(Circular) (Six-base) MAPSORT of: pFN21AB4501.seq Check: 500 from: 1 to: 5299 pFN21AB4501 5299 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3109 278 Size: 53 83 186 936 1573 2468 Fragments arranged by size: 2468 1573 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2642 1937 Size: 705 4594 Acc65I G'GTAC_C Cuts at: 2620 2620 Size: 5299 AclI AA'CG_TT Cuts at: 1563 3426 3799 1563 Size: 1863 373 3063 Fragments arranged by size: 3063 1863 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3362 4746 5014 1632 Size: 1730 1384 268 1917 Fragments arranged by size: 1917 1730 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5102 AflIII A'CryG_T Cuts at: 1203 4198 5175 1203 Size: 2995 977 1327 Fragments arranged by size: 2995 1327 977 AgeI A'CCGG_T Cuts at: 4130 4130 Size: 5299 AhdI GACnn_n'nnGTC Cuts at: 4028 4028 Size: 5299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5299 AlwNI CAG_nnn'CTG Cuts at: 3042 4614 3042 Size: 1572 3727 ApaLI G'TGCA_C Cuts at: 3356 4512 3356 Size: 1156 4143 ApoI r'AATT_y Cuts at: 1493 2309 2608 2834 2986 5095 1493 Size: 816 299 226 152 2109 1697 Fragments arranged by size: 2109 1697 816 299 226 152 AseI AT'TA_AT Cuts at: 160 1032 3853 160 Size: 872 2821 1606 Fragments arranged by size: 2821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 AvaI C'yCGr_G Cuts at: 1870 1943 2439 2624 1870 Size: 73 496 185 4545 Fragments arranged by size: 4545 496 185 73 BamHI G'GATC_C Cuts at: 2629 2996 2629 Size: 367 4932 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2620 4075 618 Size: 325 217 627 833 1455 1842 Fragments arranged by size: 1842 1455 833 627 325 217 BanII G_rGCy'C Cuts at: 1391 2618 1391 Size: 1227 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 2345 928 Size: 971 389 57 3882 Fragments arranged by size: 3882 971 389 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2094 2128 3490 3524 1903 Size: 34 157 34 1362 34 3678 Fragments arranged by size: 3678 1362 157 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3191 4401 3191 Size: 1210 4089 BclI T'GATC_A Cuts at: 1548 1548 Size: 5299 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3910 136 Size: 107 122 71 631 605 2238 1525 Fragments arranged by size: 2238 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2566 5294 2566 Size: 2728 2571 BlpI GC'TnA_GC Cuts at: 2716 2716 Size: 5299 Bme1580I G_kGCm'C Cuts at: 1790 3360 4516 1790 Size: 1570 1156 2573 Fragments arranged by size: 2573 1570 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5299 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 3030 3988 Size: 416 53 83 399 24 700 913 958 Cuts at: 3988 442 Size: 1753 Fragments arranged by size: 1753 958 913 700 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5299 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2545 2545 Size: 5299 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3959 1239 Size: 2720 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3424 4289 4587 2168 Size: 1256 865 298 2880 Fragments arranged by size: 2880 1256 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2368 3962 882 Size: 617 869 1594 2219 Fragments arranged by size: 2219 1594 869 617 BsaAI yAC'GTr Cuts at: 493 1204 3090 493 Size: 711 1886 2702 Fragments arranged by size: 2702 1886 711 BsaBI GATnn'nnATC Cuts at: 5293 5293 Size: 5299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3106 3488 4123 275 Size: 53 83 186 936 1573 382 635 1451 Fragments arranged by size: 1573 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3732 4130 4404 4551 4962 3732 Size: 398 274 147 411 4069 Fragments arranged by size: 4069 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2581 2611 1178 Size: 30 165 30 1178 30 3866 Fragments arranged by size: 3866 1178 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 2342 2402 2602 1186 Size: 1126 30 60 200 3883 Fragments arranged by size: 3883 1126 200 60 30 BseYI C'CCAG_C Cuts at: 4502 4998 4502 Size: 496 4803 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2423 1163 Size: 573 687 4039 Fragments arranged by size: 4039 687 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2749 3510 4538 1540 Size: 275 186 748 761 1028 2301 Fragments arranged by size: 2301 1028 761 748 275 186 BsiHKAI G_wGCw'C Cuts at: 3360 3445 4516 3360 Size: 85 1071 4143 Fragments arranged by size: 4143 1071 85 BsmI GAATG_Cn' Cuts at: 2264 2810 2903 2264 Size: 546 93 4660 Fragments arranged by size: 4660 546 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2618 3360 3445 4516 1391 Size: 399 828 742 85 1071 2174 Fragments arranged by size: 2174 1071 828 742 399 85 BspEI T'CCGG_A Cuts at: 4962 4962 Size: 5299 BspHI T'CATG_A Cuts at: 3188 4918 3188 Size: 1730 3569 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2469 2654 844 Size: 1625 185 3489 Fragments arranged by size: 3489 1625 185 BsrBI CCG'CTC Cuts at: 1394 1823 3186 1394 Size: 429 1363 3507 Fragments arranged by size: 3507 1363 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3794 3968 65 Size: 1183 2546 174 1396 Fragments arranged by size: 2546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3943 4130 1700 Size: 110 2133 187 2869 Fragments arranged by size: 2869 2133 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5299 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5299 BssSI C'ACGA_G Cuts at: 2134 2449 3359 4371 2134 Size: 315 910 1012 3062 Fragments arranged by size: 3062 1012 910 315 BstAPI GCAn_nnn'nTGC Cuts at: 794 2270 794 Size: 1476 3823 BstBI TT'CG_AA Cuts at: 4103 4103 Size: 5299 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5299 BstXI CCAn_nnnn'nTGG Cuts at: 2045 2045 Size: 5299 BstYI r'GATC_y Cuts at: 1972 2379 2566 2629 2996 3381 3398 4850 4936 Size: 407 187 63 367 385 17 1452 86 Cuts at: 4936 4948 5294 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 407 385 367 346 187 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2058 2546 2058 Size: 488 4811 BtgI C'CryG_G Cuts at: 513 1065 2411 513 Size: 552 1346 3401 Fragments arranged by size: 3401 1346 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4560 Fragments arranged by size: 4560 683 56 BtsI GCAGTG_nn' Cuts at: 2815 3609 3629 2815 Size: 794 20 4485 Fragments arranged by size: 4485 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5299 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5264 DraI TTT'AAA Cuts at: 2603 2603 Size: 5299 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4306 809 Size: 507 2990 1802 Fragments arranged by size: 2990 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2746 3635 Size: 54 1425 50 275 18 244 672 889 Cuts at: 3635 5264 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 672 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2746 1537 Size: 275 934 4090 Fragments arranged by size: 4090 934 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3229 1353 Size: 1876 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3886 4260 4406 387 Size: 1064 138 2297 374 146 1280 Fragments arranged by size: 2297 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3362 3959 4746 5014 1239 Size: 393 1730 597 787 268 1524 Fragments arranged by size: 1730 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2309 2608 2986 2309 Size: 299 378 4622 Fragments arranged by size: 4622 378 299 FspI TGC'GCA Cuts at: 3805 3805 Size: 5299 HaeII r_GCGC'y Cuts at: 1678 4446 1678 Size: 2768 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 2558 2590 3940 Size: 32 135 32 514 32 440 32 1350 Cuts at: 3940 3972 4014 4046 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 514 440 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2349 2643 2889 669 Size: 988 281 411 294 246 3079 Fragments arranged by size: 3079 988 411 294 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5299 HpaI GTT'AAC Cuts at: 2889 2889 Size: 5299 KpnI G_GTAC'C Cuts at: 2624 2624 Size: 5299 MfeI C'AATT_G Cuts at: 2898 2898 Size: 5299 MluI A'CGCG_T Cuts at: 5175 5175 Size: 5299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4413 4597 1305 Size: 3108 184 2007 Fragments arranged by size: 3108 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2076 10 Size: 54 1425 343 244 3233 Fragments arranged by size: 3233 1425 343 244 54 MslI CAynn'nnrTG Cuts at: 518 1166 2250 3257 3616 3775 518 Size: 648 1084 1007 359 159 2042 Fragments arranged by size: 2042 1084 1007 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2715 3392 4540 4785 1606 Size: 1109 677 1148 245 2120 Fragments arranged by size: 2120 1148 1109 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4747 NdeI CA'TA_TG Cuts at: 387 387 Size: 5299 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5299 NotI GC'GGCC_GC Cuts at: 2746 2746 Size: 5299 NspI r_CATG'y Cuts at: 1132 1229 2657 4202 1132 Size: 97 1428 1545 2229 Fragments arranged by size: 2229 1545 1428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5299 PciI A'CATG_T Cuts at: 4198 4198 Size: 5299 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5299 PmeI GTTT'AAAC Cuts at: 2603 2603 Size: 5299 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3400 4881 4913 3368 Size: 32 1481 32 3754 Fragments arranged by size: 3754 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5299 PsiI TTA'TAA Cuts at: 2869 2869 Size: 5299 PstI C_TGCA'G Cuts at: 830 2466 2651 830 Size: 1636 185 3478 Fragments arranged by size: 3478 1636 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5299 SalI G'TCGA_C Cuts at: 1936 2641 1936 Size: 705 4594 SbfI CC_TGCA'GG Cuts at: 2466 2651 2466 Size: 185 5114 ScaI AGT'ACT Cuts at: 3547 5259 3547 Size: 1712 3587 SexAI A'CCwGG_T Cuts at: 2342 2342 Size: 5299 SfcI C'TryA_G Cuts at: 826 1045 2462 2647 3782 4463 4654 826 Size: 219 1417 185 1135 681 191 1471 Fragments arranged by size: 1471 1417 1135 681 219 191 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5299 SmaI CCC'GGG Cuts at: 1872 2626 1872 Size: 754 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 3403 4304 4566 820 Size: 197 926 240 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 262 240 197 SnaBI TAC'GTA Cuts at: 493 3090 493 Size: 2597 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5299 SphI G_CATG'C Cuts at: 1132 2657 1132 Size: 1525 3774 SspI AAT'ATT Cuts at: 5 52 3223 5 Size: 47 3171 2081 Fragments arranged by size: 3171 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5299 StyI C'CwwG_G Cuts at: 513 1065 2497 2738 513 Size: 552 1432 241 3074 Fragments arranged by size: 3074 1432 552 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3326 3343 3496 3681 4126 325 Size: 1255 1746 17 153 185 445 1498 Fragments arranged by size: 1746 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2506 2559 3545 Size: 274 80 33 51 1443 529 53 986 Cuts at: 3545 5257 96 Size: 1712 138 Fragments arranged by size: 1712 1443 986 529 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2581 2613 5259 5291 2581 Size: 32 2646 32 2589 Fragments arranged by size: 2646 2589 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2344 1316 Size: 1028 4271 XbaI T'CTAG_A Cuts at: 2635 2635 Size: 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5299 XmaI C'CCGG_G Cuts at: 1870 2624 1870 Size: 754 4545 XmnI GAAnn'nnTTC Cuts at: 3428 3428 Size: 5299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3107 276 Size: 53 83 186 936 1573 2468 Fragments arranged by size: 2468 1573 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI