(Circular) (Six-base) MAPSORT of: pFN21AB4508.seq Check: 7003 from: 1 to: 5872 pFN21AB4508 5872 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3215 1937 Size: 1278 4594 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3135 3935 5319 5587 1632 Size: 1503 800 1384 268 1917 Fragments arranged by size: 1917 1503 1384 800 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 2120 4703 2120 Size: 2583 3289 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 2387 2770 1166 Size: 1221 383 4268 Fragments arranged by size: 4268 1221 383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 2247 Size: 32 5840 AlwNI CAG_nnn'CTG Cuts at: 3615 5187 3615 Size: 1572 4300 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 2497 3070 3181 3407 3559 5668 1493 Size: 1004 573 111 226 152 2109 1697 Fragments arranged by size: 2109 1697 1004 573 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 2351 4426 160 Size: 872 1319 2075 1606 Fragments arranged by size: 2075 1606 1319 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 3197 1870 Size: 73 1254 4545 Fragments arranged by size: 4545 1254 73 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3193 4648 618 Size: 325 217 627 1406 1455 1842 Fragments arranged by size: 1842 1455 1406 627 325 217 BanII G_rGCy'C Cuts at: 1391 2284 2367 3191 1391 Size: 893 83 824 4072 Fragments arranged by size: 4072 893 824 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2521 928 Size: 971 622 4279 Fragments arranged by size: 4279 971 622 BbvCI CC'TCA_GC Cuts at: 2997 2997 Size: 5872 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2700 5867 2700 Size: 3167 2705 BlpI GC'TnA_GC Cuts at: 2333 2507 3289 2333 Size: 174 782 4916 Fragments arranged by size: 4916 782 174 Bme1580I G_kGCm'C Cuts at: 1790 2662 2953 3933 5089 1790 Size: 872 291 980 1156 2573 Fragments arranged by size: 2573 1156 980 872 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3603 4561 442 Size: 416 53 83 399 24 2186 958 1753 Fragments arranged by size: 2186 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2684 4532 1239 Size: 917 528 1848 2579 Fragments arranged by size: 2579 1848 917 528 Bpu10I CC'TnA_GC Cuts at: 2997 2997 Size: 5872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2470 3997 4862 5160 2470 Size: 1527 865 298 3182 Fragments arranged by size: 3182 1527 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2314 4535 882 Size: 617 815 2221 2219 Fragments arranged by size: 2221 2219 815 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2120 4305 4703 4977 5124 5535 2120 Size: 2185 398 274 147 411 2457 Fragments arranged by size: 2457 2185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2614 2644 1178 Size: 30 165 30 1211 30 4406 Fragments arranged by size: 4406 1211 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2749 1186 Size: 1563 4309 BseYI C'CCAG_C Cuts at: 2296 2800 5075 5571 2296 Size: 504 2275 496 2597 Fragments arranged by size: 2597 2275 504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 3933 4018 5089 2172 Size: 1761 85 1071 2955 Fragments arranged by size: 2955 1761 1071 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2284 2367 2662 2953 3191 3933 Size: 399 382 112 83 295 291 238 742 Cuts at: 3933 4018 5089 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 295 291 238 112 85 83 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 3057 3761 5491 3057 Size: 704 1730 3438 Fragments arranged by size: 3438 1730 704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3144 3227 844 Size: 2300 83 3489 Fragments arranged by size: 3489 2300 83 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2795 2886 2898 4367 4541 65 Size: 1183 1547 91 12 1469 174 1396 Fragments arranged by size: 1547 1469 1396 1183 174 91 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 4516 4703 1700 Size: 110 310 2396 187 2869 Fragments arranged by size: 2869 2396 310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5872 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2389 2320 Size: 69 5803 BstYI r'GATC_y Cuts at: 1972 2700 3202 3569 3954 3971 5423 5509 5521 Size: 728 502 367 385 17 1452 86 12 Cuts at: 5521 5867 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 728 502 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2163 2720 513 Size: 552 939 159 557 3665 Fragments arranged by size: 3665 939 557 552 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5133 Fragments arranged by size: 5133 683 56 BtsI GCAGTG_nn' Cuts at: 3388 4182 4202 3388 Size: 794 20 5058 Fragments arranged by size: 5058 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 4879 809 Size: 507 1209 2354 1802 Fragments arranged by size: 2354 1802 1209 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3036 3319 4208 Size: 54 1425 50 275 18 1206 283 889 Cuts at: 4208 5837 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1206 889 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3802 1353 Size: 2449 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2684 3135 3935 4532 5319 5587 Size: 393 524 528 451 800 597 787 268 Cuts at: 5587 1239 Size: 1524 Fragments arranged by size: 1524 800 787 597 528 524 451 393 268 EcoO109I rG'GnC_Cy Cuts at: 2518 2518 Size: 5872 EcoRI G'AATT_C Cuts at: 2497 3181 3559 2497 Size: 684 378 4810 Fragments arranged by size: 4810 684 378 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2915 5019 1678 Size: 1237 2104 2531 Fragments arranged by size: 2531 2104 1237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373 Size: 32 135 32 2941 32 42 32 2626 Fragments arranged by size: 2941 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2221 3216 3462 669 Size: 988 281 283 995 246 3079 Fragments arranged by size: 3079 995 988 283 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2983 3099 4986 5170 1305 Size: 1678 116 1887 184 2007 Fragments arranged by size: 2007 1887 1678 184 116 MscI TGG'CCA Cuts at: 10 64 1489 1832 3038 10 Size: 54 1425 343 1206 2844 Fragments arranged by size: 2844 1425 1206 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2387 2770 2891 3830 4189 4348 Size: 648 1152 69 383 121 939 359 159 Cuts at: 4348 518 Size: 2042 Fragments arranged by size: 2042 1152 939 648 383 359 159 121 69 MspA1I CmG'CkG Cuts at: 1606 2813 3288 3965 5113 5358 1606 Size: 1207 475 677 1148 245 2120 Fragments arranged by size: 2120 1207 1148 677 475 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2720 513 Size: 552 939 716 3665 Fragments arranged by size: 3665 939 716 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 2783 3230 4775 1132 Size: 97 1554 447 1545 2229 Fragments arranged by size: 2229 1554 1545 447 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5872 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2935 1108 Size: 1827 4045 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 2518 Size: 5872 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PstI C_TGCA'G Cuts at: 830 3224 830 Size: 2394 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 2397 4120 5832 2397 Size: 1723 1712 2437 Fragments arranged by size: 2437 1723 1712 SexAI A'CCwGG_T Cuts at: 2064 2064 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 2625 3220 4355 5036 5227 826 Size: 219 1580 595 1135 681 191 1471 Fragments arranged by size: 1580 1471 1135 681 595 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 2120 1700 Size: 420 5452 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2485 3976 4877 5139 820 Size: 197 926 542 1491 901 262 1553 Fragments arranged by size: 1553 1491 926 901 542 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 2783 3230 1132 Size: 1651 447 3774 Fragments arranged by size: 3774 1651 447 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2004 2720 3311 513 Size: 552 939 716 591 3074 Fragments arranged by size: 3074 939 716 591 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3899 3916 4069 4254 4699 325 Size: 1255 2319 17 153 185 445 1498 Fragments arranged by size: 2319 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2395 4118 5830 Size: 274 80 33 51 1443 418 1723 1712 Cuts at: 5830 96 Size: 138 Fragments arranged by size: 1723 1712 1443 418 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 2820 1316 Size: 1504 4368 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2640 3018 3151 2640 Size: 378 133 5361 Fragments arranged by size: 5361 378 133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI