Restriction map for
pFN21AB4392
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 648 GTAC GT^AC
AgeI 623 ACCGGT A^CCGGT
AlwNI 32 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 568 CCTGAGG CC^T[ACGT]AGG
Aor51HI 157 AGCGCT AGC^GCT
BalI 977 TGGCCA TGG^CCA
BanII 612 GAGCCC G[GA]GC[CT]^C
BbsI 269 GAAGAC GAAGAC(2/6)
BfaI 886 CTAG C^TAG
BglII 175 AGATCT A^GATCT
BlpI 541 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 541 GCTCAGC GC^T[ACGT]AGC
BpuAI 269 GAAGAC GAAGAC(2/6)
BsrFI 623 ACCGGT [GA]^CCGG[CT]
BsrI 645 CCAGT ACTGG(1/-1)
Bsu36I 568 CCTGAGG CC^T[ACGT]AGG
CelII 541 GCTCAGC GC^T[ACGT]AGC
CfoI 158 GCGC GCG^C
Cfr10I 623 ACCGGT [GA]^CCGG[CT]
EaeI 977 TGGCCA [CT]^GGCC[GA]
Eco47III 157 AGCGCT AGC^GCT
Eco81I 568 CCTGAGG CC^T[ACGT]AGG
EcoNI 51 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 597 CCATGG C^C[AT][AT]GG
HaeII 157 AGCGCT [GA]GCGC^[CT]
HhaI 158 GCGC GCG^C
HinP1I 158 GCGC G^CGC
MaeI 886 CTAG C^TAG
MluNI 977 TGGCCA TGG^CCA
MscI 977 TGGCCA TGG^CCA
MslI 437 CACTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 470 CAGCTG C[AC]G^C[GT]G
NcoI 597 CCATGG C^CATGG
PinAI 623 ACCGGT A^CCGGT
PstI 113 CTGCAG CTGCA^G
PvuII 470 CAGCTG CAG^CTG
RsaI 648 GTAC GT^AC
ScaI 647 AGTACT AGT^ACT
StyI 597 CCATGG C^C[AT][AT]GG
TfiI 679 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 287 GATCC GGATC(4/5)
1038 GGATC
BanI 231 GGCACC G^G[CT][GA]CC
756 GGTGCC
BciVI 320 GGATAC GTATCC(6/5)
936 GGATAC
BmyI 612 GAGCCC G[AGT]GC[ACT]^C
757 GTGCCC
BsaWI 317 ACCGGA [AT]^CCGG[AT]
623 ACCGGT
Bsp1286I 612 GAGCCC G[AGT]GC[ACT]^C
757 GTGCCC
BsrBI 455 GAGCGG CCGCTC(-3/-3)
1033 GAGCGG
DsaI 492 CCGTGG C^C[GA][CT]GG
597 CCATGG
PaeR7I 452 CTCGAG C^TCGAG
1060 CTCGAG
SfcI 113 CTGCAG C^T[GA][CT]AG
891 CTACAG
TaqI 453 TCGA T^CGA
1061 TCGA
TthHB8I 453 TCGA T^CGA
1061 TCGA
XhoI 452 CTCGAG C^TCGAG
1060 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvCI
BbvI BcnI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI BspMI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI TseI Tsp45I TspRI
XhoII XmaCI XmaI