(Circular) (Six-base) MAPSORT of: pFN21AB4674.seq Check: 899 from: 1 to: 5881 pFN21AB4674 5881 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2143 2143 Size: 5881 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2625 3944 5328 5596 1632 Size: 993 1319 1384 268 1917 Fragments arranged by size: 1917 1384 1319 993 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2060 3624 5196 2060 Size: 1564 1572 2745 Fragments arranged by size: 2745 1572 1564 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2442 3190 3416 3568 5677 1493 Size: 949 748 226 152 2109 1697 Fragments arranged by size: 2109 1697 949 748 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2190 3206 1870 Size: 73 247 1016 4545 Fragments arranged by size: 4545 1016 247 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3202 4657 618 Size: 325 217 627 1415 1455 1842 Fragments arranged by size: 1842 1455 1415 627 325 217 BanII G_rGCy'C Cuts at: 1391 2190 2584 3200 1391 Size: 799 394 616 4072 Fragments arranged by size: 4072 799 616 394 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BbvCI CC'TCA_GC Cuts at: 2061 2061 Size: 5881 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2057 2091 4072 4106 1903 Size: 34 120 34 1981 34 3678 Fragments arranged by size: 3678 1981 120 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 2476 2961 3171 1548 Size: 928 485 210 4258 Fragments arranged by size: 4258 928 485 210 BfrBI ATG'CAT Cuts at: 2688 2688 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4492 136 Size: 107 122 71 631 605 2820 1525 Fragments arranged by size: 2820 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 2070 2840 3942 5098 1790 Size: 280 770 1102 1156 2573 Fragments arranged by size: 2573 1156 1102 770 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3612 4570 442 Size: 416 53 83 399 24 2195 958 1753 Fragments arranged by size: 2195 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 4541 1239 Size: 1203 2099 2579 Fragments arranged by size: 2579 2099 1203 Bpu10I CC'TnA_GC Cuts at: 2045 2061 2045 Size: 16 5865 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 2700 5875 2700 Size: 3175 2706 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 3688 4070 4705 Size: 53 83 186 936 541 1614 382 635 Cuts at: 4705 275 Size: 1451 Fragments arranged by size: 1614 1451 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2182 2550 4314 4712 4986 5133 5544 2182 Size: 368 1764 398 274 147 411 2519 Fragments arranged by size: 2519 1764 411 398 368 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5656 Fragments arranged by size: 5656 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5881 BseYI C'CCAG_C Cuts at: 5084 5580 5084 Size: 496 5385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3942 4027 5098 3942 Size: 85 1071 4725 Fragments arranged by size: 4725 1071 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2190 2584 2840 3200 3942 4027 Size: 399 280 120 394 256 360 742 85 Cuts at: 4027 5098 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 360 280 256 120 85 BspEI T'CCGG_A Cuts at: 2182 5544 2182 Size: 3362 2519 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2143 3236 844 Size: 1299 1093 3489 Fragments arranged by size: 3489 1299 1093 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2433 2620 4376 4550 65 Size: 1183 1185 187 1756 174 1396 Fragments arranged by size: 1756 1396 1185 1183 187 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 4525 4712 1700 Size: 110 217 2498 187 2869 Fragments arranged by size: 2869 2498 217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 2990 3941 4953 2990 Size: 951 1012 3918 Fragments arranged by size: 3918 1012 951 BstAPI GCAn_nnn'nTGC Cuts at: 794 2615 794 Size: 1821 4060 BstBI TT'CG_AA Cuts at: 3163 4685 3163 Size: 1522 4359 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstXI CCAn_nnnn'nTGG Cuts at: 3141 3141 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2179 2266 2521 3211 3578 3963 3980 5432 Size: 207 87 255 690 367 385 17 1452 Cuts at: 5432 5518 5530 5876 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 690 385 367 346 255 207 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2871 513 Size: 552 939 867 3523 Fragments arranged by size: 3523 939 867 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 3397 4191 4211 3397 Size: 794 20 5067 Fragments arranged by size: 5067 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 3328 4217 Size: 54 1425 50 275 18 429 1069 889 Cuts at: 4217 5846 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1069 889 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3811 1353 Size: 2458 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4468 4842 4988 387 Size: 1064 138 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2442 2625 3944 4541 5328 5596 1239 Size: 393 810 183 1319 597 787 268 1524 Fragments arranged by size: 1524 1319 810 787 597 393 268 183 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2693 2903 2693 Size: 210 5671 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 2967 2999 4522 Size: 32 135 32 816 32 547 32 1523 Cuts at: 4522 4554 4596 4628 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1523 816 547 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 2885 748 Size: 2137 3744 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2374 4995 5179 1305 Size: 1069 2621 184 2007 Fragments arranged by size: 2621 2007 1069 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 10 Size: 54 1425 343 429 3630 Fragments arranged by size: 3630 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3839 4198 4357 518 Size: 648 2673 359 159 2042 Fragments arranged by size: 2673 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2747 3297 3974 5122 5367 1606 Size: 1141 550 677 1148 245 2120 Fragments arranged by size: 2120 1148 1141 677 550 245 NaeI GCC'GGC Cuts at: 2029 2029 Size: 5881 NcoI C'CATG_G Cuts at: 513 1065 2004 2871 513 Size: 552 939 867 3523 Fragments arranged by size: 3523 939 867 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NgoMIV G'CCGG_C Cuts at: 2027 2027 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NsiI A_TGCA'T Cuts at: 2690 2690 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 3239 4784 1132 Size: 97 2010 1545 2229 Fragments arranged by size: 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2712 2744 2712 Size: 32 5849 PstI C_TGCA'G Cuts at: 830 2278 3233 830 Size: 1448 955 3478 Fragments arranged by size: 3478 1448 955 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2274 2760 3033 3229 4364 5045 5236 Size: 219 1229 486 273 196 1135 681 191 Cuts at: 5236 826 Size: 1471 Fragments arranged by size: 1471 1229 1135 681 486 273 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 2192 3208 1872 Size: 320 1016 4545 Fragments arranged by size: 4545 1016 320 SmlI C'TyrA_G Cuts at: 820 1017 1943 3985 4886 5148 820 Size: 197 926 2042 901 262 1553 Fragments arranged by size: 2042 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 2283 3805 5 Size: 47 2231 1522 2081 Fragments arranged by size: 2231 2081 1522 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2558 2871 2930 3320 513 Size: 552 939 240 314 313 59 390 3074 Fragments arranged by size: 3074 939 552 390 314 313 240 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3908 3925 4078 4263 4708 325 Size: 1255 2328 17 153 185 445 1498 Fragments arranged by size: 2328 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4127 5839 96 Size: 274 80 33 51 1443 2150 1712 138 Fragments arranged by size: 2150 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 3088 3120 5841 5873 2622 Size: 32 434 32 2721 32 2630 Fragments arranged by size: 2721 2630 434 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 2190 3206 1870 Size: 320 1016 4545 Fragments arranged by size: 4545 1016 320 XmnI GAAnn'nnTTC Cuts at: 2222 4010 2222 Size: 1788 4093 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI