(Circular) (Six-base) MAPSORT of: pFN21AB4722.seq Check: 5155 from: 1 to: 5695 pFN21AB4722 5695 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2721 2721 Size: 5695 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 2840 3758 5142 5410 1632 Size: 755 453 918 1384 268 1917 Fragments arranged by size: 1917 1384 918 755 453 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2093 1184 Size: 909 4786 AlwNI CAG_nnn'CTG Cuts at: 2516 2798 3438 5010 2516 Size: 282 640 1572 3201 Fragments arranged by size: 3201 1572 640 282 ApaLI G'TGCA_C Cuts at: 3752 4908 3752 Size: 1156 4539 ApoI r'AATT_y Cuts at: 1493 2654 3004 3230 3382 5491 1493 Size: 1161 350 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 350 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2428 3020 1870 Size: 73 485 592 4545 Fragments arranged by size: 4545 592 485 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2733 3016 4471 618 Size: 325 217 627 946 283 1455 1842 Fragments arranged by size: 1842 1455 946 627 325 283 217 BanII G_rGCy'C Cuts at: 1391 3014 1391 Size: 1623 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4306 136 Size: 107 122 71 631 605 2634 1525 Fragments arranged by size: 2634 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2342 5690 2342 Size: 3348 2347 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2376 3756 4912 1790 Size: 586 1380 1156 2573 Fragments arranged by size: 2573 1380 1156 586 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2504 3426 4384 Size: 416 53 83 399 24 1087 922 958 Cuts at: 4384 442 Size: 1753 Fragments arranged by size: 1753 1087 958 922 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2174 1056 Size: 1118 4577 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4355 1239 Size: 3116 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 3820 4685 4983 2468 Size: 1352 865 298 3180 Fragments arranged by size: 3180 1352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 3502 3884 4519 Size: 53 83 186 936 495 1474 382 635 Cuts at: 4519 275 Size: 1451 Fragments arranged by size: 1474 1451 936 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 1186 Size: 855 4840 BseYI C'CCAG_C Cuts at: 4898 5394 4898 Size: 496 5199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2894 1163 Size: 573 1158 3964 Fragments arranged by size: 3964 1158 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3756 3841 4912 3756 Size: 85 1071 4539 Fragments arranged by size: 4539 1071 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 BsmBI CGTCTCn'nnnn_ Cuts at: 2019 2019 Size: 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2376 3014 3756 3841 4912 1391 Size: 399 586 638 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 638 586 399 85 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2721 3050 844 Size: 1877 329 3489 Fragments arranged by size: 3489 1877 329 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4339 4526 1700 Size: 110 2529 187 2869 Fragments arranged by size: 2869 2529 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2342 2432 3025 3392 3777 3794 5246 5332 Size: 370 90 593 367 385 17 1452 86 Cuts at: 5332 5344 5690 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 593 385 370 367 346 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2923 487 Size: 683 56 1697 3259 Fragments arranged by size: 3259 1697 683 56 BtsI GCAGTG_nn' Cuts at: 2139 2682 3211 4005 4025 2139 Size: 543 529 794 20 3809 Fragments arranged by size: 3809 794 543 529 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2610 2999 2610 Size: 389 5306 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 3142 4031 Size: 54 1425 50 275 18 228 1084 889 Cuts at: 4031 5660 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1084 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3625 1353 Size: 2272 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2397 4282 4656 4802 387 Size: 1064 138 808 1885 374 146 1280 Fragments arranged by size: 1885 1280 1064 808 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2840 3758 4355 5142 5410 1239 Size: 393 755 453 918 597 787 268 1524 Fragments arranged by size: 1524 918 787 755 597 453 393 268 EcoO109I rG'GnC_Cy Cuts at: 2541 2541 Size: 5695 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 4842 1678 Size: 3164 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 2579 2611 4336 Size: 32 135 32 755 32 220 32 1725 Cuts at: 4336 4368 4410 4442 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1725 755 220 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 4809 4993 1305 Size: 1134 2370 184 2007 Fragments arranged by size: 2370 2007 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2760 2895 3653 4012 4171 518 Size: 648 1594 135 758 359 159 2042 Fragments arranged by size: 2042 1594 758 648 359 159 135 MspA1I CmG'CkG Cuts at: 1606 3111 3788 4936 5181 1606 Size: 1505 677 1148 245 2120 Fragments arranged by size: 2120 1505 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 NdeI CA'TA_TG Cuts at: 387 2215 387 Size: 1828 3867 NheI G'CTAG_C Cuts at: 1052 2170 1052 Size: 1118 4577 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 3053 4598 1132 Size: 97 1824 1545 2229 Fragments arranged by size: 2229 1824 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2541 2541 Size: 5695 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 2423 3265 2423 Size: 842 4853 PstI C_TGCA'G Cuts at: 830 3047 830 Size: 2217 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SexAI A'CCwGG_T Cuts at: 2835 2835 Size: 5695 SfcI C'TryA_G Cuts at: 826 1045 2575 2925 3043 4178 4859 5050 826 Size: 219 1530 350 118 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 681 350 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 3799 4700 4962 820 Size: 197 926 540 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 540 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 2771 3619 5 Size: 47 2719 848 2081 Fragments arranged by size: 2719 2081 848 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5695 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 3134 513 Size: 552 939 9 1121 3074 Fragments arranged by size: 3074 1121 939 552 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2108 3941 5653 Size: 274 80 33 51 1443 131 1833 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1833 1712 1443 274 138 131 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 2491 1316 Size: 1175 4520 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI