(Circular) (Six-base) MAPSORT of: pFN21AB0944.seq Check: 3997 from: 1 to: 5884 pFN21AB0944 5884 bp With 182 enzymes: SgfI * March 28, 2012 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3947 5331 5599 1632 Size: 2315 1384 268 1917 Fragments arranged by size: 2315 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 2796 4783 5760 1203 Size: 1593 1987 977 1327 Fragments arranged by size: 1987 1593 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 2095 2473 4613 2095 Size: 378 2140 3366 Fragments arranged by size: 3366 2140 378 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5884 AlwNI CAG_nnn'CTG Cuts at: 2359 2992 3129 3627 5199 2359 Size: 633 137 498 1572 3044 Fragments arranged by size: 3044 1572 633 498 137 ApaI G_GGCC'C Cuts at: 3166 3166 Size: 5884 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 3193 3419 3571 5680 1493 Size: 1700 226 152 2109 1697 Fragments arranged by size: 2109 1700 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2187 2690 3008 3209 1870 Size: 73 244 503 318 201 4545 Fragments arranged by size: 4545 503 318 244 201 73 BamHI G'GATC_C Cuts at: 2654 2722 3214 3581 2654 Size: 68 492 367 4957 Fragments arranged by size: 4957 492 367 68 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2341 3040 3068 3205 Size: 325 217 627 357 197 699 28 137 Cuts at: 3205 4660 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 699 627 357 325 217 197 137 28 BanII G_rGCy'C Cuts at: 1391 2680 2853 2940 2959 3110 3166 3203 1391 Size: 1289 173 87 19 151 56 37 4072 Fragments arranged by size: 4072 1289 173 151 87 56 37 19 BbeI G_GCGC'C Cuts at: 2148 2345 3072 2148 Size: 197 727 4960 Fragments arranged by size: 4960 727 197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4913 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4075 4109 1903 Size: 34 2138 34 3678 Fragments arranged by size: 3678 2138 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 2198 4495 Size: 107 122 71 631 605 365 161 2297 Cuts at: 4495 136 Size: 1525 Fragments arranged by size: 2297 1525 631 605 365 161 122 107 71 BglII A'GATC_T Cuts at: 3151 5879 3151 Size: 2728 3156 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2135 2501 2871 3045 3166 3945 5101 1790 Size: 345 366 370 174 121 779 1156 2573 Fragments arranged by size: 2573 1156 779 370 366 345 174 121 BmgBI CAC'GTC Cuts at: 1318 2097 1318 Size: 779 5105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2005 2095 3615 Size: 416 53 83 399 24 588 90 1520 Cuts at: 3615 4573 442 Size: 958 1753 Fragments arranged by size: 1753 1520 958 588 416 399 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2916 3040 3176 4544 1239 Size: 1677 124 136 1368 2579 Fragments arranged by size: 2579 1677 1368 136 124 Bpu10I CC'TnA_GC Cuts at: 3109 3109 Size: 5884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4009 4874 5172 4009 Size: 865 298 4721 Fragments arranged by size: 4721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2429 2629 2924 4547 882 Size: 617 930 200 295 1623 2219 Fragments arranged by size: 2219 1623 930 617 295 200 BsaAI yAC'GTr Cuts at: 493 1204 3675 493 Size: 711 2471 2702 Fragments arranged by size: 2702 2471 711 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2213 2342 2645 Size: 53 83 186 936 612 68 129 303 Cuts at: 2645 2810 3069 3691 4073 4708 275 Size: 165 259 622 382 635 1451 Fragments arranged by size: 1451 936 635 622 612 382 303 259 186 165 129 83 68 53 BsaWI w'CCGG_w Cuts at: 2231 4317 4715 4989 5136 5547 2231 Size: 2086 398 274 147 411 2568 Fragments arranged by size: 2568 2086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 3013 3043 1178 Size: 30 165 30 672 30 908 30 4019 Fragments arranged by size: 4019 908 672 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2010 2024 2098 2101 2447 1186 Size: 824 14 74 3 346 4623 Fragments arranged by size: 4623 824 346 74 14 3 BseYI C'CCAG_C Cuts at: 2067 2073 2419 2884 2950 5087 5583 2067 Size: 6 346 465 66 2137 496 2368 Fragments arranged by size: 2368 2137 496 465 346 66 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2291 3051 1163 Size: 573 555 760 3996 Fragments arranged by size: 3996 760 573 555 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2349 2415 2506 3334 4095 5123 Size: 275 186 348 66 91 828 761 1028 Cuts at: 5123 1540 Size: 2301 Fragments arranged by size: 2301 1028 828 761 348 275 186 91 66 BsiHKAI G_wGCw'C Cuts at: 2959 3110 3945 4030 5101 2959 Size: 151 835 85 1071 3742 Fragments arranged by size: 3742 1071 835 151 85 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 BsmBI CGTCTCn'nnnn_ Cuts at: 2366 2805 2366 Size: 439 5445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2501 2680 2853 2871 2940 2959 Size: 399 345 366 179 173 18 69 19 Cuts at: 2959 3045 3110 3166 3203 3945 4030 5101 1391 Size: 86 65 56 37 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 345 179 173 86 85 69 65 56 37 19 18 BspEI T'CCGG_A Cuts at: 5547 5547 Size: 5884 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3239 844 Size: 2395 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2269 3771 1394 Size: 429 446 1502 3507 Fragments arranged by size: 3507 1502 446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4379 4553 65 Size: 1183 3131 174 1396 Fragments arranged by size: 3131 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 2344 2348 2448 4528 4715 1700 Size: 110 268 266 4 100 2080 187 2869 Fragments arranged by size: 2869 2080 268 266 187 110 100 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 2225 1925 Size: 300 5584 BssSI C'ACGA_G Cuts at: 2309 3944 4956 2309 Size: 1635 1012 3237 Fragments arranged by size: 3237 1635 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 794 Size: 1520 4364 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstXI CCAn_nnnn'nTGG Cuts at: 2871 2871 Size: 5884 BstYI r'GATC_y Cuts at: 1972 2052 2654 2722 3151 3214 3581 3966 3983 Size: 80 602 68 429 63 367 385 17 Cuts at: 3983 5435 5521 5533 5879 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 602 429 385 367 346 86 80 68 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2149 2506 513 Size: 552 939 145 357 3891 Fragments arranged by size: 3891 939 552 357 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5145 Fragments arranged by size: 5145 683 56 BtsI GCAGTG_nn' Cuts at: 2450 2913 3400 4194 4214 2450 Size: 463 487 794 20 4120 Fragments arranged by size: 4120 794 487 463 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2839 4891 809 Size: 507 1523 2052 1802 Fragments arranged by size: 2052 1802 1523 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2080 2346 Size: 54 1425 50 275 18 208 42 266 Cuts at: 2346 2350 2385 2401 2412 2901 3331 4220 5849 Size: 4 35 16 11 489 430 889 1629 Cuts at: 5849 8 Size: 43 Fragments arranged by size: 1629 1425 889 489 430 275 266 208 54 50 43 42 35 18 16 11 4 EagI C'GGCC_G Cuts at: 1537 1812 2346 2412 3331 1537 Size: 275 534 66 919 4090 Fragments arranged by size: 4090 919 534 275 66 EarI CTCTTCn'nnn_ Cuts at: 1353 3814 1353 Size: 2461 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2259 2549 2617 2653 4471 4845 Size: 1064 138 670 290 68 36 1818 374 Cuts at: 4845 4991 387 Size: 146 1280 Fragments arranged by size: 1818 1280 1064 670 374 290 146 138 68 36 EcoICRI GAG'CTC Cuts at: 2957 3108 2957 Size: 151 5733 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2916 3040 3176 3947 4544 5331 5599 Size: 393 1284 124 136 771 597 787 268 Cuts at: 5599 1239 Size: 1524 Fragments arranged by size: 1524 1284 787 771 597 393 268 136 124 EcoNI CCTnn'n_nnAGG Cuts at: 2639 2639 Size: 5884 EcoO109I rG'GnC_Cy Cuts at: 2475 3162 2475 Size: 687 5197 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FseI GG_CCGG'CC Cuts at: 2082 2352 2082 Size: 270 5614 FspI TGC'GCA Cuts at: 2282 2288 4390 2282 Size: 6 2102 3776 Fragments arranged by size: 3776 2102 6 FspAI rTGC'GCAy Cuts at: 2288 2288 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 2148 2262 2345 2698 3072 5031 1678 Size: 470 114 83 353 374 1959 2531 Fragments arranged by size: 2531 1959 470 374 353 114 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2459 2491 4525 Size: 32 135 32 509 32 346 32 2034 Cuts at: 4525 4557 4599 4631 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2034 509 346 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3228 3474 669 Size: 988 281 1290 246 3079 Fragments arranged by size: 3079 1290 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KasI G'GCGC_C Cuts at: 2144 2341 3068 2144 Size: 197 727 4960 Fragments arranged by size: 4960 727 197 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 2796 5760 2796 Size: 2964 2920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2817 4998 5182 1305 Size: 1512 2181 184 2007 Fragments arranged by size: 2181 2007 1512 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 10 Size: 54 1425 343 555 3507 Fragments arranged by size: 3507 1425 555 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3842 4201 4360 518 Size: 648 2676 359 159 2042 Fragments arranged by size: 2676 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2151 2240 2251 2494 3300 3977 5125 5370 Size: 545 89 11 243 806 677 1148 245 Cuts at: 5370 1606 Size: 2120 Fragments arranged by size: 2120 1148 806 677 545 245 243 89 11 NaeI GCC'GGC Cuts at: 2080 2346 2350 2450 2080 Size: 266 4 100 5514 Fragments arranged by size: 5514 266 100 4 NarI GG'CG_CC Cuts at: 2145 2342 3069 2145 Size: 197 727 4960 Fragments arranged by size: 4960 727 197 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4393 Fragments arranged by size: 4393 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NgoMIV G'CCGG_C Cuts at: 2078 2344 2348 2448 2078 Size: 266 4 100 5514 Fragments arranged by size: 5514 266 100 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2287 3242 4787 1132 Size: 97 1058 955 1545 2229 Fragments arranged by size: 2229 1545 1058 955 97 PasI CC'CwG_GG Cuts at: 1348 2062 2727 1348 Size: 714 665 4505 Fragments arranged by size: 4505 714 665 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5884 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2475 2475 Size: 5884 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspOMI G'GGCC_C Cuts at: 3162 3162 Size: 5884 PstI C_TGCA'G Cuts at: 830 2183 2445 2997 3236 830 Size: 1353 262 552 239 3478 Fragments arranged by size: 3478 1353 552 262 239 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 SacI G_AGCT'C Cuts at: 2959 3110 2959 Size: 151 5733 SacII CC_GC'GG Cuts at: 2152 2152 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SanDI GG'GwC_CC Cuts at: 2475 2475 Size: 5884 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2179 2441 2993 3232 4367 5048 5239 Size: 219 1134 262 552 239 1135 681 191 Cuts at: 5239 826 Size: 1471 Fragments arranged by size: 1471 1135 1134 681 552 262 239 219 191 SfoI GGC'GCC Cuts at: 2146 2343 3070 2146 Size: 197 727 4960 Fragments arranged by size: 4960 727 197 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 3010 3211 1872 Size: 1138 201 4545 Fragments arranged by size: 4545 1138 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 3988 4889 5151 820 Size: 197 926 2045 901 262 1553 Fragments arranged by size: 2045 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 2287 3242 1132 Size: 1155 955 3774 Fragments arranged by size: 3774 1155 955 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 3114 1517 Size: 1597 4287 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2480 2779 2818 3323 513 Size: 552 939 333 143 299 39 505 3074 Fragments arranged by size: 3074 939 552 505 333 299 143 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2991 3911 3928 4081 4266 4711 325 Size: 1255 1411 920 17 153 185 445 1498 Fragments arranged by size: 1498 1411 1255 920 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 3013 3045 5844 5876 2075 Size: 32 906 32 2799 32 2083 Fragments arranged by size: 2799 2083 906 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2836 1316 Size: 1520 4364 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2868 2868 Size: 5884 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 3008 3209 1870 Size: 1138 201 4545 Fragments arranged by size: 4545 1138 201 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI