(Circular) (Six-base) MAPSORT of: pFN21AB4835.seq Check: 8137 from: 1 to: 6139 pFN21AB4835 6139 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2688 2749 3144 2688 Size: 61 395 5683 Fragments arranged by size: 5683 395 61 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 2531 3460 2531 Size: 929 5210 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2531 2856 3344 4202 5586 5854 1632 Size: 899 325 488 858 1384 268 1917 Fragments arranged by size: 1917 1384 899 858 488 325 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2730 5038 6015 1203 Size: 1527 2308 977 1327 Fragments arranged by size: 2308 1527 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 2051 1166 Size: 885 5254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3221 3253 3221 Size: 32 6107 AlwNI CAG_nnn'CTG Cuts at: 3232 3882 5454 3232 Size: 650 1572 3917 Fragments arranged by size: 3917 1572 650 ApaI G_GGCC'C Cuts at: 2169 2169 Size: 6139 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 2408 3448 3674 3826 5935 1493 Size: 915 1040 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 915 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2358 2662 3201 3464 1870 Size: 73 415 304 539 263 4545 Fragments arranged by size: 4545 539 415 304 263 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2531 3132 3460 4915 Size: 325 217 627 255 489 601 328 1455 Cuts at: 4915 618 Size: 1842 Fragments arranged by size: 1842 1455 627 601 489 328 325 255 217 BanII G_rGCy'C Cuts at: 1391 2169 2358 2663 3208 3458 1391 Size: 778 189 305 545 250 4072 Fragments arranged by size: 4072 778 545 305 250 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5168 BbvCI CC'TCA_GC Cuts at: 2069 2069 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2204 2238 2579 2613 4330 4364 1903 Size: 34 267 34 341 34 1717 34 3678 Fragments arranged by size: 3678 1717 341 267 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BfrBI ATG'CAT Cuts at: 2284 2284 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2173 4750 136 Size: 107 122 71 631 605 501 2577 1525 Fragments arranged by size: 2577 1525 631 605 501 122 107 71 BglII A'GATC_T Cuts at: 3022 6134 3022 Size: 3112 3027 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2045 2122 2169 3195 4200 5356 1790 Size: 255 77 47 1026 1005 1156 2573 Fragments arranged by size: 2573 1156 1026 1005 255 77 47 BmgBI CAC'GTC Cuts at: 1318 3319 1318 Size: 2001 4138 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2906 3870 4828 Size: 416 53 83 399 24 1489 964 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1753 1489 964 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2880 3053 3245 4799 1239 Size: 1641 173 192 1554 2579 Fragments arranged by size: 2579 1641 1554 192 173 Bpu10I CC'TnA_GC Cuts at: 2069 2069 Size: 6139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2382 3196 3930 493 Size: 711 1178 814 734 2702 Fragments arranged by size: 2702 1178 814 734 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 4328 4963 275 Size: 53 83 186 936 2413 382 635 1451 Fragments arranged by size: 2413 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 3411 4572 4970 5244 5391 5802 2247 Size: 1164 1161 398 274 147 411 2584 Fragments arranged by size: 2584 1164 1161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2229 2259 2650 2680 3221 Size: 30 165 30 826 30 391 30 541 Cuts at: 3221 3251 1178 Size: 30 4066 Fragments arranged by size: 4066 826 541 391 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2671 3160 3163 1186 Size: 1485 489 3 4162 Fragments arranged by size: 4162 1485 489 3 BseYI C'CCAG_C Cuts at: 2241 2427 2607 3121 5342 5838 2241 Size: 186 180 514 2221 496 2542 Fragments arranged by size: 2542 2221 514 496 186 180 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2871 3101 3218 3356 1163 Size: 573 1135 230 117 138 3946 Fragments arranged by size: 3946 1135 573 230 138 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2148 3589 4350 5378 1540 Size: 275 186 17 130 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 130 17 BsiHKAI G_wGCw'C Cuts at: 2663 4200 4285 5356 2663 Size: 1537 85 1071 3446 Fragments arranged by size: 3446 1537 1071 85 BsmI GAATG_Cn' Cuts at: 2602 3650 3743 2602 Size: 1048 93 4998 Fragments arranged by size: 4998 1048 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2122 2169 2358 2663 3195 3208 Size: 399 255 77 47 189 305 532 13 Cuts at: 3208 3458 4200 4285 5356 1391 Size: 250 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 399 305 255 250 189 85 77 47 13 BspEI T'CCGG_A Cuts at: 2247 3411 5802 2247 Size: 1164 2391 2584 Fragments arranged by size: 2584 2391 1164 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2688 2749 3144 3240 3494 844 Size: 1844 61 395 96 254 3489 Fragments arranged by size: 3489 1844 395 254 96 61 BsrBI CCG'CTC Cuts at: 1394 1823 4026 1394 Size: 429 2203 3507 Fragments arranged by size: 3507 2203 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2983 4634 4808 65 Size: 1183 1735 1651 174 1396 Fragments arranged by size: 1735 1651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2528 4783 4970 1700 Size: 110 718 2255 187 2869 Fragments arranged by size: 2869 2255 718 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 2213 4199 5211 2213 Size: 1986 1012 3141 Fragments arranged by size: 3141 1986 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3241 3334 794 Size: 2447 93 3599 Fragments arranged by size: 3599 2447 93 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2285 2285 Size: 6139 BstYI r'GATC_y Cuts at: 1972 3022 3469 3836 4221 4238 5690 5776 5788 Size: 1050 447 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1050 447 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2454 513 Size: 552 939 450 4198 Fragments arranged by size: 4198 939 552 450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2677 487 Size: 683 56 1451 3949 Fragments arranged by size: 3949 1451 683 56 BtsI GCAGTG_nn' Cuts at: 2109 2713 3298 3655 4449 4469 2109 Size: 604 585 357 794 20 3779 Fragments arranged by size: 3779 794 604 585 357 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2160 2392 Size: 54 1425 50 275 18 185 145 232 Cuts at: 2392 2451 2955 2985 3586 4475 6104 8 Size: 59 504 30 601 889 1629 43 Fragments arranged by size: 1629 1425 889 601 504 275 232 185 145 59 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2015 3586 1537 Size: 275 203 1571 4090 Fragments arranged by size: 4090 1571 275 203 EarI CTCTTCn'nnn_ Cuts at: 1353 4069 1353 Size: 2716 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2661 2661 Size: 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 2856 2880 3053 3245 3344 4202 Size: 393 899 325 24 173 192 99 858 Cuts at: 4202 4799 5586 5854 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 899 858 787 597 393 325 268 192 173 99 24 EcoO109I rG'GnC_Cy Cuts at: 2166 2940 2166 Size: 774 5365 EcoRI G'AATT_C Cuts at: 2408 3448 3826 2408 Size: 1040 378 4721 Fragments arranged by size: 4721 1040 378 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 5286 1678 Size: 3608 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2229 2261 3007 Size: 32 135 32 503 32 122 32 746 Cuts at: 3007 3039 3190 3222 4780 4812 4854 4886 1373 Size: 32 151 32 1558 32 42 32 2626 Fragments arranged by size: 2626 1558 746 503 151 135 122 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 2535 3464 2535 Size: 929 5210 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 3201 5253 5437 1305 Size: 1308 588 2052 184 2007 Fragments arranged by size: 2052 2007 1308 588 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 2987 10 Size: 54 1425 343 621 534 3162 Fragments arranged by size: 3162 1425 621 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2267 2445 2544 4097 4456 4615 Size: 648 885 216 178 99 1553 359 159 Cuts at: 4615 518 Size: 2042 Fragments arranged by size: 2042 1553 885 648 359 216 178 159 99 MspA1I CmG'CkG Cuts at: 1606 2607 2854 3077 3148 3386 3555 4232 5380 Size: 1001 247 223 71 238 169 677 1148 Cuts at: 5380 5625 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1001 677 247 245 238 223 169 71 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4648 Fragments arranged by size: 4648 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NsiI A_TGCA'T Cuts at: 2286 2286 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 2734 3004 3497 5042 1132 Size: 97 1505 270 493 1545 2229 Fragments arranged by size: 2229 1545 1505 493 270 97 PasI CC'CwG_GG Cuts at: 1348 2170 3187 1348 Size: 822 1017 4300 Fragments arranged by size: 4300 1017 822 PciI A'CATG_T Cuts at: 2730 5038 2730 Size: 2308 3831 PflMI CCAn_nnn'nTGG Cuts at: 1108 2481 3004 1108 Size: 1373 523 4243 Fragments arranged by size: 4243 1373 523 PfoI T'CCnGG_A Cuts at: 3261 3308 3261 Size: 47 6092 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PmlI CAC'GTG Cuts at: 2382 3196 2382 Size: 814 5325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3207 3221 3239 3253 4208 4240 5721 5753 3207 Size: 14 18 14 955 32 1481 32 3593 Fragments arranged by size: 3593 1481 955 32 32 18 14 14 PpuMI rG'GwC_Cy Cuts at: 2940 2940 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 2165 2165 Size: 6139 PstI C_TGCA'G Cuts at: 830 3491 830 Size: 2661 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2607 2854 3077 3148 3386 2607 Size: 247 223 71 238 5360 Fragments arranged by size: 5360 247 238 223 71 SacI G_AGCT'C Cuts at: 2663 2663 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SexAI A'CCwGG_T Cuts at: 2373 2373 Size: 6139 SfcI C'TryA_G Cuts at: 826 1045 3487 4622 5303 5494 826 Size: 219 2442 1135 681 191 1471 Fragments arranged by size: 2442 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3203 3466 1872 Size: 1331 263 4545 Fragments arranged by size: 4545 1331 263 SmlI C'TyrA_G Cuts at: 820 1017 1943 4243 5144 5406 820 Size: 197 926 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2789 3030 1517 Size: 1272 241 4626 Fragments arranged by size: 4626 1272 241 StyI C'CwwG_G Cuts at: 513 1065 2004 2311 2772 3578 513 Size: 552 939 307 461 806 3074 Fragments arranged by size: 3074 939 806 552 461 307 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2765 4166 4183 4336 4521 4966 325 Size: 1255 1185 1401 17 153 185 445 1498 Fragments arranged by size: 1498 1401 1255 1185 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4385 6097 96 Size: 274 80 33 51 1443 2408 1712 138 Fragments arranged by size: 2408 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3201 3464 1870 Size: 1331 263 4545 Fragments arranged by size: 4545 1331 263 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI