Restriction map for
pFN21AB4508
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BlnI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AgeI 115 ACCGGT A^CCGGT
AlwI 787 GGATC GGATC(4/5)
AseI 345 ATTAAT AT^TAAT
AsnI 345 ATTAAT AT^TAAT
AspHI 163 GTGCTC G[AT]GC[AT]^C
AspI 812 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 513 GGACC G^G[AT]CC
BalI 1031 TGGCCA TGG^CCA
BbsI 523 GTCTTC GAAGAC(2/6)
BbvCI 991 GCTGAGG CCTCAGC(-5/-2)
BcnI 189 CCGGG CC^[GC]GG
BglII 695 AGATCT A^GATCT
BpuAI 523 GTCTTC GAAGAC(2/6)
BsaI 303 GGTCTC GGTCTC(1/5)
BsaWI 115 ACCGGT [AT]^CCGG[AT]
BseRI 753 CTCCTC GAGGAG(10/8)
BsiHKAI 163 GTGCTC G[AT]GC[AT]^C
BspHI 1052 TCATGA T^CATGA
BspMI 1130 ACCTGC ACCTGC(4/8)
BsrFI 115 ACCGGT [GA]^CCGG[CT]
BstF5I 744 CATCC GGATG(2/0)
BstYI 695 AGATCT [GA]^GATC[CT]
CfoI 907 GCGC GCG^C
Cfr10I 115 ACCGGT [GA]^CCGG[CT]
DraII 512 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 514 GACCTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1031 TGGCCA [CT]^GGCC[GA]
EcoO109I 512 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 492 GAATTC G^AATTC
EcoT14I 715 CCATGG C^C[AT][AT]GG
FauI 808 GCGGG CCCGC(4/6)
FokI 744 CATCC GGATG(9/13)
HaeII 906 GGCGCT [GA]GCGC^[CT]
HhaI 907 GCGC GCG^C
HinP1I 907 GCGC G^CGC
HincII 214 GTCAAC GT[CT]^[GA]AC
HindII 214 GTCAAC GT[CT]^[GA]AC
MflI 695 AGATCT [GA]^GATC[CT]
MluNI 1031 TGGCCA TGG^CCA
MscI 1031 TGGCCA TGG^CCA
MspA1I 806 CAGCGG C[AC]G^C[GT]G
NciI 189 CCGGG CC^[GC]GG
NcoI 715 CCATGG C^CATGG
NspI 774 GCATGC [GA]CATG^[CT]
PflFI 812 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 924 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 115 ACCGGT A^CCGGT
PpuMI 512 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 345 ATTAAT AT^TAAT
RcaI 1052 TCATGA T^CATGA
ScaI 390 AGTACT AGT^ACT
SexAI 59 ACCAGGT A^CC[AT]GGT
SfcI 620 CTACAG C^T[GA][CT]AG
SgrAI 114 CACCGGTG C[GA]^CCGG[CT]G
SmlI 480 CTCAAG C^T[CT][GA]AG
SphI 774 GCATGC GCATG^C
StyI 715 CCATGG C^C[AT][AT]GG
Tth111I 812 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 924 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 695 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 1119 CCGC CCGC(-3/-1)
808 GCGG
AcsI 492 GAATTC [GA]^AATT[CT]
1065 AAATTC
ApoI 492 GAATTC [GA]^AATT[CT]
1065 AAATTC
BanII 275 GGGCTC G[GA]GC[CT]^C
358 GGGCTC
BbvI 805 GCAGC GCAGC(8/12)
778 GCTGC
BlpI 327 GCTCAGC GC^T[ACGT]AGC
501 GCTCAGC
BpmI 166 CTCCAG CTGGAG(16/14)
658 CTGGAG
Bpu1102I 327 GCTCAGC GC^T[ACGT]AGC
501 GCTCAGC
BsmAI 419 GAGAC GTCTC(1/5)
304 GTCTC
BsmFI 512 GGGAC GGGAC(10/14)
810 GGGAC
BstXI 308 CCACTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
377 CCACCCTGGTGG
CelII 327 GCTCAGC GC^T[ACGT]AGC
501 GCTCAGC
DpnII 696 GATC ^GATC
788 GATC
DsaI 158 CCGTGG C^C[GA][CT]GG
715 CCATGG
Fnu4HI 778 GCTGC GC^[ACGT]GC
805 GCAGC
HapII 116 CCGG C^CGG
189 CCGG
HpaII 116 CCGG C^CGG
189 CCGG
ItaI 778 GCTGC GC^[ACGT]GC
805 GCAGC
MboI 696 GATC ^GATC
788 GATC
MseI 146 TTAA T^TAA
346 TTAA
MspI 116 CCGG C^CGG
189 CCGG
MwoI 877 GCCTGCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
909 GCTCACCTGGC
NdeII 696 GATC ^GATC
788 GATC
NlaIV 512 GGGACC GG[ACGT]^[ACGT]CC
739 GGAGCC
Sau3AI 696 GATC ^GATC
788 GATC
Tru9I 146 TTAA T^TAA
346 TTAA
TseI 778 GCTGC G^C[AT]GC
805 GCAGC
Tsp45I 112 GTCAC ^GT[GC]AC
119 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BmyI BsaJI BsiYI
BslI Bsp1286I BsrDI BsrI BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
HaeIII HinfI HphI MaeIII MboII
MnlI MslI MvaI NlaIII PleI
RsaI Sau96I ScrFI SfaNI TfiI
Tsp509I TspRI XcmI