Restriction map for
pFN21AE3692
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# NO CUT ENZYMES ( 172 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BbeI BbsI BbvCI BcgI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HaeII HgaI HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 392 GACGTC GACGT^C
AccII 5 CGCG CG^CG
AcyI 392 GACGTC G[GA]^CG[CT]C
AvaII 478 GGACC G^G[AT]CC
BamHI 383 GGATCC G^GATCC
BanI 91 GGCACC G^G[CT][GA]CC
BbrPI 463 CACGTG CAC^GTG
BciVI 399 GGATAC GTATCC(6/5)
BfaI 457 CTAG C^TAG
BglI 68 GCCAAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 463 CACGTG [CT]AC^GT[GA]
BsaHI 392 GACGTC G[GA]^CG[CT]C
BsaI 119 GGTCTC GGTCTC(1/5)
BseRI 10 CTCCTC GAGGAG(10/8)
BsmBI 390 GAGACG CGTCTC(1/5)
BsmFI 102 GGGAC GGGAC(10/14)
BsrDI 265 CATTGC GCAATG(2/0)
BstNI 481 CCTGG CC^[AT]GG
BstUI 5 CGCG CG^CG
DraII 26 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 321 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 289 CTCTTC CTCTTC(1/4)
EcoO109I 26 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 481 CCTGG ^CC[AT]GG
EcoT14I 535 CCAAGG C^C[AT][AT]GG
Hin1I 392 GACGTC G[GA]^CG[CT]C
Ksp632I 289 CTCTTC CTCTTC(1/4)
MaeI 457 CTAG C^TAG
MboII 290 TCTTC GAAGA(8/7)
MvaI 481 CCTGG CC^[AT]GG
MvnI 5 CGCG CG^CG
NlaIII 349 CATG CATG^
PmaCI 463 CACGTG CAC^GTG
PmlI 463 CACGTG CAC^GTG
ScaI 308 AGTACT AGT^ACT
SfaNI 506 GCATC GCATC(5/9)
StyI 535 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 309 GTAC GT^AC
353 GTAC
AflIII 130 ACGTGT A^C[GA][CT]GT
464 ACGTGT
ApaI 85 GGGCCC GGGCC^C
484 GGGCCC
BanII 85 GGGCCC G[GA]GC[CT]^C
484 GGGCCC
BbvI 138 GCTGC GCAGC(8/12)
275 GCTGC
BmyI 85 GGGCCC G[AGT]GC[ACT]^C
484 GGGCCC
BsmAI 390 GAGAC GTCTC(1/5)
120 GTCTC
Bsp1286I 85 GGGCCC G[AGT]GC[ACT]^C
484 GGGCCC
BsrI 105 ACTGG ACTGG(1/-1)
322 ACTGG
BstYI 230 GGATCT [GA]^GATC[CT]
383 GGATCC
CfoI 4 GCGC GCG^C
46 GCGC
DdeI 299 CTGAG C^T[ACGT]AG
388 CTGAG
HhaI 4 GCGC GCG^C
46 GCGC
HinP1I 4 GCGC G^CGC
46 GCGC
MaeIII 193 GTCAC ^GT[ACGT]AC
461 GTCAC
MflI 230 GGATCT [GA]^GATC[CT]
383 GGATCC
RsaI 309 GTAC GT^AC
353 GTAC
TseI 138 GCTGC G^C[AT]GC
275 GCTGC
Tsp45I 193 GTCAC ^GT[GC]AC
461 GTCAC
XhoII 230 GGATCT [GA]^GATC[CT]
383 GGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AlwI BcnI BsaJI
BsiYI BslI BssKI Cac8I Cfr13I
DpnII FauI Fnu4HI HaeIII HapII
HpaII ItaI MaeII MboI MnlI
MspI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI TspRI