(Circular) (Six-base) MAPSORT of: pFN21AB4900.seq Check: 8153 from: 1 to: 6331 pFN21AB4900 6331 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4141 278 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3674 1937 Size: 1737 4594 Acc65I G'GTAC_C Cuts at: 3652 3652 Size: 6331 AclI AA'CG_TT Cuts at: 1563 4458 4831 1563 Size: 2895 373 3063 Fragments arranged by size: 3063 2895 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3530 4394 5778 6046 1632 Size: 1898 864 1384 268 1917 Fragments arranged by size: 1917 1898 1384 864 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6134 AflIII A'CryG_T Cuts at: 1203 2532 5230 6207 1203 Size: 1329 2698 977 1327 Fragments arranged by size: 2698 1329 1327 977 AgeI A'CCGG_T Cuts at: 5162 5162 Size: 6331 AhdI GACnn_n'nnGTC Cuts at: 5060 5060 Size: 6331 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6331 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6331 AlwNI CAG_nnn'CTG Cuts at: 2287 2714 2803 4074 5646 2287 Size: 427 89 1271 1572 2972 Fragments arranged by size: 2972 1572 1271 427 89 ApaLI G'TGCA_C Cuts at: 4388 5544 4388 Size: 1156 5175 ApoI r'AATT_y Cuts at: 1493 3330 3640 3866 4018 6127 1493 Size: 1837 310 226 152 2109 1697 Fragments arranged by size: 2109 1837 1697 310 226 152 AseI AT'TA_AT Cuts at: 160 1032 4885 160 Size: 872 3853 1606 Fragments arranged by size: 3853 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 AvaI C'yCGr_G Cuts at: 1870 1943 3656 1870 Size: 73 1713 4545 Fragments arranged by size: 4545 1713 73 BamHI G'GATC_C Cuts at: 3661 4028 3661 Size: 367 5964 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3652 5107 618 Size: 325 217 627 1865 1455 1842 Fragments arranged by size: 1865 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2031 3650 1391 Size: 640 1619 4072 Fragments arranged by size: 4072 1619 640 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2740 2925 928 Size: 971 841 185 4334 Fragments arranged by size: 4334 971 841 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4522 4556 1903 Size: 34 2585 34 3678 Fragments arranged by size: 3678 2585 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4223 5433 4223 Size: 1210 5121 BclI T'GATC_A Cuts at: 1548 2523 1548 Size: 975 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4942 136 Size: 107 122 71 631 605 3270 1525 Fragments arranged by size: 3270 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6326 6326 Size: 6331 BlpI GC'TnA_GC Cuts at: 3748 3748 Size: 6331 Bme1580I G_kGCm'C Cuts at: 1790 2456 2610 4392 5548 1790 Size: 666 154 1782 1156 2573 Fragments arranged by size: 2573 1782 1156 666 154 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6331 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2696 3467 4062 Size: 416 53 83 399 24 1279 771 595 Cuts at: 4062 5020 442 Size: 958 1753 Fragments arranged by size: 1753 1279 958 771 595 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2293 4991 1239 Size: 1054 2698 2579 Fragments arranged by size: 2698 2579 1054 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3231 4456 5321 5619 3231 Size: 1225 865 298 3943 Fragments arranged by size: 3943 1225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4994 882 Size: 617 3495 2219 Fragments arranged by size: 3495 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4122 493 Size: 711 2918 2702 Fragments arranged by size: 2918 2702 711 BsaBI GATnn'nnATC Cuts at: 6325 6325 Size: 6331 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4138 4520 5155 275 Size: 53 83 186 936 2605 382 635 1451 Fragments arranged by size: 2605 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3438 4764 5162 5436 5583 5994 3438 Size: 1326 398 274 147 411 3775 Fragments arranged by size: 3775 1326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3479 3509 1178 Size: 30 165 30 2076 30 4000 Fragments arranged by size: 4000 2076 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2705 2999 1186 Size: 1519 294 4518 Fragments arranged by size: 4518 1519 294 BseYI C'CCAG_C Cuts at: 2897 3476 5534 6030 2897 Size: 579 2058 496 3198 Fragments arranged by size: 3198 2058 579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 5371 Fragments arranged by size: 5371 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3781 4542 5570 1540 Size: 275 186 1780 761 1028 2301 Fragments arranged by size: 2301 1780 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3177 3492 4392 4477 5548 3177 Size: 315 900 85 1071 3960 Fragments arranged by size: 3960 1071 900 315 85 BsmI GAATG_Cn' Cuts at: 2152 2370 3842 3935 2152 Size: 218 1472 93 4548 Fragments arranged by size: 4548 1472 218 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2456 2610 3177 3492 3650 4392 Size: 399 241 425 154 567 315 158 742 Cuts at: 4392 4477 5548 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 425 399 315 241 158 154 85 BspEI T'CCGG_A Cuts at: 3438 5994 3438 Size: 2556 3775 BspHI T'CATG_A Cuts at: 2838 4220 5950 2838 Size: 1382 1730 3219 Fragments arranged by size: 3219 1730 1382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3686 844 Size: 2842 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3080 4218 1394 Size: 429 1257 1138 3507 Fragments arranged by size: 3507 1257 1138 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2023 2400 3240 4826 5000 65 Size: 1183 775 377 840 1586 174 1396 Fragments arranged by size: 1586 1396 1183 840 775 377 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4975 5162 1700 Size: 110 3165 187 2869 Fragments arranged by size: 3165 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2952 96 Size: 2856 3475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6331 BssSI C'ACGA_G Cuts at: 4391 5403 4391 Size: 1012 5319 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6331 BstBI TT'CG_AA Cuts at: 5135 5135 Size: 6331 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6331 BstXI CCAn_nnnn'nTGG Cuts at: 3199 3457 3199 Size: 258 6073 BstYI r'GATC_y Cuts at: 1972 2711 3661 4028 4413 4430 5882 5968 5980 Size: 739 950 367 385 17 1452 86 12 Cuts at: 5980 6326 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 950 739 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3336 3336 Size: 6331 BtgI C'CryG_G Cuts at: 513 1065 2664 2874 513 Size: 552 1599 210 3970 Fragments arranged by size: 3970 1599 552 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3394 487 Size: 683 56 2168 3424 Fragments arranged by size: 3424 2168 683 56 BtsI GCAGTG_nn' Cuts at: 3847 4641 4661 3847 Size: 794 20 5517 Fragments arranged by size: 5517 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2383 2418 529 Size: 35 1819 35 4442 Fragments arranged by size: 4442 1819 35 35 DraI TTT'AAA Cuts at: 2566 2575 2602 3635 2566 Size: 9 27 1033 5262 Fragments arranged by size: 5262 1033 27 9 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3158 5338 809 Size: 507 1842 2180 1802 Fragments arranged by size: 2180 1842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 3778 4667 Size: 54 1425 50 275 18 258 1690 889 Cuts at: 4667 6296 8 Size: 1629 43 Fragments arranged by size: 1690 1629 1425 889 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3778 1537 Size: 275 1966 4090 Fragments arranged by size: 4090 1966 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2585 2891 3097 3129 3543 4261 1353 Size: 666 566 306 206 32 414 718 3423 Fragments arranged by size: 3423 718 666 566 414 306 206 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4918 5292 5438 387 Size: 1064 138 3329 374 146 1280 Fragments arranged by size: 3329 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2293 3530 4394 4991 5778 6046 1239 Size: 393 661 1237 864 597 787 268 1524 Fragments arranged by size: 1524 1237 864 787 661 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3218 3218 Size: 6331 EcoO109I rG'GnC_Cy Cuts at: 2800 3087 2800 Size: 287 6044 EcoRI G'AATT_C Cuts at: 3330 3640 4018 3330 Size: 310 378 5643 Fragments arranged by size: 5643 378 310 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 6331 FspI TGC'GCA Cuts at: 4837 4837 Size: 6331 HaeII r_GCGC'y Cuts at: 1678 2336 5478 1678 Size: 658 3142 2531 Fragments arranged by size: 3142 2531 658 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 2361 2393 3159 Size: 32 135 32 618 32 139 32 766 Cuts at: 3159 3191 3479 3511 4972 5004 5046 5078 1373 Size: 32 288 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 766 618 288 139 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3253 3675 3921 669 Size: 988 281 1315 422 246 3079 Fragments arranged by size: 3079 1315 988 422 281 246 HindIII A'AGCT_T Cuts at: 748 2596 748 Size: 1848 4483 HpaI GTT'AAC Cuts at: 3253 3921 3253 Size: 668 5663 KpnI G_GTAC'C Cuts at: 3656 3656 Size: 6331 MfeI C'AATT_G Cuts at: 3930 3930 Size: 6331 MluI A'CGCG_T Cuts at: 6207 6207 Size: 6331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2233 5445 5629 1305 Size: 928 3212 184 2007 Fragments arranged by size: 3212 2007 928 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 10 Size: 54 1425 343 258 4251 Fragments arranged by size: 4251 1425 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2324 2405 2927 3197 3413 4289 4648 Size: 648 1158 81 522 270 216 876 359 Cuts at: 4648 4807 518 Size: 159 2042 Fragments arranged by size: 2042 1158 876 648 522 359 270 216 159 81 MspA1I CmG'CkG Cuts at: 1606 2174 2501 3308 3326 3747 4424 5572 5817 Size: 568 327 807 18 421 677 1148 245 Cuts at: 5817 1606 Size: 2120 Fragments arranged by size: 2120 1148 807 677 568 421 327 245 18 NcoI C'CATG_G Cuts at: 513 1065 2664 513 Size: 552 1599 4180 Fragments arranged by size: 4180 1599 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6331 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6331 NotI GC'GGCC_GC Cuts at: 3778 3778 Size: 6331 NspI r_CATG'y Cuts at: 1132 1229 2470 2536 3689 5234 1132 Size: 97 1241 66 1153 1545 2229 Fragments arranged by size: 2229 1545 1241 1153 97 66 PasI CC'CwG_GG Cuts at: 1348 2734 3469 1348 Size: 1386 735 4210 Fragments arranged by size: 4210 1386 735 PciI A'CATG_T Cuts at: 2532 5230 2532 Size: 2698 3633 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6331 PfoI T'CCnGG_A Cuts at: 2514 2707 3426 2514 Size: 193 719 5419 Fragments arranged by size: 5419 719 193 PmeI GTTT'AAAC Cuts at: 3635 3635 Size: 6331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 5913 5945 4400 Size: 32 1481 32 4786 Fragments arranged by size: 4786 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3087 3087 Size: 6331 PshAI GACnn'nnGTC Cuts at: 1613 3374 1613 Size: 1761 4570 PsiI TTA'TAA Cuts at: 3901 3901 Size: 6331 PstI C_TGCA'G Cuts at: 830 2506 3683 830 Size: 1676 1177 3478 Fragments arranged by size: 3478 1676 1177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6331 PvuII CAG'CTG Cuts at: 2501 3308 3326 2501 Size: 807 18 5506 Fragments arranged by size: 5506 807 18 SalI G'TCGA_C Cuts at: 1936 3673 1936 Size: 1737 4594 SapI GCTCTTCn'nnn_ Cuts at: 2019 2019 Size: 6331 SbfI CC_TGCA'GG Cuts at: 3683 3683 Size: 6331 ScaI AGT'ACT Cuts at: 2243 4579 6291 2243 Size: 2336 1712 2283 Fragments arranged by size: 2336 2283 1712 SfcI C'TryA_G Cuts at: 826 1045 2373 2502 3679 4814 5495 5686 826 Size: 219 1328 129 1177 1135 681 191 1471 Fragments arranged by size: 1471 1328 1177 1135 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6331 SmaI CCC'GGG Cuts at: 1872 3658 1872 Size: 1786 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3246 4435 5336 5598 820 Size: 197 926 1303 1189 901 262 1553 Fragments arranged by size: 1553 1303 1189 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4122 493 Size: 3629 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6331 SphI G_CATG'C Cuts at: 1132 3689 1132 Size: 2557 3774 SspI AAT'ATT Cuts at: 5 52 4255 5 Size: 47 4203 2081 Fragments arranged by size: 4203 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6331 StyI C'CwwG_G Cuts at: 513 1065 2194 2664 3038 3770 513 Size: 552 1129 470 374 732 3074 Fragments arranged by size: 3074 1129 732 552 470 374 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2624 2915 4358 4375 4528 4713 5158 Size: 1255 1044 291 1443 17 153 185 445 Cuts at: 5158 325 Size: 1498 Fragments arranged by size: 1498 1443 1255 1044 445 291 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2952 4577 Size: 274 80 33 51 1443 264 711 1625 Cuts at: 4577 6289 96 Size: 1712 138 Fragments arranged by size: 1712 1625 1443 711 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6291 6323 6291 Size: 32 6299 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6331 XbaI T'CTAG_A Cuts at: 3667 3667 Size: 6331 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2404 2842 3196 3454 2404 Size: 438 354 258 5281 Fragments arranged by size: 5281 438 354 258 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6331 XmaI C'CCGG_G Cuts at: 1870 3656 1870 Size: 1786 4545 XmnI GAAnn'nnTTC Cuts at: 2534 4460 2534 Size: 1926 4405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4139 276 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI