(Circular) (Six-base) MAPSORT of: pFN21AE9636.seq Check: 3966 from: 1 to: 9382 pFN21AE9636 9382 bp With 182 enzymes: SgfI * March 28, 2012 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4762 5055 5566 4762 Size: 293 511 8578 Fragments arranged by size: 8578 511 293 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7192 278 Size: 53 83 186 936 5656 2468 Fragments arranged by size: 5656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4039 4253 4537 6725 1937 Size: 2102 214 284 2188 4594 Fragments arranged by size: 4594 2188 2102 284 214 Acc65I G'GTAC_C Cuts at: 6703 6703 Size: 9382 AclI AA'CG_TT Cuts at: 1563 7509 7882 1563 Size: 5946 373 3063 Fragments arranged by size: 5946 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 4310 4419 5916 6585 7445 8829 9097 Size: 544 2134 109 1497 669 860 1384 268 Cuts at: 9097 1632 Size: 1917 Fragments arranged by size: 2134 1917 1497 1384 860 669 544 268 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9185 AflIII A'CryG_T Cuts at: 1203 2534 4139 8281 9258 1203 Size: 1331 1605 4142 977 1327 Fragments arranged by size: 4142 1605 1331 1327 977 AgeI A'CCGG_T Cuts at: 8213 8213 Size: 9382 AhdI GACnn_n'nnGTC Cuts at: 4862 8111 4862 Size: 3249 6133 AleI CACnn'nnGTG Cuts at: 1166 2041 2813 3585 1166 Size: 875 772 772 6963 Fragments arranged by size: 6963 875 772 772 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3512 4489 6047 1184 Size: 2328 977 1558 4519 Fragments arranged by size: 4519 2328 1558 977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3257 3289 3257 Size: 32 9350 AlwNI CAG_nnn'CTG Cuts at: 2218 4076 4240 4711 4927 5227 5435 5581 5803 Size: 1858 164 471 216 300 208 146 222 Cuts at: 5803 5887 5993 6268 6467 7125 8697 2218 Size: 84 106 275 199 658 1572 2903 Fragments arranged by size: 2903 1858 1572 658 471 300 275 222 216 208 199 164 146 106 84 ApaI G_GGCC'C Cuts at: 6193 6364 6521 6193 Size: 171 157 9054 Fragments arranged by size: 9054 171 157 ApaLI G'TGCA_C Cuts at: 7439 8595 7439 Size: 1156 8226 ApoI r'AATT_y Cuts at: 1493 2276 3344 5278 5723 6691 6917 7069 9178 Size: 783 1068 1934 445 968 226 152 2109 Cuts at: 9178 1493 Size: 1697 Fragments arranged by size: 2109 1934 1697 1068 968 783 445 226 152 AseI AT'TA_AT Cuts at: 160 1032 7936 160 Size: 872 6904 1606 Fragments arranged by size: 6904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9382 AvaI C'yCGr_G Cuts at: 1870 1943 2409 2994 3168 4482 5088 5819 6186 Size: 73 466 585 174 1314 606 731 367 Cuts at: 6186 6227 6351 6420 6707 1870 Size: 41 124 69 287 4545 Fragments arranged by size: 4545 1314 731 606 585 466 367 287 174 124 73 69 41 BamHI G'GATC_C Cuts at: 6300 6712 7079 6300 Size: 412 367 8603 Fragments arranged by size: 8603 412 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3102 3247 3381 3496 3610 Size: 325 217 627 1315 145 134 115 114 Cuts at: 3610 3879 4744 4835 4871 5754 6703 8158 618 Size: 269 865 91 36 883 949 1455 1842 Fragments arranged by size: 1842 1455 1315 949 883 865 627 325 269 217 145 134 115 114 91 36 BanII G_rGCy'C Cuts at: 1391 2297 2410 4489 4560 5599 5934 6107 6193 Size: 906 113 2079 71 1039 335 173 86 Cuts at: 6193 6207 6352 6364 6455 6521 6701 1391 Size: 14 145 12 91 66 180 4072 Fragments arranged by size: 4072 2079 1039 906 335 180 173 145 113 91 86 71 66 14 12 BbeI G_GCGC'C Cuts at: 3883 5758 3883 Size: 1875 7507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2682 3514 3602 3916 928 Size: 971 783 832 88 314 6394 Fragments arranged by size: 6394 971 832 783 314 88 BbvCI CC'TCA_GC Cuts at: 4656 4822 5660 6468 6669 4656 Size: 166 838 808 201 7369 Fragments arranged by size: 7369 838 808 201 166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2106 2140 5477 5511 7573 7607 1903 Size: 34 169 34 3337 34 2062 34 3678 Fragments arranged by size: 3678 3337 2062 169 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5492 7274 8484 5492 Size: 1782 1210 6390 Fragments arranged by size: 6390 1782 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9382 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3877 4179 4652 Size: 107 122 71 631 605 2205 302 473 Cuts at: 4652 5812 5882 7993 136 Size: 1160 70 2111 1525 Fragments arranged by size: 2205 2111 1525 1160 631 605 473 302 122 107 71 70 BglII A'GATC_T Cuts at: 2712 6118 9377 2712 Size: 3406 3259 2717 Fragments arranged by size: 3406 3259 2717 BlpI GC'TnA_GC Cuts at: 4077 5928 6799 4077 Size: 1851 871 6660 Fragments arranged by size: 6660 1851 871 Bme1580I G_kGCm'C Cuts at: 1790 2048 3107 3252 3501 3615 4390 4838 6193 Size: 258 1059 145 249 114 775 448 1355 Cuts at: 6193 6364 6521 7443 8599 1790 Size: 171 157 922 1156 2573 Fragments arranged by size: 2573 1355 1156 1059 922 775 448 258 249 171 157 145 114 BmgBI CAC'GTC Cuts at: 1318 2537 1318 Size: 1219 8163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2953 3592 5511 Size: 416 53 83 399 24 1536 639 1919 Cuts at: 5511 6516 7113 8071 442 Size: 1005 597 958 1753 Fragments arranged by size: 1919 1753 1536 1005 958 639 597 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9382 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5272 6121 6366 5272 Size: 849 245 8288 Fragments arranged by size: 8288 849 245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2532 2897 3298 3577 4076 4207 4364 4672 Size: 1293 365 401 279 499 131 157 308 Cuts at: 4672 4748 4994 5216 5263 5318 5786 6074 6143 Size: 76 246 222 47 55 468 288 69 Cuts at: 6143 6314 6335 6341 6386 6515 8042 1239 Size: 171 21 6 45 129 1527 2579 Fragments arranged by size: 2579 1527 1293 499 468 401 365 308 288 279 246 222 171 157 131 129 76 69 55 47 45 21 6 Bpu10I CC'TnA_GC Cuts at: 4656 4822 5082 5660 6106 6454 6468 6669 4656 Size: 166 260 578 446 348 14 201 7369 Fragments arranged by size: 7369 578 446 348 260 201 166 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5027 5051 5255 5734 7507 8372 8670 5027 Size: 24 204 479 1773 865 298 5739 Fragments arranged by size: 5739 1773 865 479 298 204 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2073 5762 8045 882 Size: 617 574 3689 2283 2219 Fragments arranged by size: 3689 2283 2219 617 574 BsaAI yAC'GTr Cuts at: 493 1204 3511 4140 7173 493 Size: 711 2307 629 3033 2702 Fragments arranged by size: 3033 2702 2307 711 629 BsaBI GATnn'nnATC Cuts at: 4013 9376 4013 Size: 5363 4019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4036 5755 7189 Size: 53 83 186 936 2347 156 1719 1434 Cuts at: 7189 7571 8206 275 Size: 382 635 1451 Fragments arranged by size: 2347 1719 1451 1434 936 635 382 186 156 83 53 BsaWI w'CCGG_w Cuts at: 2038 2659 3909 7815 8213 8487 8634 9045 2038 Size: 621 1250 3906 398 274 147 411 2375 Fragments arranged by size: 3906 2375 1250 621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 3257 3287 3765 Size: 30 165 30 597 30 1227 30 478 Cuts at: 3765 3795 5357 5387 6054 6084 6116 6146 6616 Size: 30 1562 30 667 30 32 30 470 Cuts at: 6616 6646 1178 Size: 30 3914 Fragments arranged by size: 3914 1562 1227 667 597 478 470 165 32 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2287 2567 2840 2903 3060 3413 3416 3950 Size: 1101 280 273 63 157 353 3 534 Cuts at: 3950 4967 5213 5536 5648 5981 6113 6215 6440 Size: 1017 246 323 112 333 132 102 225 Cuts at: 6440 6485 6497 6512 6515 1186 Size: 45 12 15 3 4053 Fragments arranged by size: 4053 1101 1017 534 353 333 323 280 273 246 225 157 132 112 102 63 45 15 12 3 3 BseYI C'CCAG_C Cuts at: 2064 2418 3875 4732 4755 4947 5368 5398 5585 Size: 354 1457 857 23 192 421 30 187 Cuts at: 5585 6415 6551 8585 9081 2064 Size: 830 136 2034 496 2365 Fragments arranged by size: 2365 2034 1457 857 830 496 421 354 192 187 136 30 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 2899 3677 3789 5337 6510 1163 Size: 573 888 275 778 112 1548 1173 4035 Fragments arranged by size: 4035 1548 1173 888 778 573 275 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6832 7593 8621 1540 Size: 275 186 4831 761 1028 2301 Fragments arranged by size: 4831 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3897 4223 4489 4560 5540 5599 5934 6207 6455 Size: 326 266 71 980 59 335 273 248 Cuts at: 6455 7443 7528 8599 3897 Size: 988 85 1071 4680 Fragments arranged by size: 4680 1071 988 980 335 326 273 266 248 85 71 59 BsmI GAATG_Cn' Cuts at: 6893 6986 6893 Size: 93 9289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2297 2410 3107 3252 3501 3615 Size: 399 258 249 113 697 145 249 114 Cuts at: 3615 3897 4223 4390 4489 4560 4838 5540 5599 Size: 282 326 167 99 71 278 702 59 Cuts at: 5599 5934 6107 6193 6207 6352 6364 6455 6521 Size: 335 173 86 14 145 12 91 66 Cuts at: 6521 6701 7443 7528 8599 1391 Size: 180 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 697 399 335 326 282 278 258 249 249 180 173 167 145 145 114 113 99 91 86 85 71 66 59 14 12 BspEI T'CCGG_A Cuts at: 2659 3909 9045 2659 Size: 1250 5136 2996 Fragments arranged by size: 5136 2996 1250 BspHI T'CATG_A Cuts at: 3711 6207 7271 9001 3711 Size: 2496 1064 1730 4092 Fragments arranged by size: 4092 2496 1730 1064 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4248 4596 4762 5055 5566 6231 6737 844 Size: 3404 348 166 293 511 665 506 3489 Fragments arranged by size: 3489 3404 665 511 506 348 293 166 BsrBI CCG'CTC Cuts at: 1394 1823 4033 4411 7269 1394 Size: 429 2210 378 2858 3507 Fragments arranged by size: 3507 2858 2210 429 378 BsrDI GCAATG_nn' Cuts at: 65 1248 2959 4935 7877 8051 65 Size: 1183 1711 1976 2942 174 1396 Fragments arranged by size: 2942 1976 1711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2631 3439 3903 4230 5324 5349 6583 Size: 110 821 808 464 327 1094 25 1234 Cuts at: 6583 8026 8213 1700 Size: 1443 187 2869 Fragments arranged by size: 2869 1443 1234 1094 821 808 464 327 187 110 25 BsrGI T'GTAC_A Cuts at: 96 5376 96 Size: 5280 4102 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9382 BssSI C'ACGA_G Cuts at: 4662 7442 8454 4662 Size: 2780 1012 5590 Fragments arranged by size: 5590 2780 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3247 4240 4927 5803 5993 6268 794 Size: 2453 993 687 876 190 275 3908 Fragments arranged by size: 3908 2453 993 876 687 275 190 BstBI TT'CG_AA Cuts at: 8186 8186 Size: 9382 BstEII G'GTnAC_C Cuts at: 1184 5126 6232 1184 Size: 3942 1106 4334 Fragments arranged by size: 4334 3942 1106 BstXI CCAn_nnnn'nTGG Cuts at: 2737 2815 4514 5524 2737 Size: 78 1699 1010 6595 Fragments arranged by size: 6595 1699 1010 78 BstYI r'GATC_y Cuts at: 1972 2139 2328 2712 3081 5115 6118 6300 6712 Size: 167 189 384 369 2034 1003 182 412 Cuts at: 6712 7079 7464 7481 8933 9019 9031 9377 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2034 1977 1452 1003 412 385 384 369 367 346 189 182 167 86 17 12 BstZ17I GTA'TAC Cuts at: 4254 4254 Size: 9382 Bsu36I CC'TnA_GG Cuts at: 4749 5420 5964 6036 6177 4749 Size: 671 544 72 141 7954 Fragments arranged by size: 7954 671 544 141 72 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 3736 5629 513 Size: 552 939 147 1585 1893 4266 Fragments arranged by size: 4266 1893 1585 939 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2080 2098 2844 4801 6423 487 Size: 683 56 854 18 746 1957 1622 3446 Fragments arranged by size: 3446 1957 1622 854 746 683 56 18 BtsI GCAGTG_nn' Cuts at: 3855 4679 5609 5693 5780 5843 6025 6898 7692 Size: 824 930 84 87 63 182 873 794 Cuts at: 7692 7712 3855 Size: 20 5525 Fragments arranged by size: 5525 930 873 824 794 182 87 84 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3241 3276 529 Size: 35 2677 35 6635 Fragments arranged by size: 6635 2677 35 35 DraI TTT'AAA Cuts at: 3214 6686 3214 Size: 3472 5910 DraIII CAC_nnn'GTG Cuts at: 4528 4528 Size: 9382 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4184 8389 809 Size: 507 2868 4205 1802 Fragments arranged by size: 4205 2868 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2629 2754 3201 Size: 54 1425 50 275 18 799 125 447 Cuts at: 3201 4504 5508 6829 7718 9347 8 Size: 1303 1004 1321 889 1629 43 Fragments arranged by size: 1629 1425 1321 1303 1004 889 799 447 275 125 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6829 1537 Size: 275 5017 4090 Fragments arranged by size: 5017 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3390 4023 6483 7312 1353 Size: 2037 633 2460 829 3423 Fragments arranged by size: 3423 2460 2037 829 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2132 2341 3137 4375 5924 6397 Size: 1064 138 543 209 796 1238 1549 473 Cuts at: 6397 6602 7969 8343 8489 387 Size: 205 1367 374 146 1280 Fragments arranged by size: 1549 1367 1280 1238 1064 796 543 473 374 209 205 146 138 EcoICRI GAG'CTC Cuts at: 4487 4558 5597 5932 6205 6453 4487 Size: 71 1039 335 273 248 7416 Fragments arranged by size: 7416 1039 335 273 248 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2532 2897 3298 3577 4076 4207 Size: 393 544 356 365 401 279 499 131 Cuts at: 4207 4310 4364 4419 4672 4748 4994 5216 5263 Size: 103 54 55 253 76 246 222 47 Cuts at: 5263 5318 5786 5916 6074 6143 6314 6335 6341 Size: 55 468 130 158 69 171 21 6 Cuts at: 6341 6386 6515 6585 7445 8042 8829 9097 1239 Size: 45 129 70 860 597 787 268 1524 Fragments arranged by size: 1524 860 787 597 544 499 468 401 393 365 356 279 268 253 246 222 171 158 131 130 129 103 76 70 69 55 55 54 47 45 21 6 EcoNI CCTnn'n_nnAGG Cuts at: 4460 5615 6011 6227 4460 Size: 1155 396 216 7615 Fragments arranged by size: 7615 1155 396 216 EcoO109I rG'GnC_Cy Cuts at: 2726 2792 4048 4669 5158 5179 5416 5425 5830 Size: 66 1256 621 489 21 237 9 405 Cuts at: 5830 6190 6220 6361 6517 6518 6605 2726 Size: 360 30 141 156 1 87 5503 Fragments arranged by size: 5503 1256 621 489 405 360 237 156 141 87 66 30 21 9 1 EcoRI G'AATT_C Cuts at: 6691 7069 6691 Size: 378 9004 EcoRV GAT'ATC Cuts at: 3481 3481 Size: 9382 FspI TGC'GCA Cuts at: 5412 7888 5412 Size: 2476 6906 HaeII r_GCGC'y Cuts at: 1678 2425 3883 4117 5758 8529 1678 Size: 747 1458 234 1641 2771 2531 Fragments arranged by size: 2771 2531 1641 1458 747 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2843 2875 2977 Size: 32 135 32 428 32 811 32 102 Cuts at: 2977 3009 3315 3347 3765 3797 3906 3938 4848 Size: 32 306 32 418 32 109 32 910 Cuts at: 4848 4880 5312 5344 5417 5449 5662 5694 6116 Size: 32 432 32 73 32 213 32 422 Cuts at: 6116 6148 8023 8055 8097 8129 1373 Size: 32 1875 32 42 32 2626 Fragments arranged by size: 2626 1875 910 811 432 428 422 418 306 213 135 109 102 73 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2856 3847 6153 6726 6972 669 Size: 988 281 918 991 2306 573 246 3079 Fragments arranged by size: 3079 2306 991 988 918 573 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9382 HpaI GTT'AAC Cuts at: 6972 6972 Size: 9382 KasI G'GCGC_C Cuts at: 3879 5754 3879 Size: 1875 7507 KpnI G_GTAC'C Cuts at: 6707 6707 Size: 9382 MfeI C'AATT_G Cuts at: 6981 6981 Size: 9382 MluI A'CGCG_T Cuts at: 9258 9258 Size: 9382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2043 3401 3426 4695 6147 8496 8680 1305 Size: 738 1358 25 1269 1452 2349 184 2007 Fragments arranged by size: 2349 2007 1452 1358 1269 738 184 25 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 10 Size: 54 1425 343 924 6636 Fragments arranged by size: 6636 1425 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2041 2813 2921 2952 3236 3471 3494 Size: 648 875 772 108 31 284 235 23 Cuts at: 3494 3585 3710 3728 4019 4203 7340 7699 7858 Size: 91 125 18 291 184 3137 359 159 Cuts at: 7858 518 Size: 2042 Fragments arranged by size: 3137 2042 875 772 648 359 291 284 235 184 159 125 108 91 31 23 18 MspA1I CmG'CkG Cuts at: 1606 2153 3354 3810 3995 4503 4732 4768 5491 Size: 547 1201 456 185 508 229 36 723 Cuts at: 5491 5862 6265 6798 7475 8623 8868 1606 Size: 371 403 533 677 1148 245 2120 Fragments arranged by size: 2120 1201 1148 723 677 547 533 508 456 403 371 245 229 185 36 NaeI GCC'GGC Cuts at: 2633 3905 4232 5326 2633 Size: 1272 327 1094 6689 Fragments arranged by size: 6689 1272 1094 327 NarI GG'CG_CC Cuts at: 3880 5755 3880 Size: 1875 7507 NcoI C'CATG_G Cuts at: 513 1065 2004 3736 5629 513 Size: 552 939 1732 1893 4266 Fragments arranged by size: 4266 1893 1732 939 552 NdeI CA'TA_TG Cuts at: 387 2974 3243 6257 387 Size: 2587 269 3014 3512 Fragments arranged by size: 3512 3014 2587 269 NgoMIV G'CCGG_C Cuts at: 2631 3903 4230 5324 2631 Size: 1272 327 1094 6689 Fragments arranged by size: 6689 1272 1094 327 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9382 NotI GC'GGCC_GC Cuts at: 6829 6829 Size: 9382 NruI TCG'CGA Cuts at: 2099 2099 Size: 9382 NspI r_CATG'y Cuts at: 1132 1229 3235 6740 8285 1132 Size: 97 2006 3505 1545 2229 Fragments arranged by size: 3505 2229 2006 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9382 PciI A'CATG_T Cuts at: 8281 8281 Size: 9382 PflMI CCAn_nnn'nTGG Cuts at: 1108 2280 3420 4528 5862 1108 Size: 1172 1140 1108 1334 4628 Fragments arranged by size: 4628 1334 1172 1140 1108 PfoI T'CCnGG_A Cuts at: 2874 5240 6477 2874 Size: 2366 1237 5779 Fragments arranged by size: 5779 2366 1237 PmeI GTTT'AAAC Cuts at: 6686 6686 Size: 9382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3257 3289 7451 7483 8964 8996 3257 Size: 32 4162 32 1481 32 3643 Fragments arranged by size: 4162 3643 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5179 5416 5425 6220 5179 Size: 237 9 795 8341 Fragments arranged by size: 8341 795 237 9 PshAI GACnn'nnGTC Cuts at: 1613 5613 1613 Size: 4000 5382 PsiI TTA'TAA Cuts at: 6952 6952 Size: 9382 PspOMI G'GGCC_C Cuts at: 6189 6360 6517 6189 Size: 171 157 9054 Fragments arranged by size: 9054 171 157 PspXI vC'TCGA_Gb Cuts at: 6227 6227 Size: 9382 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3441 3473 3441 Size: 32 9350 PstI C_TGCA'G Cuts at: 830 2652 3186 4440 4707 4923 5490 5604 5799 Size: 1822 534 1254 267 216 567 114 195 Cuts at: 5799 5838 5989 6273 6734 830 Size: 39 151 284 461 3478 Fragments arranged by size: 3478 1822 1254 567 534 461 284 267 216 195 151 114 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9382 PvuII CAG'CTG Cuts at: 3995 4732 5491 5862 6265 3995 Size: 737 759 371 403 7112 Fragments arranged by size: 7112 759 737 403 371 SacI G_AGCT'C Cuts at: 4489 4560 5599 5934 6207 6455 4489 Size: 71 1039 335 273 248 7416 Fragments arranged by size: 7416 1039 335 273 248 71 SacII CC_GC'GG Cuts at: 2154 2154 Size: 9382 SalI G'TCGA_C Cuts at: 1936 6724 1936 Size: 4788 4594 SanDI GG'GwC_CC Cuts at: 5425 5425 Size: 9382 SapI GCTCTTCn'nnn_ Cuts at: 4023 6483 4023 Size: 2460 6922 SbfI CC_TGCA'GG Cuts at: 6734 6734 Size: 9382 ScaI AGT'ACT Cuts at: 7630 9342 7630 Size: 1712 7670 SexAI A'CCwGG_T Cuts at: 3599 3599 Size: 9382 SfcI C'TryA_G Cuts at: 826 1045 2341 2648 2708 3182 4436 4538 4703 Size: 219 1296 307 60 474 1254 102 165 Cuts at: 4703 4919 5219 5486 5600 5795 5834 5945 5985 Size: 216 300 267 114 195 39 111 40 Cuts at: 5985 6269 6730 7865 8546 8737 826 Size: 284 461 1135 681 191 1471 Fragments arranged by size: 1471 1296 1254 1135 681 474 461 307 300 284 267 219 216 195 191 165 114 111 102 60 40 39 SfoI GGC'GCC Cuts at: 3881 5756 3881 Size: 1875 7507 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9382 SmaI CCC'GGG Cuts at: 1872 3170 5090 6188 6709 1872 Size: 1298 1920 1098 521 4545 Fragments arranged by size: 4545 1920 1298 1098 521 SmlI C'TyrA_G Cuts at: 820 1017 1943 2994 5006 5066 5270 5749 6227 Size: 197 926 1051 2012 60 204 479 478 Cuts at: 6227 7486 8387 8649 820 Size: 1259 901 262 1553 Fragments arranged by size: 2012 1553 1259 1051 926 901 479 478 262 204 197 60 SnaBI TAC'GTA Cuts at: 493 7173 493 Size: 6680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9382 SphI G_CATG'C Cuts at: 1132 6740 1132 Size: 5608 3774 SrfI GCCC'GGGC Cuts at: 5090 5090 Size: 9382 SspI AAT'ATT Cuts at: 5 52 2449 7306 5 Size: 47 2397 4857 2081 Fragments arranged by size: 4857 2397 2081 47 StuI AGG'CCT Cuts at: 1517 4646 5255 1517 Size: 3129 609 5644 Fragments arranged by size: 5644 3129 609 StyI C'CwwG_G Cuts at: 513 1065 2004 3087 3736 3820 4257 4839 5629 Size: 552 939 1083 649 84 437 582 790 Cuts at: 5629 6195 6821 513 Size: 566 626 3074 Fragments arranged by size: 3074 1083 939 790 649 626 582 566 552 437 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6544 6572 7409 7426 7579 7764 8209 Size: 1255 4964 28 837 17 153 185 445 Cuts at: 8209 325 Size: 1498 Fragments arranged by size: 4964 1498 1255 837 445 185 153 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3457 5376 7628 Size: 274 80 33 51 1443 1480 1919 2252 Cuts at: 7628 9340 96 Size: 1712 138 Fragments arranged by size: 2252 1919 1712 1480 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 6054 6086 6616 6648 9342 9374 2387 Size: 32 3635 32 530 32 2694 32 2395 Fragments arranged by size: 3635 2694 2395 530 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9382 XbaI T'CTAG_A Cuts at: 6718 6718 Size: 9382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2154 3259 4529 5700 5884 2154 Size: 1105 1270 1171 184 5652 Fragments arranged by size: 5652 1270 1171 1105 184 XhoI C'TCGA_G Cuts at: 1943 2994 6227 1943 Size: 1051 3233 5098 Fragments arranged by size: 5098 3233 1051 XmaI C'CCGG_G Cuts at: 1870 3168 5088 6186 6707 1870 Size: 1298 1920 1098 521 4545 Fragments arranged by size: 4545 1920 1298 1098 521 XmnI GAAnn'nnTTC Cuts at: 3272 7511 3272 Size: 4239 5143 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7190 276 Size: 53 83 186 936 5656 2468 Fragments arranged by size: 5656 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI FalI FseI FspAI NsiI PacI PmlI RsrII SfiI SwaI