Restriction map for
pFN21AB4392
(Circular) (Six-base) MAPSORT of: pFN21AB4392.seq Check: 9237 from: 1 to: 5773
pFN21AB4392 5773 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2721 2721
Size: 5773
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 3836 5220 5488 1632
Size: 945 1259 1384 268 1917
Fragments arranged by size:
1917 1384 1259 945 268
AfeI AGC'GCT
Cuts at: 2164 2164
Size: 5773
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 2628 4604 2628
Size: 1976 3797
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 2446 1166
Size: 1280 4493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 2042 3516 5088 2042
Size: 1474 1572 2727
Fragments arranged by size:
2727 1572 1474
ApaLI G'TGCA_C
Cuts at: 3830 4986 3830
Size: 1156 4617
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2124 2418 2457 2776 2832 2916
Size: 73 144 37 294 39 319 56 84
Cuts at: 2916 2946 2976 3065 3098 1870
Size: 30 30 89 33 4545
Fragments arranged by size:
4545 319 294 144 89 84 73 56
39 37 33 30 30
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2761 3094 4549 618
Size: 325 217 627 449 525 333 1455 1842
Fragments arranged by size:
1842 1455 627 525 449 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2621 3092 1391
Size: 1230 471 4072
Fragments arranged by size:
4072 1230 471
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2281 928
Size: 971 382 4420
Fragments arranged by size:
4420 971 382
BbvCI CC'TCA_GC
Cuts at: 2304 2736 2795 2304
Size: 432 59 5282
Fragments arranged by size:
5282 432 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2319 2935 3665 4875 2319
Size: 616 730 1210 3217
Fragments arranged by size:
3217 1210 730 616
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5773
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4384 136
Size: 107 122 71 631 605 2712 1525
Fragments arranged by size:
2712 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2180 5768 2180
Size: 3588 2185
BlpI GC'TnA_GC
Cuts at: 2547 3190 2547
Size: 643 5130
Bme1580I G_kGCm'C
Cuts at: 1790 2766 3834 4990 1790
Size: 976 1068 1156 2573
Fragments arranged by size:
2573 1156 1068 976
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2181 2991 2181
Size: 810 4963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 2755 3016 4433 1239
Size: 1394 122 261 1417 2579
Fragments arranged by size:
2579 1417 1394 261 122
Bpu10I CC'TnA_GC
Cuts at: 2304 2736 2795 2304
Size: 432 59 5282
Fragments arranged by size:
5282 432 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2855 3898 4763 5061 2318
Size: 537 1043 865 298 3030
Fragments arranged by size:
3030 1043 865 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2637 2871 2962 4436 882
Size: 617 1138 234 91 1474 2219
Fragments arranged by size:
2219 1474 1138 617 234 91
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3580 3962 4597 275
Size: 53 83 186 936 2047 382 635 1451
Fragments arranged by size:
2047 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2322 2628 4206 4604 4878 5025 5436 2322
Size: 306 1578 398 274 147 411 2659
Fragments arranged by size:
2659 1578 411 398 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5548
Fragments arranged by size:
5548 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2409 3017 3032 1186
Size: 1223 608 15 3927
Fragments arranged by size:
3927 1223 608 15
BseYI C'CCAG_C
Cuts at: 2035 2048 2399 2805 2824 2910 2932 2957 4976
Size: 13 351 406 19 86 22 25 2019
Cuts at: 4976 5472 2035
Size: 496 2336
Fragments arranged by size:
2336 2019 496 406 351 86 25 22
19 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5200
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3834 3919 4990 3834
Size: 85 1071 4617
Fragments arranged by size:
4617 1071 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2621 2766 3092 3834 3919 4990 1391
Size: 399 831 145 326 742 85 1071 2174
Fragments arranged by size:
2174 1071 831 742 399 326 145 85
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2203 2361 2721 3128 844
Size: 1359 158 360 407 3489
Fragments arranged by size:
3489 1359 407 360 158
BsrBI CCG'CTC
Cuts at: 1394 1823 2462 3040 3660 1394
Size: 429 639 578 620 3507
Fragments arranged by size:
3507 639 620 578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2628 4417 4604 1700
Size: 110 818 1789 187 2869
Fragments arranged by size:
2869 1789 818 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 3833 4845 3833
Size: 1012 4761
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5773
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 2180 2291 3043 3103 3470 3855 3872 5324
Size: 208 111 752 60 367 385 17 1452
Cuts at: 5324 5410 5422 5768 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 752 385 367 346 208 111
86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2574 2574
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 2497 2602 513
Size: 552 1432 105 3684
Fragments arranged by size:
3684 1432 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 2259 2703 3289 4083 4103 2259
Size: 444 586 794 20 3929
Fragments arranged by size:
3929 794 586 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 809
Size: 507 3464 1802
Fragments arranged by size:
3464 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2982 3220 4109
Size: 54 1425 50 275 18 1152 238 889
Cuts at: 4109 5738 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1152 889 275 238 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3703 1353
Size: 2350 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4360 4734 4880 387
Size: 1064 138 2771 374 146 1280
Fragments arranged by size:
2771 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2577 2633 2755 3016 3836 4433 5220
Size: 393 945 56 122 261 820 597 787
Cuts at: 5220 5488 1239
Size: 268 1524
Fragments arranged by size:
1524 945 820 787 597 393 268 261
122 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 5773
EcoO109I rG'GnC_Cy
Cuts at: 2140 2350 2732 2828 2140
Size: 210 382 96 5085
Fragments arranged by size:
5085 382 210 96
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 4279 4279
Size: 5773
HaeII r_GCGC'y
Cuts at: 1678 2166 4920 1678
Size: 488 2754 2531
Fragments arranged by size:
2754 2531 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4414 4446 4488 4520 1373
Size: 32 135 32 2842 32 42 32 2626
Fragments arranged by size:
2842 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2712 4887 5071 1305
Size: 1407 2175 184 2007
Fragments arranged by size:
2175 2007 1407 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2984 10
Size: 54 1425 343 1152 2799
Fragments arranged by size:
2799 1425 1152 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2446 3731 4090 4249 518
Size: 648 1280 1285 359 159 2042
Fragments arranged by size:
2042 1285 1280 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2477 3189 3866 5014 5259 1606
Size: 871 712 677 1148 245 2120
Fragments arranged by size:
2120 1148 871 712 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2602 513
Size: 552 1537 3684
Fragments arranged by size:
3684 1537 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5773
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2350 2732 2828 2350
Size: 382 96 5295
Fragments arranged by size:
5295 382 96
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspXI vC'TCGA_Gb
Cuts at: 2457 3065 2457
Size: 608 5165
PstI C_TGCA'G
Cuts at: 830 2122 3125 830
Size: 1292 1003 3478
Fragments arranged by size:
3478 1292 1003
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2477 2477
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SanDI GG'GwC_CC
Cuts at: 2828 2828
Size: 5773
SbfI CC_TGCA'GG
Cuts at: 3125 3125
Size: 5773
ScaI AGT'ACT
Cuts at: 2654 4021 5733 2654
Size: 1367 1712 2694
Fragments arranged by size:
2694 1712 1367
SfcI C'TryA_G
Cuts at: 826 1045 2118 2896 3121 4256 4937 5128 826
Size: 219 1073 778 225 1135 681 191 1471
Fragments arranged by size:
1471 1135 1073 778 681 225 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 2420 2778 2834 3100 1872
Size: 548 358 56 266 4545
Fragments arranged by size:
4545 548 358 266 56
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 2457 2870 3065 3877 4778
Size: 197 926 354 160 413 195 812 901
Cuts at: 4778 5040 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 812 413 354 262 197
195 160
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SrfI GCCC'GGGC
Cuts at: 2778 2778
Size: 5773
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2602 3212 513
Size: 552 1537 610 3074
Fragments arranged by size:
3074 1537 610 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2500 2687 3800 3817 3970 4155 4600
Size: 1255 920 187 1113 17 153 185 445
Cuts at: 4600 325
Size: 1498
Fragments arranged by size:
1498 1255 1113 920 445 187 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2652 4019 5731
Size: 274 80 33 51 1443 675 1367 1712
Cuts at: 5731 96
Size: 138
Fragments arranged by size:
1712 1443 1367 675 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 5733 5765 2684
Size: 32 3017 32 2692
Fragments arranged by size:
3017 2692 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XhoI C'TCGA_G
Cuts at: 1943 2457 3065 1943
Size: 514 608 4651
Fragments arranged by size:
4651 608 514
XmaI C'CCGG_G
Cuts at: 1870 2418 2776 2832 3098 1870
Size: 548 358 56 266 4545
Fragments arranged by size:
4545 548 358 266 56
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SwaI XcmI