(Circular) (Six-base) MAPSORT of: pFN21AB4518.seq Check: 4794 from: 1 to: 5719 pFN21AB4518 5719 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2333 2333 Size: 5719 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3062 1937 Size: 1125 4594 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 5166 5434 1632 Size: 2150 1384 268 1917 Fragments arranged by size: 2150 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 2227 4448 2227 Size: 2221 3498 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 2741 3028 3254 3406 5515 1493 Size: 1248 287 226 152 2109 1697 Fragments arranged by size: 2109 1697 1248 287 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2175 2361 3044 1870 Size: 73 232 186 683 4545 Fragments arranged by size: 4545 683 232 186 73 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3040 4495 618 Size: 325 217 627 1253 1455 1842 Fragments arranged by size: 1842 1455 1253 627 325 217 BanII G_rGCy'C Cuts at: 1391 3038 1391 Size: 1647 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 928 Size: 971 124 4624 Fragments arranged by size: 4624 971 124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2591 2625 3910 3944 1903 Size: 34 654 34 1285 34 3678 Fragments arranged by size: 3678 1285 654 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 2247 1548 Size: 699 5020 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2547 5714 2547 Size: 3167 2552 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2073 3780 4936 1790 Size: 283 1707 1156 2573 Fragments arranged by size: 2573 1707 1156 283 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2032 2266 3450 Size: 416 53 83 399 24 615 234 1184 Cuts at: 3450 4408 442 Size: 958 1753 Fragments arranged by size: 1753 1184 958 615 416 399 234 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2564 2941 2946 4379 1239 Size: 1325 377 5 1433 2579 Fragments arranged by size: 2579 1433 1325 377 5 Bpu10I CC'TnA_GC Cuts at: 2708 2708 Size: 5719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4709 5007 3844 Size: 865 298 4556 Fragments arranged by size: 4556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2559 4382 882 Size: 617 1060 1823 2219 Fragments arranged by size: 2219 1823 1060 617 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3526 3908 4543 275 Size: 53 83 186 936 1993 382 635 1451 Fragments arranged by size: 1993 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2058 2088 2939 2969 1178 Size: 30 165 30 655 30 851 30 3928 Fragments arranged by size: 3928 851 655 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 2398 1186 Size: 1193 19 4507 Fragments arranged by size: 4507 1193 19 BseYI C'CCAG_C Cuts at: 2213 4922 5418 2213 Size: 2709 496 2514 Fragments arranged by size: 2709 2514 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 1163 Size: 573 618 4528 Fragments arranged by size: 4528 618 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2965 3780 3865 4936 2965 Size: 815 85 1071 3748 Fragments arranged by size: 3748 1071 815 85 BsmI GAATG_Cn' Cuts at: 2591 3230 3323 2591 Size: 639 93 4987 Fragments arranged by size: 4987 639 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2965 3038 3780 3865 4936 1391 Size: 399 283 892 73 742 85 1071 2174 Fragments arranged by size: 2174 1071 892 742 399 283 85 73 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 2283 2397 3608 5338 2283 Size: 114 1211 1730 2664 Fragments arranged by size: 2664 1730 1211 114 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2333 3074 844 Size: 1489 741 3489 Fragments arranged by size: 3489 1489 741 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 2241 3779 4791 2241 Size: 1538 1012 3169 Fragments arranged by size: 3169 1538 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5719 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 2170 1184 Size: 986 4733 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2782 2965 2107 Size: 675 183 4861 Fragments arranged by size: 4861 675 183 BstYI r'GATC_y Cuts at: 1972 2547 3049 3416 3801 3818 5270 5356 5368 Size: 575 502 367 385 17 1452 86 12 Cuts at: 5368 5714 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 575 502 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2147 2147 Size: 5719 BtgI C'CryG_G Cuts at: 513 1065 2733 513 Size: 552 1668 3499 Fragments arranged by size: 3499 1668 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 2443 3235 4029 4049 2443 Size: 792 794 20 4113 Fragments arranged by size: 4113 794 792 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2874 3166 4055 Size: 54 1425 50 275 18 1044 292 889 Cuts at: 4055 5684 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1044 889 292 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 3649 1353 Size: 707 1589 3423 Fragments arranged by size: 3423 1589 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2941 2946 3782 4379 5166 5434 Size: 393 932 377 5 836 597 787 268 Cuts at: 5434 1239 Size: 1524 Fragments arranged by size: 1524 932 836 787 597 393 377 268 5 EcoO109I rG'GnC_Cy Cuts at: 2988 2988 Size: 5719 EcoRI G'AATT_C Cuts at: 2741 3028 3406 2741 Size: 287 378 5054 Fragments arranged by size: 5054 378 287 FspI TGC'GCA Cuts at: 2078 4225 2078 Size: 2147 3572 HaeII r_GCGC'y Cuts at: 1678 2142 4866 1678 Size: 464 2724 2531 Fragments arranged by size: 2724 2531 464 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2329 2361 4360 Size: 32 135 32 641 32 84 32 1999 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1999 641 135 84 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3063 3309 669 Size: 988 281 1125 246 3079 Fragments arranged by size: 3079 1125 988 281 246 HindIII A'AGCT_T Cuts at: 748 2885 748 Size: 2137 3582 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4833 5017 1305 Size: 3528 184 2007 Fragments arranged by size: 3528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2876 10 Size: 54 1425 343 1044 2853 Fragments arranged by size: 2853 1425 1044 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2420 3677 4036 4195 518 Size: 648 1254 1257 359 159 2042 Fragments arranged by size: 2042 1257 1254 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 3135 3812 4960 5205 1606 Size: 476 1053 677 1148 245 2120 Fragments arranged by size: 2120 1148 1053 677 476 245 NcoI C'CATG_G Cuts at: 513 1065 2733 513 Size: 552 1668 3499 Fragments arranged by size: 3499 1668 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2818 3077 4622 1132 Size: 97 1589 259 1545 2229 Fragments arranged by size: 2229 1589 1545 259 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 2296 1108 Size: 1188 4531 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2988 2988 Size: 5719 PshAI GACnn'nnGTC Cuts at: 1613 2313 2497 1613 Size: 700 184 4835 Fragments arranged by size: 4835 700 184 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PstI C_TGCA'G Cuts at: 830 2728 3071 830 Size: 1898 343 3478 Fragments arranged by size: 3478 1898 343 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 RsrII CG'GwC_CG Cuts at: 2122 2122 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SanDI GG'GwC_CC Cuts at: 2988 2988 Size: 5719 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 5719 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SexAI A'CCwGG_T Cuts at: 2765 2765 Size: 5719 SfcI C'TryA_G Cuts at: 826 1045 2724 3067 4202 4883 5074 826 Size: 219 1679 343 1135 681 191 1471 Fragments arranged by size: 1679 1471 1135 681 343 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 2177 2363 3046 1872 Size: 305 186 683 4545 Fragments arranged by size: 4545 683 305 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 3823 4724 4986 820 Size: 197 926 1880 901 262 1553 Fragments arranged by size: 1880 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2733 3158 513 Size: 552 1668 425 3074 Fragments arranged by size: 3074 1668 552 425 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 3746 3763 3916 4101 4546 325 Size: 1255 1101 1065 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1101 1065 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 3965 5677 Size: 274 80 33 51 1443 297 1691 1712 Cuts at: 5677 96 Size: 138 Fragments arranged by size: 1712 1691 1443 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2058 2090 2424 2456 5679 5711 2058 Size: 32 334 32 3223 32 2066 Fragments arranged by size: 3223 2066 334 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2484 2484 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 2175 2361 3044 1870 Size: 305 186 683 4545 Fragments arranged by size: 4545 683 305 186 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII SacI SacII SfiI SfoI SrfI SwaI