Restriction map for
pFN21AB4651
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HindIII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 137 GTCTAC GT^[AC][GT]AC
AciI 798 CCGC CCGC(-3/-1)
AfaI 176 GTAC GT^AC
AlwNI 22 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 635 GAGCCC G[GA]GC[CT]^C
BbvCI 583 GCTGAGG CCTCAGC(-5/-2)
BglI 181 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 635 GAGCCC G[AGT]GC[ACT]^C
BsaI 90 GAGACC GGTCTC(1/5)
BsaJI 550 CCAGGG C^C[ACGT][ACGT]GG
BsgI 102 GTGCAG GTGCAG(16/14)
Bsp1286I 635 GAGCCC G[AGT]GC[ACT]^C
BsrDI 203 GCAATG GCAATG(2/0)
BsrI 241 CCAGT ACTGG(1/-1)
CfoI 507 GCGC GCG^C
EcoRI 575 GAATTC G^AATTC
HapII 4 CCGG C^CGG
HhaI 507 GCGC GCG^C
HinP1I 507 GCGC G^CGC
HincII 691 GTTAAC GT[CT]^[GA]AC
HindII 691 GTTAAC GT[CT]^[GA]AC
HpaI 691 GTTAAC GTT^AAC
HpaII 4 CCGG C^CGG
MaeII 251 ACGT A^CGT
MslI 95 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 4 CCGG C^CGG
NspI 190 GCATGT [GA]CATG^[CT]
PstI 299 CTGCAG CTGCA^G
RsaI 176 GTAC GT^AC
SspI 779 AATATT AAT^ATT
StuI 131 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaII 6 GGACC G^G[AT]CC
238 GGTCC
BsiYI 229 CCAAAATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
550 CCAGGGATAGG
BslI 229 CCAAAATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
550 CCAGGGATAGG
BsmAI 90 GAGAC GTCTC(1/5)
610 GTCTC
BspMI 98 GCAGGT ACCTGC(4/8)
366 GCAGGT
BssKI 129 CCAGG ^CC[ACGT]GG
550 CCAGG
BstF5I 153 GGATG GGATG(2/0)
393 GGATG
BstNI 129 CCAGG CC^[AT]GG
550 CCAGG
BstYI 109 GGATCT [GA]^GATC[CT]
258 GGATCT
Cfr13I 6 GGACC G^G[ACGT]CC
238 GGTCC
DraI 534 TTTAAA TTT^AAA
754 TTTAAA
EcoRII 129 CCAGG ^CC[AT]GG
550 CCAGG
FokI 153 GGATG GGATG(9/13)
393 GGATG
HaeIII 132 GGCC GG^CC
588 GGCC
HinfI 474 GATTC G^A[ACGT]TC
623 GAATC
HphI 87 GGTGA GGTGA(8/7)
126 TCACC
MaeIII 340 GTGAC ^GT[ACGT]AC
355 GTGAC
MboII 64 GAAGA GAAGA(8/7)
826 GAAGA
MflI 109 GGATCT [GA]^GATC[CT]
258 GGATCT
MvaI 129 CCAGG CC^[AT]GG
550 CCAGG
MwoI 181 GCCAGAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
730 GCGTTTGTTGC
NlaIV 237 GGGTCC GG[ACGT]^[ACGT]CC
634 GGAGCC
Sau96I 6 GGACC G^G[ACGT]CC
238 GGTCC
ScrFI 129 CCAGG CC^[ACGT]GG
550 CCAGG
SfaNI 24 GATGC GCATC(5/9)
363 GATGC
SfcI 16 CTACAG C^T[GA][CT]AG
299 CTGCAG
TfiI 474 GATTC G^A[AT]TC
623 GAATC
Tsp45I 340 GTGAC ^GT[GC]AC
355 GTGAC
XhoII 109 GGATCT [GA]^GATC[CT]
258 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
DdeI DpnII Fnu4HI ItaI MboI
MnlI MseI MspA1I NdeII NlaIII
PvuII Sau3AI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I