 1042 717 681 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 3745 4646 4908 820 Size: 197 926 816 986 901 262 1553 Fragments arranged by size: 1553 986 926 901 816 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 2208 1517 Size: 691 4950 StyI C'CwwG_G Cuts at: 513 1065 2004 2280 3080 513 Size: 552 939 276 800 3074 Fragments arranged by size: 3074 939 800 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 3668 3685 3838 4023 4468 325 Size: 1255 762 1326 17 153 185 445 1498 Fragments arranged by size: 1498 1326 1255 762 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3887 5599 96 Size: 274 80 33 51 1443 1910 1712 138 Fragments arranged by size: 1910 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 5601 5633 2000 Size: 32 3569 32 2008 Fragments arranged by size: 3569 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2687 2782 1316 Size: 1371 95 4175 Fragments arranged by size: 4175 1371 95 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI