(Circular) (Six-base) MAPSORT of: pFN21AB4480.seq Check: 4791 from: 1 to: 5782 pFN21AB4480 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 3125 1937 Size: 921 267 4594 Fragments arranged by size: 4594 921 267 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 3845 5229 5497 1632 Size: 780 1433 1384 268 1917 Fragments arranged by size: 1917 1433 1384 780 268 AflII C'TTAA_G Cuts at: 820 1017 2738 820 Size: 197 1721 3864 Fragments arranged by size: 3864 1721 197 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2752 2784 2752 Size: 32 5750 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2443 2633 2886 3011 3091 3317 3469 5578 Size: 950 190 253 125 80 226 152 2109 Cuts at: 5578 1493 Size: 1697 Fragments arranged by size: 2109 1697 950 253 226 190 152 125 80 AseI AT'TA_AT Cuts at: 160 1032 2732 4336 160 Size: 872 1700 1604 1606 Fragments arranged by size: 1700 1606 1604 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2346 3103 4558 618 Size: 325 217 627 559 757 1455 1842 Fragments arranged by size: 1842 1455 757 627 559 325 217 BanII G_rGCy'C Cuts at: 1391 3049 3101 1391 Size: 1658 52 4072 Fragments arranged by size: 4072 1658 52 BbeI G_GCGC'C Cuts at: 2350 2350 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2840 928 Size: 971 941 3870 Fragments arranged by size: 3870 971 941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3973 4007 1903 Size: 34 50 34 1952 34 3678 Fragments arranged by size: 3678 1952 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BfrBI ATG'CAT Cuts at: 2729 2729 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 3843 4999 1790 Size: 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4442 1239 Size: 3203 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2713 3573 493 Size: 711 1509 860 2702 Fragments arranged by size: 2702 1509 860 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2347 3589 3971 4606 Size: 53 83 186 936 814 1242 382 635 Cuts at: 4606 275 Size: 1451 Fragments arranged by size: 1451 1242 936 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2367 2397 1178 Size: 30 165 30 964 30 4563 Fragments arranged by size: 4563 964 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2715 3057 1186 Size: 919 610 342 3911 Fragments arranged by size: 3911 919 610 342 BseYI C'CCAG_C Cuts at: 2837 4985 5481 2837 Size: 2148 496 3138 Fragments arranged by size: 3138 2148 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2020 2451 1163 Size: 573 284 431 4494 Fragments arranged by size: 4494 573 431 284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 3049 3843 3928 4999 2097 Size: 952 794 85 1071 2880 Fragments arranged by size: 2880 1071 952 794 85 BsmI GAATG_Cn' Cuts at: 2451 3006 3293 3386 2451 Size: 555 287 93 4847 Fragments arranged by size: 4847 555 287 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2096 2096 Size: 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 3049 3101 3843 3928 4999 1391 Size: 399 307 952 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 952 742 399 307 85 52 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 2368 3671 5401 2368 Size: 1303 1730 2749 Fragments arranged by size: 2749 1730 1303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 3137 844 Size: 1562 731 3489 Fragments arranged by size: 3489 1562 731 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 2587 96 Size: 2491 3291 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 2173 2611 4586 2173 Size: 438 1975 3369 Fragments arranged by size: 3369 1975 438 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2859 2859 Size: 5782 Bsu36I CC'TnA_GG Cuts at: 2916 2916 Size: 5782 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 2925 3086 2925 Size: 161 5621 DraIII CAC_nnn'GTG Cuts at: 2335 2335 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747 Size: 54 1425 50 275 18 1399 889 1629 Cuts at: 5747 8 Size: 43 Fragments arranged by size: 1629 1425 1399 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2191 3712 1353 Size: 662 176 1521 3423 Fragments arranged by size: 3423 1521 662 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3047 3047 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 3845 4442 5229 5497 1239 Size: 393 780 1433 597 787 268 1524 Fragments arranged by size: 1524 1433 787 780 597 393 268 EcoRI G'AATT_C Cuts at: 3011 3091 3469 3011 Size: 80 378 5324 Fragments arranged by size: 5324 378 80 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2350 4929 1678 Size: 672 2579 2531 Fragments arranged by size: 2579 2531 672 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2473 2505 4423 4455 4497 Size: 32 135 32 901 32 1918 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1918 901 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 2346 2346 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2195 2419 4896 5080 1305 Size: 890 224 2477 184 2007 Fragments arranged by size: 2477 2007 890 224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2895 2952 3740 4099 4258 518 Size: 648 1729 57 788 359 159 2042 Fragments arranged by size: 2042 1729 788 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 3198 3875 5023 5268 1606 Size: 1592 677 1148 245 2120 Fragments arranged by size: 2120 1592 1148 677 245 NarI GG'CG_CC Cuts at: 2347 2347 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NsiI A_TGCA'T Cuts at: 2731 2731 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2713 2713 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 2064 3352 2064 Size: 1288 4494 PstI C_TGCA'G Cuts at: 830 2542 3134 830 Size: 1712 592 3478 Fragments arranged by size: 3478 1712 592 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SacI G_AGCT'C Cuts at: 3049 3049 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SapI GCTCTTCn'nnn_ Cuts at: 2191 2191 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2538 3130 4265 4946 5137 826 Size: 219 1493 592 1135 681 191 1471 Fragments arranged by size: 1493 1471 1135 681 592 219 191 SfoI GGC'GCC Cuts at: 2348 2348 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 3886 4787 5049 820 Size: 197 926 795 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 3221 513 Size: 552 2156 3074 Fragments arranged by size: 3074 2156 552 SwaI ATTT'AAAT Cuts at: 2925 2925 Size: 5782 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2130 3809 3826 3979 4164 4609 325 Size: 1255 550 1679 17 153 185 445 1498 Fragments arranged by size: 1679 1498 1255 550 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2587 4028 5740 Size: 274 80 33 51 1443 610 1441 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1443 1441 610 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 2666 1316 Size: 1350 4432 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI XcmI