(Circular) (Six-base) MAPSORT of: pFN21AB4417.seq Check: 6019 from: 1 to: 6175 pFN21AB4417 6175 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2948 4238 5622 5890 1632 Size: 422 894 1290 1384 268 1917 Fragments arranged by size: 1917 1384 1290 894 422 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 2899 1166 Size: 1733 4442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2571 3356 1184 Size: 1387 785 4003 Fragments arranged by size: 4003 1387 785 AlwNI CAG_nnn'CTG Cuts at: 2032 2464 2542 2587 3027 3102 3918 5490 2032 Size: 432 78 45 440 75 816 1572 2717 Fragments arranged by size: 2717 1572 816 440 432 78 75 45 ApaI G_GGCC'C Cuts at: 2214 2517 2214 Size: 303 5872 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 3484 3710 3862 5971 1493 Size: 1991 226 152 2109 1697 Fragments arranged by size: 2109 1991 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2727 3500 1870 Size: 73 784 773 4545 Fragments arranged by size: 4545 784 773 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2006 2039 2006 Size: 33 6142 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2467 2555 3332 3496 Size: 325 217 627 251 429 88 777 164 Cuts at: 3496 4951 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 777 627 429 325 251 217 164 88 BanII G_rGCy'C Cuts at: 1391 2055 2161 2214 2421 2517 3494 1391 Size: 664 106 53 207 96 977 4072 Fragments arranged by size: 4072 977 664 207 106 96 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5204 BbvCI CC'TCA_GC Cuts at: 3439 3439 Size: 6175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2512 2775 2879 Size: 107 122 71 631 605 840 263 104 Cuts at: 2879 4786 136 Size: 1907 1525 Fragments arranged by size: 1907 1525 840 631 605 263 122 107 104 71 BglII A'GATC_T Cuts at: 2742 2844 6170 2742 Size: 102 3326 2747 Fragments arranged by size: 3326 2747 102 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 2214 2517 2560 3335 4236 5392 1790 Size: 424 303 43 775 901 1156 2573 Fragments arranged by size: 2573 1156 901 775 424 303 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3415 3906 4864 Size: 416 53 83 399 24 1998 491 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 1998 1753 958 491 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2904 3023 3404 4835 1239 Size: 1665 119 381 1431 2579 Fragments arranged by size: 2579 1665 1431 381 119 Bpu10I CC'TnA_GC Cuts at: 3439 3439 Size: 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2724 4838 882 Size: 617 1225 2114 2219 Fragments arranged by size: 2219 2114 1225 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2598 3075 3982 4364 Size: 53 83 186 936 1065 477 907 382 Cuts at: 4364 4999 275 Size: 635 1451 Fragments arranged by size: 1451 1065 936 907 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2357 2387 3395 3425 1178 Size: 30 165 30 954 30 1008 30 3928 Fragments arranged by size: 3928 1008 954 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2453 1186 Size: 877 390 4908 Fragments arranged by size: 4908 877 390 BseYI C'CCAG_C Cuts at: 2115 2224 2332 2427 2507 2517 2692 3102 5378 Size: 109 108 95 80 10 175 410 2276 Cuts at: 5378 5874 2115 Size: 496 2416 Fragments arranged by size: 2416 2276 496 410 175 109 108 95 80 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5602 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4236 4321 5392 4236 Size: 85 1071 5019 Fragments arranged by size: 5019 1071 85 BsmI GAATG_Cn' Cuts at: 2268 3686 3779 2268 Size: 1418 93 4664 Fragments arranged by size: 4664 1418 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3317 3317 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2161 2214 2421 2517 2560 3335 Size: 399 265 106 53 207 96 43 775 Cuts at: 3335 3494 4236 4321 5392 1391 Size: 159 742 85 1071 2174 Fragments arranged by size: 2174 1071 775 742 399 265 207 159 106 96 85 53 43 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 2982 4064 5794 2982 Size: 1082 1730 3363 Fragments arranged by size: 3363 1730 1082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 3000 3530 844 Size: 1697 459 530 3489 Fragments arranged by size: 3489 1697 530 459 BsrBI CCG'CTC Cuts at: 1394 1823 2553 4062 1394 Size: 429 730 1509 3507 Fragments arranged by size: 3507 1509 730 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4670 4844 65 Size: 1183 3422 174 1396 Fragments arranged by size: 3422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2345 4819 5006 1700 Size: 110 535 2474 187 2869 Fragments arranged by size: 2869 2474 535 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 2542 3462 794 Size: 1748 920 3507 Fragments arranged by size: 3507 1748 920 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstXI CCAn_nnnn'nTGG Cuts at: 2796 2796 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2742 2844 3505 3872 4257 4274 5726 5812 Size: 770 102 661 367 385 17 1452 86 Cuts at: 5812 5824 6170 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 770 661 385 367 346 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 3398 2274 Size: 1124 5051 BtgI C'CryG_G Cuts at: 513 1065 2301 2593 3207 513 Size: 552 1236 292 614 3481 Fragments arranged by size: 3481 1236 614 552 292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2353 2476 487 Size: 683 56 1127 123 4186 Fragments arranged by size: 4186 1127 683 123 56 BtsI GCAGTG_nn' Cuts at: 2259 2656 3691 4485 4505 2259 Size: 397 1035 794 20 3929 Fragments arranged by size: 3929 1035 794 397 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2911 3622 4511 Size: 54 1425 50 275 18 1081 711 889 Cuts at: 4511 6140 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1081 889 711 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4105 1353 Size: 2752 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2543 4762 5136 5282 387 Size: 1064 138 954 2219 374 146 1280 Fragments arranged by size: 2219 1280 1064 954 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2904 2948 3023 3404 4238 4835 Size: 393 422 850 44 75 381 834 597 Cuts at: 4835 5622 5890 1239 Size: 787 268 1524 Fragments arranged by size: 1524 850 834 787 597 422 393 381 268 75 44 EcoNI CCTnn'n_nnAGG Cuts at: 2768 2768 Size: 6175 EcoO109I rG'GnC_Cy Cuts at: 2119 2278 2443 2514 2119 Size: 159 165 71 5780 Fragments arranged by size: 5780 165 159 71 EcoRI G'AATT_C Cuts at: 3484 3862 3484 Size: 378 5797 FspI TGC'GCA Cuts at: 2352 4681 2352 Size: 2329 3846 FspAI rTGC'GCAy Cuts at: 2352 2352 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 2224 5322 1678 Size: 546 3098 2531 Fragments arranged by size: 3098 2531 546 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2357 2389 3395 3427 4816 Size: 32 135 32 785 32 1006 32 1389 Cuts at: 4816 4848 4890 4922 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1389 1006 785 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3247 3519 3765 669 Size: 988 281 1309 272 246 3079 Fragments arranged by size: 3079 1309 988 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3321 5289 5473 1305 Size: 2016 1968 184 2007 Fragments arranged by size: 2016 2007 1968 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2899 3185 4133 4492 4651 518 Size: 648 1329 404 286 948 359 159 2042 Fragments arranged by size: 2042 1329 948 648 404 359 286 159 MspA1I CmG'CkG Cuts at: 1606 2115 2173 2455 2511 2718 3099 3209 3382 Size: 509 58 282 56 207 381 110 173 Cuts at: 3382 3443 3591 4268 5416 5661 1606 Size: 61 148 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 509 381 282 245 207 173 148 110 61 58 56 NcoI C'CATG_G Cuts at: 513 1065 2593 513 Size: 552 1528 4095 Fragments arranged by size: 4095 1528 552 NdeI CA'TA_TG Cuts at: 387 3235 387 Size: 2848 3327 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 2581 3533 5078 1132 Size: 97 1352 952 1545 2229 Fragments arranged by size: 2229 1545 1352 952 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2616 1108 Size: 1508 4667 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2851 2883 4244 4276 5757 5789 2851 Size: 32 1361 32 1481 32 3237 Fragments arranged by size: 3237 1481 1361 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2443 2119 Size: 324 5851 PshAI GACnn'nnGTC Cuts at: 1613 2681 1613 Size: 1068 5107 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PspOMI G'GGCC_C Cuts at: 2210 2513 2210 Size: 303 5872 PstI C_TGCA'G Cuts at: 830 2047 2460 2538 2550 2717 3527 830 Size: 1217 413 78 12 167 810 3478 Fragments arranged by size: 3478 1217 810 413 167 78 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2173 2455 2718 3099 3382 3443 2173 Size: 282 263 381 283 61 4905 Fragments arranged by size: 4905 381 283 282 263 61 SacII CC_GC'GG Cuts at: 3210 3210 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 2538 3527 2538 Size: 989 5186 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2043 2456 2534 2546 2713 3523 4658 Size: 219 998 413 78 12 167 810 1135 Cuts at: 4658 5339 5530 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 998 810 681 413 219 191 167 78 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 2345 1700 Size: 645 5530 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2727 4279 5180 5442 820 Size: 197 926 784 1552 901 262 1553 Fragments arranged by size: 1553 1552 926 901 784 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 2327 2753 1517 Size: 810 426 4939 Fragments arranged by size: 4939 810 426 StyI C'CwwG_G Cuts at: 513 1065 2593 3614 513 Size: 552 1528 1021 3074 Fragments arranged by size: 3074 1528 1021 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 2608 4202 4219 4372 4557 5002 Size: 1255 840 188 1594 17 153 185 445 Cuts at: 5002 325 Size: 1498 Fragments arranged by size: 1594 1498 1255 840 445 188 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 3258 4421 Size: 274 80 33 51 1443 729 552 1163 Cuts at: 4421 6133 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1163 729 552 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2617 2617 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 2727 1943 Size: 784 5391 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 2268 4304 2268 Size: 2036 4139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI