Restriction map for
pFN21AB4900
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI BsoBI BspDI BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI Hin1I KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 1433 TCCGGA T^CCGGA
AcsI 1325 GAATTC [GA]^AATT[CT]
AflIII 527 ACATGT A^C[GA][CT]GT
AocI 1330 CCTCAGG CC^T[ACGT]AGG
ApoI 1325 GAATTC [GA]^AATT[CT]
Asp700I 525 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 83 TGGCCA TGG^CCA
BanII 22 GAGCCC G[GA]GC[CT]^C
BclI 518 TGATCA T^GATCA
BcnI 225 CCGGG CC^[GC]GG
BpmI 303 CTCCAG CTGGAG(16/14)
BsaWI 1433 TCCGGA [AT]^CCGG[AT]
BseAI 1433 TCCGGA T^CCGGA
BsgI 97 GTGCAG GTGCAG(16/14)
Bsp1407I 947 TGTACA T^GTACA
BspEI 1433 TCCGGA T^CCGGA
BspHI 833 TCATGA T^CATGA
BspLU11I 527 ACATGT A^CATGT
BsrBI 1073 CCGCTC CCGCTC(-3/-3)
BsrGI 947 TGTACA T^GTACA
BstYI 706 GGATCT [GA]^GATC[CT]
Bsu36I 1330 CCTCAGG CC^T[ACGT]AGG
DrdI 1147 GACAAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 83 TGGCCA [CT]^GGCC[GA]
Eco81I 1330 CCTCAGG CC^T[ACGT]AGG
EcoNI 1209 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1325 GAATTC G^AATTC
FauI 1336 GCGGG CCCGC(4/6)
FbaI 518 TGATCA T^GATCA
HaeII 327 GGCGCT [GA]GCGC^[CT]
HgaI 1154 GCGTC GACGC(5/10)
HincII 1246 GTTAAC GT[CT]^[GA]AC
HindII 1246 GTTAAC GT[CT]^[GA]AC
HindIII 591 AAGCTT A^AGCTT
HpaI 1246 GTTAAC GTT^AAC
MaeII 1448 ACGT A^CGT
MflI 706 GGATCT [GA]^GATC[CT]
MluNI 83 TGGCCA TGG^CCA
MroI 1433 TCCGGA T^CCGGA
MscI 83 TGGCCA TGG^CCA
NciI 225 CCGGG CC^[GC]GG
NcoI 659 CCATGG C^CATGG
PpuMI 1081 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1365 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 497 CTGCAG CTGCA^G
RcaI 833 TCATGA T^CATGA
SapI 19 GAAGAGC GCTCTTC(1/4)
ScaI 236 AGTACT AGT^ACT
SmlI 1241 CTCAAG C^T[CT][GA]AG
SspBI 947 TGTACA T^GTACA
TaqI 1607 TCGA T^CGA
TthHB8I 1607 TCGA T^CGA
XhoII 706 GGATCT [GA]^GATC[CT]
XmnI 525 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1153 CGCG CG^CG
1407 CGCG
AlwI 706 GGATC GGATC(4/5)
1425 GGATC
AspHI 1168 GTGCTC G[AT]GC[AT]^C
1483 GAGCAC
BbsI 913 GAAGAC GAAGAC(2/6)
742 GTCTTC
BfaI 331 CTAG C^TAG
736 CTAG
BpuAI 913 GAAGAC GAAGAC(2/6)
742 GTCTTC
BseRI 1003 CTCCTC GAGGAG(10/8)
685 GAGGAG
BsiHKAI 1168 GTGCTC G[AT]GC[AT]^C
1483 GAGCAC
BsmI 141 GAATGC GAATGC(1/-1)
359 GAATGC
BstUI 1153 CGCG CG^CG
1407 CGCG
BstXI 1187 CCATTAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1445 CCAACGTACTGG
CfoI 328 GCGC GCG^C
1152 GCGC
DraII 794 AGGCCCT [GA]G^G[ACGT]CC[CT]
1081 GGGACCT
DsaI 659 CCATGG C^C[GA][CT]GG
869 CCGTGG
EcoO109I 794 AGGCCCT [GA]G^G[ACGT]CC[CT]
1081 GGGACCT
HapII 225 CCGG C^CGG
1434 CCGG
HhaI 328 GCGC GCG^C
1152 GCGC
HinP1I 328 GCGC G^CGC
1152 GCGC
HpaII 225 CCGG C^CGG
1434 CCGG
MaeI 331 CTAG C^TAG
736 CTAG
MspI 225 CCGG C^CGG
1434 CCGG
MvnI 1153 CGCG CG^CG
1407 CGCG
NspI 461 ACATGC [GA]CATG^[CT]
527 ACATGT
SfcI 368 CTGTAG C^T[GA][CT]AG
497 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI