Restriction map for
pFN21AB4616
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 18 CGCG CG^CG
AccIII 537 TCCGGA T^CCGGA
AfaI 560 GTAC GT^AC
AflIII 49 ACGTGT A^C[GA][CT]GT
AspHI 732 GAGCTC G[AT]GC[AT]^C
AvaI 752 CTCGGG C^[CT]CG[GA]G
BanI 1009 GGTGCC G^G[CT][GA]CC
BbrPI 48 CACGTG CAC^GTG
BbvI 582 GCAGC GCAGC(8/12)
BcnI 79 CCGGG CC^[GC]GG
BlpI 970 GCTGAGC GC^T[ACGT]AGC
BpmI 8 CTGGAG CTGGAG(16/14)
Bpu1102I 970 GCTGAGC GC^T[ACGT]AGC
BsaAI 48 CACGTG [CT]AC^GT[GA]
BsaWI 537 TCCGGA [AT]^CCGG[AT]
BseAI 537 TCCGGA T^CCGGA
BsiHKAI 732 GAGCTC G[AT]GC[AT]^C
BsmAI 334 GAGAC GTCTC(1/5)
BsmFI 755 GGGAC GGGAC(10/14)
BsmI 103 GAATGC GAATGC(1/-1)
BsoBI 752 CTCGGG C^[CT]CG[GA]G
BspEI 537 TCCGGA T^CCGGA
BsrDI 856 GCAATG GCAATG(2/0)
BstUI 18 CGCG CG^CG
CelII 970 GCTGAGC GC^T[ACGT]AGC
DpnII 702 GATC ^GATC
MboI 702 GATC ^GATC
MroI 537 TCCGGA T^CCGGA
MvnI 18 CGCG CG^CG
NciI 79 CCGGG CC^[GC]GG
NcoI 543 CCATGG C^CATGG
NdeII 702 GATC ^GATC
PflMI 604 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 48 CACGTG CAC^GTG
PmlI 48 CACGTG CAC^GTG
PpuMI 999 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 560 GTAC GT^AC
SacI 732 GAGCTC GAGCT^C
Sau3AI 702 GATC ^GATC
StuI 194 AGGCCT AGG^CCT
TseI 582 GCAGC G^C[AT]GC
Van91I 604 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 484 GAATTT [GA]^AATT[CT]
784 GAATTT
ApoI 484 GAATTT [GA]^AATT[CT]
784 GAATTT
BalI 521 TGGCCA TGG^CCA
860 TGGCCA
BbsI 379 GAAGAC GAAGAC(2/6)
427 GAAGAC
BfaI 643 CTAG C^TAG
838 CTAG
BpuAI 379 GAAGAC GAAGAC(2/6)
427 GAAGAC
BseRI 367 GAGGAG GAGGAG(10/8)
817 GAGGAG
Cac8I 62 GCGAGC GC[ACGT]^[ACGT]GC
207 GCCAGC
CfoI 17 GCGC GCG^C
139 GCGC
DraII 827 AGGCCCT [GA]G^G[ACGT]CC[CT]
999 AGGACCC
DsaI 183 CCACGG C^C[GA][CT]GG
543 CCATGG
EcoO109I 827 AGGCCCT [GA]G^G[ACGT]CC[CT]
999 AGGACCC
EcoT14I 543 CCATGG C^C[AT][AT]GG
938 CCAAGG
FauI 204 CCCGC CCCGC(4/6)
226 GCGGG
HapII 79 CCGG C^CGG
538 CCGG
HhaI 17 GCGC GCG^C
139 GCGC
HinP1I 17 GCGC G^CGC
139 GCGC
HpaII 79 CCGG C^CGG
538 CCGG
HphI 162 TCACC GGTGA(8/7)
618 TCACC
MaeI 643 CTAG C^TAG
838 CTAG
MaeII 49 ACGT A^CGT
179 ACGT
MluNI 521 TGGCCA TGG^CCA
860 TGGCCA
MscI 521 TGGCCA TGG^CCA
860 TGGCCA
MseI 664 TTAA T^TAA
788 TTAA
MspA1I 209 CAGCTG C[AC]G^C[GT]G
968 CAGCTG
MspI 79 CCGG C^CGG
538 CCGG
PvuII 209 CAGCTG CAG^CTG
968 CAGCTG
StyI 543 CCATGG C^C[AT][AT]GG
938 CCAAGG
TaqI 26 TCGA T^CGA
704 TCGA
TfiI 448 GAATC G^A[AT]TC
964 GAATC
Tru9I 664 TTAA T^TAA
788 TTAA
TspRI 1005 CAGTG CA[GC]TG[ACGT][ACGT]^
1014 CAGTG
TthHB8I 26 TCGA T^CGA
704 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AvaII BanII BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeIII
MboII MnlI MvaI MwoI NlaIII
NlaIV PleI Sau96I ScrFI Tsp45I
Tsp509I