(Linear) (Six-base) MAPSORT of: KIBB4508.seq  Check: 1609  from: 1  to: 1167



KIBB4508.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1130    1167 
   Size:       1130      37
 
AgeI A'CCGG_T

Cuts at:      0     115    1167 
   Size:        115    1052
 
AleI CACnn'nnGTG

Cuts at:      0     382     765    1167 
   Size:        382     383     402

  Fragments arranged by size: 

                 402     383     382
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     242     274    1167 
   Size:        242      32     893

  Fragments arranged by size: 

                 893     242      32
 
ApoI r'AATT_y

Cuts at:      0     492    1065    1167 
   Size:        492     573     102

  Fragments arranged by size: 

                 573     492     102
 
AseI AT'TA_AT

Cuts at:      0     346    1167 
   Size:        346     821
 
BanII G_rGCy'C

Cuts at:      0     279     362    1167 
   Size:        279      83     805

  Fragments arranged by size: 

                 805     279      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     516    1167 
   Size:        516     651
 
BbvCI CC'TCA_GC

Cuts at:      0     992    1167 
   Size:        992     175
 
BglII A'GATC_T

Cuts at:      0     695    1167 
   Size:        695     472
 
BlpI GC'TnA_GC

Cuts at:      0     328     502    1167 
   Size:        328     174     665

  Fragments arranged by size: 

                 665     328     174
 
Bme1580I G_kGCm'C

Cuts at:      0     657     948    1167 
   Size:        657     291     219

  Fragments arranged by size: 

                 657     291     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     679    1167 
   Size:        151     528     488

  Fragments arranged by size: 

                 528     488     151
 
Bpu10I CC'TnA_GC

Cuts at:      0     992    1167 
   Size:        992     175
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465    1167 
   Size:        465     702
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     309    1167 
   Size:        309     858
 
BsaWI w'CCGG_w

Cuts at:      0     115    1167 
   Size:        115    1052
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     609     639    1167 
   Size:        609      30     528

  Fragments arranged by size: 

                 609     528      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     744    1167 
   Size:        744     423
 
BseYI C'CCAG_C

Cuts at:      0     291     795    1167 
   Size:        291     504     372

  Fragments arranged by size: 

                 504     372     291
 
BsiHKAI G_wGCw'C

Cuts at:      0     167    1167 
   Size:        167    1000
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     279     362     657     948    1167 
   Size:        167     112      83     295     291     219

  Fragments arranged by size: 

                 295     291     219     167     112      83
 
BspHI T'CATG_A

Cuts at:      0    1052    1167 
   Size:       1052     115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1139    1167 
   Size:       1139      28
 
BsrDI GCAATG_nn'

Cuts at:      0     790     881     893    1167 
   Size:        790      91      12     274

  Fragments arranged by size: 

                 790     274      91      12
 
BsrFI r'CCGG_y

Cuts at:      0     115    1167 
   Size:        115    1052
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     315     384    1167 
   Size:        315      69     783

  Fragments arranged by size: 

                 783     315      69
 
BstYI r'GATC_y

Cuts at:      0     695    1167 
   Size:        695     472
 
BtgI C'CryG_G

Cuts at:      0     158     715    1167 
   Size:        158     557     452

  Fragments arranged by size: 

                 557     452     158
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     520    1167 
   Size:        520     647
 
EaeI y'GGCC_r

Cuts at:      0    1031    1167 
   Size:       1031     136
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     679    1130    1167 
   Size:        151     528     451      37

  Fragments arranged by size: 

                 528     451     151      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0     513    1167 
   Size:        513     654
 
EcoRI G'AATT_C

Cuts at:      0     492    1167 
   Size:        492     675
 
HaeII r_GCGC'y

Cuts at:      0     910    1167 
   Size:        910     257
 
HincII GTy'rAC

Cuts at:      0     216    1167 
   Size:        216     951
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     978    1094    1167 
   Size:        978     116      73

  Fragments arranged by size: 

                 978     116      73
 
MscI TGG'CCA

Cuts at:      0    1033    1167 
   Size:       1033     134
 
MslI CAynn'nnrTG

Cuts at:      0     313     382     765     886    1167 
   Size:        313      69     383     121     281

  Fragments arranged by size: 

                 383     313     281     121      69
 
MspA1I CmG'CkG

Cuts at:      0     808    1167 
   Size:        808     359
 
NcoI C'CATG_G

Cuts at:      0     715    1167 
   Size:        715     452
 
NspI r_CATG'y

Cuts at:      0     778    1167 
   Size:        778     389
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     930    1167 
   Size:        930     237
 
PpuMI rG'GwC_Cy

Cuts at:      0     513    1167 
   Size:        513     654
 
ScaI AGT'ACT

Cuts at:      0     392    1167 
   Size:        392     775
 
SexAI A'CCwGG_T

Cuts at:      0      59    1167 
   Size:         59    1108
 
SfcI C'TryA_G

Cuts at:      0     620    1167 
   Size:        620     547
 
SgrAI Cr'CCGG_yG

Cuts at:      0     115    1167 
   Size:        115    1052
 
SmlI C'TyrA_G

Cuts at:      0     480    1167 
   Size:        480     687
 
SphI G_CATG'C

Cuts at:      0     778    1167 
   Size:        778     389
 
StyI C'CwwG_G

Cuts at:      0     715    1167 
   Size:        715     452
 
TatI w'GTAC_w

Cuts at:      0     390    1167 
   Size:        390     777
 
Tth111I GACn'n_nGTC

Cuts at:      0     815    1167 
   Size:        815     352
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     635    1013    1146    1167 
   Size:        635     378     133      21

  Fragments arranged by size: 

                 635     378     133      21
 

 Enzymes that do cut:

     AcuI     AgeI     AleI     AloI     ApoI     AseI    BanII     BbsI
    BbvCI    BglII     BlpI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI
    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI    BstXI    BstYI     BtgI     DrdI     EaeI  Eco57MI
 EcoO109I    EcoRI    HaeII   HincII     MmeI     MscI     MslI   MspA1I
     NcoI     NspI    PflMI    PpuMI     ScaI    SexAI     SfcI    SgrAI
     SmlI     SphI     StyI     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI
    BsaHI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI