(Circular) (Six-base) MAPSORT of: pFN21AB4828.seq Check: 2021 from: 1 to: 5578 pFN21AB4828 5578 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2450 3388 278 Size: 53 83 186 936 914 938 2468 Fragments arranged by size: 2468 938 936 914 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 2921 1937 Size: 130 854 4594 Fragments arranged by size: 4594 854 130 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 2868 3641 5025 5293 1632 Size: 800 436 773 1384 268 1917 Fragments arranged by size: 1917 1384 800 773 436 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 2504 4477 5454 1203 Size: 1301 1973 977 1327 Fragments arranged by size: 1973 1327 1301 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 2507 1166 Size: 1341 4237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2033 1184 Size: 849 4729 AlwNI CAG_nnn'CTG Cuts at: 2250 2846 3321 4893 2250 Size: 596 475 1572 2935 Fragments arranged by size: 2935 1572 596 475 ApaLI G'TGCA_C Cuts at: 2321 3635 4791 2321 Size: 1314 1156 3108 Fragments arranged by size: 3108 1314 1156 ApoI r'AATT_y Cuts at: 1493 2887 3113 3265 5374 1493 Size: 1394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2903 1870 Size: 73 960 4545 Fragments arranged by size: 4545 960 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2899 4354 618 Size: 325 217 627 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 217 BanII G_rGCy'C Cuts at: 1391 2279 2357 2379 2778 2897 1391 Size: 888 78 22 399 119 4072 Fragments arranged by size: 4072 888 399 119 78 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 2856 928 Size: 971 221 736 3650 Fragments arranged by size: 3650 971 736 221 BbvCI CC'TCA_GC Cuts at: 2547 2547 Size: 5578 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 2283 1548 Size: 735 4843 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2025 5573 2025 Size: 3548 2030 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 2325 3639 4795 1790 Size: 535 1314 1156 2573 Fragments arranged by size: 2573 1314 1156 535 BmgBI CAC'GTC Cuts at: 1318 2491 2875 1318 Size: 1173 384 4021 Fragments arranged by size: 4021 1173 384 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4267 442 Size: 416 53 83 399 24 1892 958 1753 Fragments arranged by size: 1892 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2877 4238 1239 Size: 1638 1361 2579 Fragments arranged by size: 2579 1638 1361 Bpu10I CC'TnA_GC Cuts at: 2547 2547 Size: 5578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 3703 4568 4866 2675 Size: 1028 865 298 3387 Fragments arranged by size: 3387 1028 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 4241 882 Size: 617 897 1845 2219 Fragments arranged by size: 2219 1845 897 617 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2447 3385 3767 4402 Size: 53 83 186 936 914 938 382 635 Cuts at: 4402 275 Size: 1451 Fragments arranged by size: 1451 938 936 914 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2191 2630 4011 4409 4683 4830 5241 2191 Size: 439 1381 398 274 147 411 2528 Fragments arranged by size: 2528 1381 439 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5353 Fragments arranged by size: 5353 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 1186 Size: 1378 4200 BseYI C'CCAG_C Cuts at: 2159 4781 5277 2159 Size: 2622 496 2460 Fragments arranged by size: 2622 2460 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2284 1163 Size: 573 548 4457 Fragments arranged by size: 4457 573 548 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3028 3789 4817 1540 Size: 275 186 1027 761 1028 2301 Fragments arranged by size: 2301 1028 1027 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2357 2379 2477 2532 3639 3724 4795 2325 Size: 32 22 98 55 1107 85 1071 3108 Fragments arranged by size: 3108 1107 1071 98 85 55 32 22 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2279 2325 2357 2379 2477 2532 2778 Size: 399 489 46 32 22 98 55 246 Cuts at: 2778 2897 3639 3724 4795 1391 Size: 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 399 246 119 98 85 55 46 32 22 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 2130 3467 5197 2130 Size: 1337 1730 2511 Fragments arranged by size: 2511 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 844 Size: 2089 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3465 1394 Size: 429 1642 3507 Fragments arranged by size: 3507 1642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 4073 4247 65 Size: 1183 1245 1580 174 1396 Fragments arranged by size: 1580 1396 1245 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4222 4409 1700 Size: 110 2412 187 2869 Fragments arranged by size: 2869 2412 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 2250 794 Size: 1456 4122 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2025 2247 2908 3275 3660 3677 5129 5215 Size: 53 222 661 367 385 17 1452 86 Cuts at: 5215 5227 5573 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 661 385 367 346 222 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5026 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2296 2348 3094 3888 3908 2015 Size: 281 52 746 794 20 3685 Fragments arranged by size: 3685 794 746 281 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2320 2320 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 3025 3914 Size: 54 1425 50 275 18 786 409 889 Cuts at: 3914 5543 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 786 409 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 1537 Size: 275 1213 4090 Fragments arranged by size: 4090 1213 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 2833 3508 1353 Size: 1325 155 675 3423 Fragments arranged by size: 3423 1325 675 155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4165 4539 4685 387 Size: 1064 138 2576 374 146 1280 Fragments arranged by size: 2576 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2355 2377 2355 Size: 22 5556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2868 2877 3641 4238 5025 5293 Size: 393 800 436 9 764 597 787 268 Cuts at: 5293 1239 Size: 1524 Fragments arranged by size: 1524 800 787 764 597 436 393 268 9 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 4725 1678 Size: 3047 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2790 2810 2822 Size: 32 135 32 467 32 719 20 12 Cuts at: 2822 2842 2850 2882 4219 4251 4293 4325 1373 Size: 20 8 32 1337 32 42 32 2626 Fragments arranged by size: 2626 1337 719 467 135 42 32 32 32 32 32 32 20 20 12 8 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 2675 3177 2675 Size: 502 5076 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2382 2841 4692 4876 1305 Size: 1077 459 1851 184 2007 Fragments arranged by size: 2007 1851 1077 459 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 10 Size: 54 1425 343 786 2970 Fragments arranged by size: 2970 1425 786 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2507 2799 3536 3895 4054 518 Size: 648 843 498 292 737 359 159 2042 Fragments arranged by size: 2042 843 737 648 498 359 292 159 MspA1I CmG'CkG Cuts at: 1606 2994 3671 4819 5064 1606 Size: 1388 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5026 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2014 2508 2936 4481 1132 Size: 97 785 494 428 1545 2229 Fragments arranged by size: 2229 1545 785 494 428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5578 PciI A'CATG_T Cuts at: 2504 4477 2504 Size: 1973 3605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PstI C_TGCA'G Cuts at: 830 2246 2340 2930 830 Size: 1416 94 590 3478 Fragments arranged by size: 3478 1416 590 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 SacI G_AGCT'C Cuts at: 2357 2379 2357 Size: 22 5556 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SfcI C'TryA_G Cuts at: 826 1045 2068 2242 2336 2926 4061 4742 4933 Size: 219 1023 174 94 590 1135 681 191 Cuts at: 4933 826 Size: 1471 Fragments arranged by size: 1471 1135 1023 681 590 219 191 174 94 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2690 3682 4583 4845 820 Size: 197 926 747 992 901 262 1553 Fragments arranged by size: 1553 992 926 901 747 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2014 2936 1132 Size: 882 922 3774 Fragments arranged by size: 3774 922 882 SspI AAT'ATT Cuts at: 5 52 2749 3502 5 Size: 47 2697 753 2081 Fragments arranged by size: 2697 2081 753 47 StuI AGG'CCT Cuts at: 1517 2330 2696 1517 Size: 813 366 4399 Fragments arranged by size: 4399 813 366 StyI C'CwwG_G Cuts at: 513 1065 3017 513 Size: 552 1952 3074 Fragments arranged by size: 3074 1952 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3824 5536 96 Size: 274 80 33 51 1443 1847 1712 138 Fragments arranged by size: 1847 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5538 5570 5538 Size: 32 5546 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2424 2506 2424 Size: 82 5496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 2868 3707 2868 Size: 839 4739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2448 3386 276 Size: 53 83 186 936 914 938 2468 Fragments arranged by size: 2468 938 936 914 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI