################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeI MaeIII MamI MfeI MroI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Tsp509I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 906 GTAC GT^AC AflIII 791 ACGCGT A^C[GA][CT]GT ApaI 1157 GGGCCC GGGCC^C AspI 828 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 380 TGGCCA TGG^CCA BglII 1146 AGATCT A^GATCT BsaWI 226 ACCGGA [AT]^CCGG[AT] BsrBI 262 GAGCGG CCGCTC(-3/-3) BssHII 220 GCGCGC G^CGCGC BssSI 304 CACGAG CACGAG(-5/-1) BstAPI 303 GCACGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 384 CATCC GGATG(2/0) BstXI 859 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 828 GACGCAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 630 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 384 CATCC GGATG(9/13) KspI 144 CCGCGG CCGC^GG MaeII 91 ACGT A^CGT MluI 791 ACGCGT A^CGCGT MluNI 380 TGGCCA TGG^CCA MscI 380 TGGCCA TGG^CCA NspI 278 GCATGC [GA]CATG^[CT] PflFI 828 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 469 GGGTCCC [GA]G^G[AT]CC[CT] RsaI 906 GTAC GT^AC SacII 144 CCGCGG CCGC^GG SfaNI 1169 GCATC GCATC(5/9) SmaI 1003 CCCGGG CCC^GGG SphI 278 GCATGC GCATG^C StuI 1107 AGGCCT AGG^CCT TaqI 365 TCGA T^CGA TfiI 112 GATTC G^A[AT]TC Tth111I 828 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 365 TCGA T^CGA XcmI 856 CCACCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1003 CCCGGG C^CCGGG XmaI 1003 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AhdI 85 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 463 GACGCGGGGTC AspEI 85 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 463 GACGCGGGGTC AspHI 950 GAGCTC G[AT]GC[AT]^C 1101 GAGCTC AviII 275 TGCGCA TGC^GCA 281 TGCGCA BamHI 649 GGATCC G^GATCC 717 GGATCC BglI 26 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 187 GCCGCAGAGGC BsgI 301 CTGCAC GTGCAG(16/14) 1025 GTGCAG BsiHKAI 950 GAGCTC G[AT]GC[AT]^C 1101 GAGCTC BsmBI 794 CGTCTC CGTCTC(1/5) 367 GAGACG DraII 469 GGGTCCC [GA]G^G[ACGT]CC[CT] 1156 GGGGCCC DsaI 144 CCGCGG C^C[GA][CT]GG 501 CCGTGG EagI 341 CGGCCG C^GGCCG 407 CGGCCG Eam1105I 85 GACTCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 463 GACGCGGGGTC EclXI 341 CGGCCG C^GGCCG 407 CGGCCG Eco52I 341 CGGCCG C^GGCCG 407 CGGCCG EcoO109I 469 GGGTCCC [GA]G^G[ACGT]CC[CT] 1156 GGGGCCC FseI 72 GGCCGGCC GGCCGG^CC 342 GGCCGGCC FspI 275 TGCGCA TGC^GCA 281 TGCGCA NlaIII 279 CATG CATG^ 977 CATG SacI 950 GAGCTC GAGCT^C 1101 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIV PleI PstI Sau3AI Sau96I ScrFI SfcI SfoI StyI TseI TspRI XhoII