(Circular) (Six-base) MAPSORT of: pFN21AB4334.seq Check: 4217 from: 1 to: 6124 pFN21AB4334 6124 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 3423 4251 4624 1563 Size: 1860 828 373 3063 Fragments arranged by size: 3063 1860 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2732 4187 5571 5839 1632 Size: 1100 1455 1384 268 1917 Fragments arranged by size: 1917 1455 1384 1100 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 2103 2925 5023 6000 1203 Size: 900 822 2098 977 1327 Fragments arranged by size: 2098 1327 977 900 822 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 2386 3355 4853 2386 Size: 969 1498 3657 Fragments arranged by size: 3657 1498 969 AleI CACnn'nnGTG Cuts at: 1166 2849 1166 Size: 1683 4441 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AlwNI CAG_nnn'CTG Cuts at: 3867 5439 3867 Size: 1572 4552 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 3172 3433 3659 3811 5920 1493 Size: 1679 261 226 152 2109 1697 Fragments arranged by size: 2109 1697 1679 261 226 152 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2662 2837 3228 3449 1870 Size: 73 153 566 175 391 221 4545 Fragments arranged by size: 4545 566 391 221 175 153 73 BamHI G'GATC_C Cuts at: 2307 3454 3821 2307 Size: 1147 367 4610 Fragments arranged by size: 4610 1147 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 2687 3445 4900 618 Size: 325 217 627 569 331 758 1455 1842 Fragments arranged by size: 1842 1455 758 627 569 331 325 217 BanII G_rGCy'C Cuts at: 1391 2463 2628 3443 1391 Size: 1072 165 815 4072 Fragments arranged by size: 4072 1072 815 165 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2423 2668 3284 3291 928 Size: 971 524 245 616 7 3761 Fragments arranged by size: 3761 971 616 524 245 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3323 3357 4315 4349 1903 Size: 34 1386 34 958 34 3678 Fragments arranged by size: 3678 1386 958 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2729 4016 5226 2729 Size: 1287 1210 3627 Fragments arranged by size: 3627 1287 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BfrBI ATG'CAT Cuts at: 2487 2816 2487 Size: 329 5795 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6119 6119 Size: 6124 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 2361 4185 5341 1790 Size: 571 1824 1156 2573 Fragments arranged by size: 2573 1824 1156 571 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3855 4813 442 Size: 416 53 83 399 24 2438 958 1753 Fragments arranged by size: 2438 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2852 3243 2852 Size: 391 5733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2990 4784 1239 Size: 1751 1794 2579 Fragments arranged by size: 2579 1794 1751 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4249 5114 5412 4249 Size: 865 298 4961 Fragments arranged by size: 4961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2665 2882 3151 4787 882 Size: 617 1166 217 269 1636 2219 Fragments arranged by size: 2219 1636 1166 617 269 217 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 2201 6118 2201 Size: 3917 2207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3931 4313 4948 275 Size: 53 83 186 936 2398 382 635 1451 Fragments arranged by size: 2398 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2606 4557 4955 5229 5376 5787 2606 Size: 1951 398 274 147 411 2943 Fragments arranged by size: 2943 1951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2394 2424 1178 Size: 30 165 30 991 30 4878 Fragments arranged by size: 4878 991 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3307 1186 Size: 2121 4003 BseYI C'CCAG_C Cuts at: 5327 5823 5327 Size: 496 5628 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2512 1163 Size: 573 776 4775 Fragments arranged by size: 4775 776 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2628 2652 4185 4270 5341 2628 Size: 24 1533 85 1071 3411 Fragments arranged by size: 3411 1533 1071 85 24 BsmI GAATG_Cn' Cuts at: 3346 3635 3728 3346 Size: 289 93 5742 Fragments arranged by size: 5742 289 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2114 2114 Size: 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2463 2628 2652 3443 4185 4270 Size: 399 571 102 165 24 791 742 85 Cuts at: 4270 5341 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 791 742 571 399 165 102 85 24 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3479 844 Size: 2635 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4619 4793 65 Size: 1183 3371 174 1396 Fragments arranged by size: 3371 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4768 4955 1700 Size: 110 2958 187 2869 Fragments arranged by size: 2958 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 2822 794 Size: 2028 4096 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstYI r'GATC_y Cuts at: 1972 2307 3454 3821 4206 4223 5675 5761 5773 Size: 335 1147 367 385 17 1452 86 12 Cuts at: 5773 6119 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1147 385 367 346 335 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2498 2498 Size: 6124 BtgI C'CryG_G Cuts at: 513 1065 2559 513 Size: 552 1494 4078 Fragments arranged by size: 4078 1494 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2194 3099 487 Size: 683 56 968 905 3512 Fragments arranged by size: 3512 968 905 683 56 BtsI GCAGTG_nn' Cuts at: 3640 4434 4454 3640 Size: 794 20 5310 Fragments arranged by size: 5310 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089 Size: 54 1425 50 275 18 1741 889 1629 Cuts at: 6089 8 Size: 43 Fragments arranged by size: 1741 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2717 2998 4054 1353 Size: 1364 281 1056 3423 Fragments arranged by size: 3423 1364 1056 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2626 2626 Size: 6124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2732 2990 4187 4784 5571 5839 1239 Size: 393 1100 258 1197 597 787 268 1524 Fragments arranged by size: 1524 1197 1100 787 597 393 268 258 EcoNI CCTnn'n_nnAGG Cuts at: 3231 3231 Size: 6124 EcoO109I rG'GnC_Cy Cuts at: 2011 2177 3008 2011 Size: 166 831 5127 Fragments arranged by size: 5127 831 166 EcoRI G'AATT_C Cuts at: 3172 3433 3811 3172 Size: 261 378 5485 Fragments arranged by size: 5485 378 261 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 5271 1678 Size: 3593 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 3341 3373 4765 Size: 32 135 32 800 32 937 32 1392 Cuts at: 4765 4797 4839 4871 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1392 937 800 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 2986 3111 3421 3468 3714 Size: 988 281 301 747 125 310 47 246 Cuts at: 3714 669 Size: 3079 Fragments arranged by size: 3079 988 747 310 301 281 246 125 47 HindIII A'AGCT_T Cuts at: 748 3026 748 Size: 2278 3846 HpaI GTT'AAC Cuts at: 3421 3714 3421 Size: 293 5831 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5238 5422 1305 Size: 3933 184 2007 Fragments arranged by size: 3933 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4302 Fragments arranged by size: 4302 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2439 2849 2924 4082 4441 4600 Size: 648 1056 217 410 75 1158 359 159 Cuts at: 4600 518 Size: 2042 Fragments arranged by size: 2042 1158 1056 648 410 359 217 159 75 MspA1I CmG'CkG Cuts at: 1606 3540 4217 5365 5610 1606 Size: 1934 677 1148 245 2120 Fragments arranged by size: 2120 1934 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5572 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NsiI A_TGCA'T Cuts at: 2489 2818 2489 Size: 329 5795 NspI r_CATG'y Cuts at: 1132 1229 2107 2487 2929 3226 3482 5027 1132 Size: 97 878 380 442 297 256 1545 2229 Fragments arranged by size: 2229 1545 878 442 380 297 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 2103 2925 5023 2103 Size: 822 2098 3204 Fragments arranged by size: 3204 2098 822 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PfoI T'CCnGG_A Cuts at: 3359 3359 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2177 2177 Size: 6124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2720 2752 2720 Size: 32 6092 PstI C_TGCA'G Cuts at: 830 3476 830 Size: 2646 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 SacI G_AGCT'C Cuts at: 2628 2628 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SanDI GG'GwC_CC Cuts at: 2177 2177 Size: 6124 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SfcI C'TryA_G Cuts at: 826 1045 3472 4607 5288 5479 826 Size: 219 2427 1135 681 191 1471 Fragments arranged by size: 2427 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 2098 3451 1872 Size: 226 1353 4545 Fragments arranged by size: 4545 1353 226 SmlI C'TyrA_G Cuts at: 820 1017 1943 2662 3228 4228 5129 5391 820 Size: 197 926 719 566 1000 901 262 1553 Fragments arranged by size: 1553 1000 926 901 719 566 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 2487 3226 3482 1132 Size: 1355 739 256 3774 Fragments arranged by size: 3774 1355 739 256 SspI AAT'ATT Cuts at: 5 52 2276 3103 4048 5 Size: 47 2224 827 945 2081 Fragments arranged by size: 2224 2081 945 827 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6124 StyI C'CwwG_G Cuts at: 513 1065 3045 3563 513 Size: 552 1980 518 3074 Fragments arranged by size: 3074 1980 552 518 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 2779 2891 4151 4168 4321 4506 Size: 1255 764 435 112 1260 17 153 185 Cuts at: 4506 4951 325 Size: 445 1498 Fragments arranged by size: 1498 1260 1255 764 445 435 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3207 3411 4370 Size: 274 80 33 51 1443 1230 204 959 Cuts at: 4370 6082 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1230 959 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2394 2426 6084 6116 2394 Size: 32 3658 32 2402 Fragments arranged by size: 3658 2402 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2221 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 2662 3228 1943 Size: 719 566 4839 Fragments arranged by size: 4839 719 566 XmaI C'CCGG_G Cuts at: 1870 2096 3449 1870 Size: 226 1353 4545 Fragments arranged by size: 4545 1353 226 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI