(Circular) (Six-base) MAPSORT of: pFN21AB4327.seq Check: 4840 from: 1 to: 6319 pFN21AB4327 6319 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2290 2290 Size: 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2520 3662 1937 Size: 583 1142 4594 Fragments arranged by size: 4594 1142 583 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4382 5766 6034 1632 Size: 2750 1384 268 1917 Fragments arranged by size: 2750 1917 1384 268 AfeI AGC'GCT Cuts at: 2125 2125 Size: 6319 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3494 3521 1184 Size: 2310 27 3982 Fragments arranged by size: 3982 2310 27 AlwNI CAG_nnn'CTG Cuts at: 2812 2845 4062 5634 2812 Size: 33 1217 1572 3497 Fragments arranged by size: 3497 1572 1217 33 ApaI G_GGCC'C Cuts at: 2423 2423 Size: 6319 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 3509 3644 1870 Size: 73 1566 135 4545 Fragments arranged by size: 4545 1566 135 73 BamHI G'GATC_C Cuts at: 2775 3649 4016 2775 Size: 874 367 5078 Fragments arranged by size: 5078 874 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 3041 3640 5095 618 Size: 325 217 627 245 1009 599 1455 1842 Fragments arranged by size: 1842 1455 1009 627 599 325 245 217 BanII G_rGCy'C Cuts at: 1391 2423 3005 3199 3264 3638 1391 Size: 1032 582 194 65 374 4072 Fragments arranged by size: 4072 1032 582 374 194 65 BbeI G_GCGC'C Cuts at: 2036 2036 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3055 928 Size: 971 1156 4192 Fragments arranged by size: 4192 1156 971 BbvCI CC'TCA_GC Cuts at: 2771 3290 3309 2771 Size: 519 19 5781 Fragments arranged by size: 5781 519 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2170 2204 4510 4544 1903 Size: 34 233 34 2306 34 3678 Fragments arranged by size: 3678 2306 233 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 2661 3351 1548 Size: 1113 690 4516 Fragments arranged by size: 4516 1113 690 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2803 2831 3099 Size: 107 122 71 631 605 1131 28 268 Cuts at: 3099 4930 136 Size: 1831 1525 Fragments arranged by size: 1831 1525 1131 631 605 268 122 107 71 28 BglII A'GATC_T Cuts at: 3220 6314 3220 Size: 3094 3225 BlpI GC'TnA_GC Cuts at: 2784 3736 2784 Size: 952 5367 Bme1580I G_kGCm'C Cuts at: 1790 2423 2604 4380 5536 1790 Size: 633 181 1776 1156 2573 Fragments arranged by size: 2573 1776 1156 633 181 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4050 5008 442 Size: 416 53 83 399 24 2633 958 1753 Fragments arranged by size: 2633 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 2555 2735 2867 4979 1239 Size: 1091 225 180 132 2112 2579 Fragments arranged by size: 2579 2112 1091 225 180 132 Bpu10I CC'TnA_GC Cuts at: 2771 3290 3309 2771 Size: 519 19 5781 Fragments arranged by size: 5781 519 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 4444 5309 5607 2201 Size: 2243 865 298 2913 Fragments arranged by size: 2913 2243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3482 4982 882 Size: 617 1983 1500 2219 Fragments arranged by size: 2219 1983 1500 617 BsaAI yAC'GTr Cuts at: 493 1204 3433 4110 493 Size: 711 2229 677 2702 Fragments arranged by size: 2702 2229 711 677 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2109 2463 4126 Size: 53 83 186 936 500 76 354 1663 Cuts at: 4126 4508 5143 275 Size: 382 635 1451 Fragments arranged by size: 1663 1451 936 635 500 382 354 186 83 76 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2747 2777 1178 Size: 30 165 30 1344 30 4720 Fragments arranged by size: 4720 1344 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2443 2768 2834 2888 3006 3009 3259 3446 Size: 1257 325 66 54 118 3 250 187 Cuts at: 3446 1186 Size: 4059 Fragments arranged by size: 4059 1257 325 250 187 118 66 54 3 BseYI C'CCAG_C Cuts at: 2082 2812 3151 3393 5522 6018 2082 Size: 730 339 242 2129 496 2383 Fragments arranged by size: 2383 2129 730 496 339 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2200 1163 Size: 573 464 5282 Fragments arranged by size: 5282 573 464 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 3026 3519 4380 4465 5536 2250 Size: 776 493 861 85 1071 3033 Fragments arranged by size: 3033 1071 861 776 493 85 BsmI GAATG_Cn' Cuts at: 3462 3830 3923 3462 Size: 368 93 5858 Fragments arranged by size: 5858 368 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2423 2604 3005 3026 3199 3264 Size: 399 460 173 181 401 21 173 65 Cuts at: 3264 3519 3638 4380 4465 5536 1391 Size: 255 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 401 399 255 181 173 173 119 85 65 21 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 2864 3674 844 Size: 1446 574 810 3489 Fragments arranged by size: 3489 1446 810 574 BsrBI CCG'CTC Cuts at: 1394 1823 2669 3384 4206 1394 Size: 429 846 715 822 3507 Fragments arranged by size: 3507 846 822 715 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2044 2209 2952 3132 4814 4988 65 Size: 1183 796 165 743 180 1682 174 1396 Fragments arranged by size: 1682 1396 1183 796 743 180 174 165 BsrFI r'CCGG_y Cuts at: 1700 1810 2501 2793 3128 3540 4963 5150 1700 Size: 110 691 292 335 412 1423 187 2869 Fragments arranged by size: 2869 1423 691 412 335 292 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 2176 4379 5391 2176 Size: 2203 1012 3104 Fragments arranged by size: 3104 2203 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3436 794 Size: 2642 3677 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 2360 3073 1184 Size: 1176 713 4430 Fragments arranged by size: 4430 1176 713 BstYI r'GATC_y Cuts at: 1972 2275 2316 2775 3220 3649 4016 4401 4418 Size: 303 41 459 445 429 367 385 17 Cuts at: 4418 5870 5956 5968 6314 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 459 445 429 385 367 346 303 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2800 3078 3337 3412 513 Size: 552 939 796 278 259 75 3420 Fragments arranged by size: 3420 939 796 552 278 259 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3835 4629 4649 2971 Size: 864 794 20 4641 Fragments arranged by size: 4641 864 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 3192 3436 3192 Size: 244 6075 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2516 5326 809 Size: 507 1200 2810 1802 Fragments arranged by size: 2810 1802 1200 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2266 2380 3033 Size: 54 1425 50 275 18 436 114 653 Cuts at: 3033 3091 3766 4655 6284 8 Size: 58 675 889 1629 43 Fragments arranged by size: 1629 1425 889 675 653 436 275 114 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2327 2687 3269 4249 1353 Size: 974 360 582 980 3423 Fragments arranged by size: 3423 980 974 582 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2555 2735 2867 4382 4979 5766 Size: 393 698 225 180 132 1515 597 787 Cuts at: 5766 6034 1239 Size: 268 1524 Fragments arranged by size: 1524 1515 787 698 597 393 268 225 180 132 EcoNI CCTnn'n_nnAGG Cuts at: 2988 3509 2988 Size: 521 5798 EcoO109I rG'GnC_Cy Cuts at: 2058 2420 2833 2842 2058 Size: 362 413 9 5535 Fragments arranged by size: 5535 413 362 9 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2036 2127 2145 3371 3400 5466 1678 Size: 358 91 18 1226 29 2066 2531 Fragments arranged by size: 2531 2066 1226 358 91 29 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2653 2685 2747 2779 3343 Size: 32 135 32 1081 32 62 32 564 Cuts at: 3343 3375 4960 4992 5034 5066 1373 Size: 32 1585 32 42 32 2626 Fragments arranged by size: 2626 1585 1081 564 135 62 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2509 3663 3909 669 Size: 988 281 571 1154 246 3079 Fragments arranged by size: 3079 1154 988 571 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 2032 2032 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3035 10 Size: 54 1425 343 1203 3294 Fragments arranged by size: 3294 1425 1203 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4277 4636 4795 518 Size: 648 3111 359 159 2042 Fragments arranged by size: 3111 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2106 2184 3039 3576 3592 3735 4412 Size: 435 65 78 855 537 16 143 677 Cuts at: 4412 5560 5805 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 855 677 537 435 245 143 78 65 16 NaeI GCC'GGC Cuts at: 3542 3542 Size: 6319 NarI GG'CG_CC Cuts at: 2033 2033 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2004 2800 3412 513 Size: 552 939 796 612 3420 Fragments arranged by size: 3420 939 796 612 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 3540 3540 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3677 5222 1132 Size: 97 2448 1545 2229 Fragments arranged by size: 2448 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 3192 1108 Size: 1878 206 4235 Fragments arranged by size: 4235 1878 206 PfoI T'CCnGG_A Cuts at: 2307 2712 3519 2307 Size: 405 807 5107 Fragments arranged by size: 5107 807 405 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 3433 3433 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2842 2842 Size: 6319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2419 2419 Size: 6319 PstI C_TGCA'G Cuts at: 830 2449 2841 3429 3671 830 Size: 1619 392 588 242 3478 Fragments arranged by size: 3478 1619 588 392 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SapI GCTCTTCn'nnn_ Cuts at: 3269 3269 Size: 6319 SbfI CC_TGCA'GG Cuts at: 2841 3671 2841 Size: 830 5489 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 2445 2651 2837 3425 3667 4802 5483 Size: 219 1400 206 186 588 242 1135 681 Cuts at: 5483 5674 826 Size: 191 1471 Fragments arranged by size: 1471 1400 1135 681 588 242 219 206 191 186 SfiI GGCCn_nnn'nGGCC Cuts at: 2831 2831 Size: 6319 SfoI GGC'GCC Cuts at: 2034 2034 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 4423 5324 5586 820 Size: 197 926 273 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 273 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 2800 3256 3412 3758 513 Size: 552 939 402 394 456 156 346 3074 Fragments arranged by size: 3074 939 552 456 402 394 346 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 3099 4346 4363 4516 4701 5146 Size: 1255 924 595 1247 17 153 185 445 Cuts at: 5146 325 Size: 1498 Fragments arranged by size: 1498 1255 1247 924 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4565 6277 96 Size: 274 80 33 51 1443 2588 1712 138 Fragments arranged by size: 2588 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 6279 6311 2267 Size: 32 3980 32 2275 Fragments arranged by size: 3980 2275 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 2464 3410 2026 Size: 438 946 4935 Fragments arranged by size: 4935 946 438 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI