(Circular) (Six-base) MAPSORT of: pFN21AB4616.seq Check: 8258 from: 1 to: 5737 pFN21AB4616 5737 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2714 3800 5184 5452 1632 Size: 1082 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1086 1082 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 2054 4636 5613 1203 Size: 851 2582 977 1327 Fragments arranged by size: 2582 1327 977 851 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 4466 4466 Size: 5737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AlwNI CAG_nnn'CTG Cuts at: 3480 5052 3480 Size: 1572 4165 ApaLI G'TGCA_C Cuts at: 3794 4950 3794 Size: 1156 4581 ApoI r'AATT_y Cuts at: 1493 2489 2789 3046 3272 3424 5533 1493 Size: 996 300 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 996 300 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2757 3062 1870 Size: 73 814 305 4545 Fragments arranged by size: 4545 814 305 73 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3014 3058 4513 618 Size: 325 217 627 1227 44 1455 1842 Fragments arranged by size: 1842 1455 1227 627 325 217 44 BanII G_rGCy'C Cuts at: 1391 2073 2623 2741 3056 1391 Size: 682 550 118 315 4072 Fragments arranged by size: 4072 682 550 315 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2391 2439 928 Size: 971 492 48 4226 Fragments arranged by size: 4226 971 492 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3928 3962 1903 Size: 34 1991 34 3678 Fragments arranged by size: 3678 1991 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4348 136 Size: 107 122 71 631 605 2676 1525 Fragments arranged by size: 2676 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5732 5732 Size: 5737 BlpI GC'TnA_GC Cuts at: 2976 3154 2976 Size: 178 5559 Bme1580I G_kGCm'C Cuts at: 1790 3798 4954 1790 Size: 2008 1156 2573 Fragments arranged by size: 2573 2008 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3003 3468 4426 Size: 416 53 83 399 24 1586 465 958 Cuts at: 4426 442 Size: 1753 Fragments arranged by size: 1753 1586 958 465 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 4397 1239 Size: 795 2363 2579 Fragments arranged by size: 2579 2363 795 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3862 4727 5025 3862 Size: 865 298 4574 Fragments arranged by size: 4574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4400 882 Size: 617 2901 2219 Fragments arranged by size: 2901 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2055 3528 493 Size: 711 851 1473 2702 Fragments arranged by size: 2702 1473 851 711 BsaBI GATnn'nnATC Cuts at: 5731 5731 Size: 5737 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3544 3926 4561 275 Size: 53 83 186 936 2011 382 635 1451 Fragments arranged by size: 2011 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2542 4170 4568 4842 4989 5400 2542 Size: 1628 398 274 147 411 2879 Fragments arranged by size: 2879 1628 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 1178 Size: 30 165 30 1221 30 4261 Fragments arranged by size: 4261 1221 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2387 2837 1186 Size: 1201 450 4086 Fragments arranged by size: 4086 1201 450 BseYI C'CCAG_C Cuts at: 4940 5436 4940 Size: 496 5241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3948 4976 1540 Size: 275 186 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2741 3798 3883 4954 2741 Size: 1057 85 1071 3524 Fragments arranged by size: 3524 1071 1057 85 BsmI GAATG_Cn' Cuts at: 2114 3248 3341 2114 Size: 1134 93 4510 Fragments arranged by size: 4510 1134 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2623 2741 3056 3798 3883 4954 Size: 399 283 550 118 315 742 85 1071 Cuts at: 4954 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 550 399 315 283 118 85 BspEI T'CCGG_A Cuts at: 2542 5400 2542 Size: 2858 2879 BspHI T'CATG_A Cuts at: 3626 5356 3626 Size: 1730 4007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3092 844 Size: 2248 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3624 1394 Size: 429 1801 3507 Fragments arranged by size: 3507 1801 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2868 4232 4406 65 Size: 1183 1620 1364 174 1396 Fragments arranged by size: 1620 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4381 4568 1700 Size: 110 2571 187 2869 Fragments arranged by size: 2869 2571 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5737 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstYI r'GATC_y Cuts at: 1972 3067 3434 3819 3836 5288 5374 5386 5732 Size: 1095 367 385 17 1452 86 12 346 Cuts at: 5732 1972 Size: 1977 Fragments arranged by size: 1977 1452 1095 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 2548 513 Size: 552 939 184 360 3702 Fragments arranged by size: 3702 939 552 360 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2382 3009 487 Size: 683 56 1156 627 3215 Fragments arranged by size: 3215 1156 683 627 56 BtsI GCAGTG_nn' Cuts at: 3253 4047 4067 3253 Size: 794 20 4923 Fragments arranged by size: 4923 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2526 2865 Size: 54 1425 50 275 18 345 351 339 Cuts at: 2865 3184 4073 5702 8 Size: 319 889 1629 43 Fragments arranged by size: 1629 1425 889 351 345 339 319 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 1537 Size: 275 1372 4090 Fragments arranged by size: 4090 1372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 2307 2325 2343 3021 3667 1353 Size: 936 18 18 18 678 646 3423 Fragments arranged by size: 3423 936 678 646 18 18 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2477 4324 4698 4844 387 Size: 1064 138 888 1847 374 146 1280 Fragments arranged by size: 1847 1280 1064 888 374 146 138 EcoICRI GAG'CTC Cuts at: 2739 2739 Size: 5737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2714 3800 4397 5184 5452 1239 Size: 393 402 680 1086 597 787 268 1524 Fragments arranged by size: 1524 1086 787 680 597 402 393 268 EcoO109I rG'GnC_Cy Cuts at: 2833 3005 2833 Size: 172 5565 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 4884 1678 Size: 3206 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2306 2320 2338 2524 Size: 32 135 32 716 18 14 18 186 Cuts at: 2524 2556 2888 2920 4378 4410 4452 4484 1373 Size: 32 332 32 1458 32 42 32 2626 Fragments arranged by size: 2626 1458 716 332 186 135 42 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5737 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2286 4851 5035 1305 Size: 981 2565 184 2007 Fragments arranged by size: 2565 2007 981 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 2867 10 Size: 54 1425 343 696 339 2880 Fragments arranged by size: 2880 1425 696 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 3695 4054 4213 518 Size: 648 2529 359 159 2042 Fragments arranged by size: 2529 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2975 3153 3830 4978 5223 1606 Size: 610 759 178 677 1148 245 2120 Fragments arranged by size: 2120 1148 759 677 610 245 178 NcoI C'CATG_G Cuts at: 513 1065 2004 2548 513 Size: 552 939 544 3702 Fragments arranged by size: 3702 939 552 544 NdeI CA'TA_TG Cuts at: 387 387 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 3095 4640 1132 Size: 97 1866 1545 2229 Fragments arranged by size: 2229 1866 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5737 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 2615 1108 Size: 1507 4230 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PmlI CAC'GTG Cuts at: 2055 2055 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3005 3005 Size: 5737 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PstI C_TGCA'G Cuts at: 830 3089 830 Size: 2259 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 PvuII CAG'CTG Cuts at: 2216 2975 2216 Size: 759 4978 SacI G_AGCT'C Cuts at: 2741 2741 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 3085 4220 4901 5092 826 Size: 219 2040 1135 681 191 1471 Fragments arranged by size: 2040 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 3064 1872 Size: 1192 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3841 4742 5004 820 Size: 197 926 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 3095 1132 Size: 1963 3774 SspI AAT'ATT Cuts at: 5 52 3661 5 Size: 47 3609 2081 Fragments arranged by size: 3609 2081 47 StuI AGG'CCT Cuts at: 1517 2201 1517 Size: 684 5053 StyI C'CwwG_G Cuts at: 513 1065 2004 2548 2943 3176 513 Size: 552 939 544 395 233 3074 Fragments arranged by size: 3074 939 552 544 395 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3764 3781 3934 4119 4564 325 Size: 1255 2184 17 153 185 445 1498 Fragments arranged by size: 2184 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3983 5695 96 Size: 274 80 33 51 1443 2006 1712 138 Fragments arranged by size: 2006 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2624 2656 5697 5729 2624 Size: 32 3041 32 2632 Fragments arranged by size: 3041 2632 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 3073 3073 Size: 5737 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 3062 1870 Size: 1192 4545 XmnI GAAnn'nnTTC Cuts at: 3866 3866 Size: 5737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI