(Circular) (Six-base) MAPSORT of: pFN21AB4697.seq Check: 1042 from: 1 to: 5686 pFN21AB4697 5686 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2899 2899 Size: 5686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2478 3029 1937 Size: 541 551 4594 Fragments arranged by size: 4594 551 541 Acc65I G'GTAC_C Cuts at: 2715 3007 2715 Size: 292 5394 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2813 2957 3749 5133 5401 1632 Size: 1181 144 792 1384 268 1917 Fragments arranged by size: 1917 1384 1181 792 268 144 AflII C'TTAA_G Cuts at: 820 1017 2669 820 Size: 197 1652 3837 Fragments arranged by size: 3837 1652 197 AflIII A'CryG_T Cuts at: 1203 2775 4585 5562 1203 Size: 1572 1810 977 1327 Fragments arranged by size: 1810 1572 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2369 1184 Size: 1185 4501 AlwNI CAG_nnn'CTG Cuts at: 2035 2935 3429 5001 2035 Size: 900 494 1572 2720 Fragments arranged by size: 2720 1572 900 494 ApaI G_GGCC'C Cuts at: 2160 2160 Size: 5686 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2663 2995 3221 3373 5482 1493 Size: 1170 332 226 152 2109 1697 Fragments arranged by size: 2109 1697 1170 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 2503 2731 3011 1870 Size: 73 560 228 280 4545 Fragments arranged by size: 4545 560 280 228 73 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2132 2715 3007 4462 Size: 325 217 627 303 42 583 292 1455 Cuts at: 4462 618 Size: 1842 Fragments arranged by size: 1842 1455 627 583 325 303 292 217 42 BanII G_rGCy'C Cuts at: 1391 2160 2572 2853 3005 1391 Size: 769 412 281 152 4072 Fragments arranged by size: 4072 769 412 281 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2529 2670 928 Size: 971 424 206 141 3944 Fragments arranged by size: 3944 971 424 206 141 BbvCI CC'TCA_GC Cuts at: 2342 2342 Size: 5686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2419 2453 3877 3911 1903 Size: 34 482 34 1424 34 3678 Fragments arranged by size: 3678 1424 482 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 2160 2241 3747 4903 1790 Size: 370 81 1506 1156 2573 Fragments arranged by size: 2573 1506 1156 370 81 BmgBI CAC'GTC Cuts at: 1318 2776 2953 1318 Size: 1458 177 4051 Fragments arranged by size: 4051 1458 177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3417 4375 442 Size: 416 53 83 399 24 2000 958 1753 Fragments arranged by size: 2000 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2308 2752 4346 1239 Size: 856 213 444 1594 2579 Fragments arranged by size: 2579 1594 856 444 213 Bpu10I CC'TnA_GC Cuts at: 2342 2342 Size: 5686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2728 2840 3811 4676 4974 2728 Size: 112 971 865 298 3440 Fragments arranged by size: 3440 971 865 298 112 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2585 4349 882 Size: 617 1086 1764 2219 Fragments arranged by size: 2219 1764 1086 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2601 2675 3493 3875 Size: 53 83 186 936 1068 74 818 382 Cuts at: 3875 4510 275 Size: 635 1451 Fragments arranged by size: 1451 1068 936 818 635 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 2247 4119 4517 4791 4938 5349 2247 Size: 1872 398 274 147 411 2584 Fragments arranged by size: 2584 1872 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 1186 Size: 1108 4578 BseYI C'CCAG_C Cuts at: 2362 4889 5385 2362 Size: 2527 496 2663 Fragments arranged by size: 2663 2527 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 2049 3136 3897 4925 1540 Size: 275 186 19 29 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 29 19 BsiHKAI G_wGCw'C Cuts at: 2572 3747 3832 4903 2572 Size: 1175 85 1071 3355 Fragments arranged by size: 3355 1175 1071 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2241 2572 2853 3005 3747 3832 Size: 399 370 81 331 281 152 742 85 Cuts at: 3832 4903 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 331 281 152 85 81 BspEI T'CCGG_A Cuts at: 2247 5349 2247 Size: 3102 2584 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2899 3041 844 Size: 2055 142 3489 Fragments arranged by size: 3489 2055 142 BsrBI CCG'CTC Cuts at: 1394 1823 3573 1394 Size: 429 1750 3507 Fragments arranged by size: 3507 1750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4181 4355 65 Size: 1183 2933 174 1396 Fragments arranged by size: 2933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2426 2718 4330 4517 1700 Size: 110 616 292 1612 187 2869 Fragments arranged by size: 2869 1612 616 292 187 110 BsrGI T'GTAC_A Cuts at: 96 2832 96 Size: 2736 2950 BssHII G'CGCG_C Cuts at: 1925 2115 1925 Size: 190 5496 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 794 Size: 1241 4445 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 2498 2860 1184 Size: 1314 362 4010 Fragments arranged by size: 4010 1314 362 BstXI CCAn_nnnn'nTGG Cuts at: 2815 2815 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2226 2391 3016 3383 3768 3785 5237 5323 Size: 254 165 625 367 385 17 1452 86 Cuts at: 5323 5335 5681 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 625 385 367 346 254 165 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2020 2043 2145 2176 2595 2784 Size: 552 939 16 23 102 31 419 189 Cuts at: 2784 2814 513 Size: 30 3385 Fragments arranged by size: 3385 939 552 419 189 102 31 30 23 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2026 2065 487 Size: 683 56 800 39 4108 Fragments arranged by size: 4108 800 683 56 39 BtsI GCAGTG_nn' Cuts at: 2239 3202 3996 4016 2239 Size: 963 794 20 3909 Fragments arranged by size: 3909 963 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2076 3133 Size: 54 1425 50 275 18 216 30 1057 Cuts at: 3133 4022 5651 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1057 889 275 216 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3133 1537 Size: 275 234 1087 4090 Fragments arranged by size: 4090 1087 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 2689 3616 1353 Size: 1088 248 927 3423 Fragments arranged by size: 3423 1088 927 248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4273 4647 4793 387 Size: 1064 138 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2570 2570 Size: 5686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2308 2752 2813 2957 3749 4346 Size: 393 463 213 444 61 144 792 597 Cuts at: 4346 5133 5401 1239 Size: 787 268 1524 Fragments arranged by size: 1524 792 787 597 463 444 393 268 213 144 61 EcoO109I rG'GnC_Cy Cuts at: 2157 2932 2157 Size: 775 4911 EcoRI G'AATT_C Cuts at: 2663 2995 3373 2663 Size: 332 378 4976 Fragments arranged by size: 4976 378 332 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2731 4833 1678 Size: 1053 2102 2531 Fragments arranged by size: 2531 2102 1053 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373 Size: 32 135 32 2755 32 42 32 2626 Fragments arranged by size: 2755 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 2719 3011 2719 Size: 292 5394 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 10 Size: 54 1425 343 246 3618 Fragments arranged by size: 3618 1425 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2813 3644 4003 4162 518 Size: 648 1647 831 359 159 2042 Fragments arranged by size: 2042 1647 831 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2178 3102 3779 4927 5172 1606 Size: 416 156 924 677 1148 245 2120 Fragments arranged by size: 2120 1148 924 677 416 245 156 NaeI GCC'GGC Cuts at: 2428 2428 Size: 5686 NcoI C'CATG_G Cuts at: 513 1065 2004 2145 2784 2814 513 Size: 552 939 141 639 30 3385 Fragments arranged by size: 3385 939 639 552 141 30 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NgoMIV G'CCGG_C Cuts at: 2426 2426 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2962 3044 4589 1132 Size: 97 1733 82 1545 2229 Fragments arranged by size: 2229 1733 1545 97 82 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5686 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2932 2932 Size: 5686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PspOMI G'GGCC_C Cuts at: 2156 2156 Size: 5686 PspXI vC'TCGA_Gb Cuts at: 2731 2731 Size: 5686 PstI C_TGCA'G Cuts at: 830 2031 2449 2910 3038 830 Size: 1201 418 461 128 3478 Fragments arranged by size: 3478 1201 461 418 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SacI G_AGCT'C Cuts at: 2572 2572 Size: 5686 SacII CC_GC'GG Cuts at: 2023 2179 2023 Size: 156 5530 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 2910 3038 2910 Size: 128 5558 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2027 2395 2445 2710 2878 2906 3034 Size: 219 982 368 50 265 168 28 128 Cuts at: 3034 4169 4850 5041 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 982 681 368 265 219 191 168 128 50 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2669 2731 2743 2855 3790 4691 Size: 197 926 726 62 12 112 935 901 Cuts at: 4691 4953 820 Size: 262 1553 Fragments arranged by size: 1553 935 926 901 726 262 197 112 62 12 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 2962 3044 1132 Size: 1830 82 3774 Fragments arranged by size: 3774 1830 82 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 2451 2759 1517 Size: 934 308 4444 Fragments arranged by size: 4444 934 308 StyI C'CwwG_G Cuts at: 513 1065 2004 2145 2784 2814 3125 513 Size: 552 939 141 639 30 311 3074 Fragments arranged by size: 3074 939 639 552 311 141 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2354 3713 3730 3883 4068 4513 Size: 1255 624 150 1359 17 153 185 445 Cuts at: 4513 325 Size: 1498 Fragments arranged by size: 1498 1359 1255 624 445 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2832 3932 Size: 274 80 33 51 1443 129 726 1100 Cuts at: 3932 5644 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1100 726 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 2143 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 2731 1943 Size: 788 4898 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI