(Circular) (Six-base) MAPSORT of: pFN21AB4286.seq Check: 3438 from: 1 to: 5410 pFN21AB4286 5410 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3220 278 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2731 2731 Size: 5410 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2284 2485 3473 4857 5125 1632 Size: 512 140 201 988 1384 268 1917 Fragments arranged by size: 1917 1384 988 512 268 201 140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 4309 5286 1203 Size: 3106 977 1327 Fragments arranged by size: 3106 1327 977 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 4139 4139 Size: 5410 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5410 AlwNI CAG_nnn'CTG Cuts at: 3153 4725 3153 Size: 1572 3838 ApaLI G'TGCA_C Cuts at: 3467 4623 3467 Size: 1156 4254 ApoI r'AATT_y Cuts at: 1493 2455 2719 2945 3097 5206 1493 Size: 962 264 226 152 2109 1697 Fragments arranged by size: 2109 1697 962 264 226 152 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2735 1870 Size: 73 792 4545 Fragments arranged by size: 4545 792 73 BamHI G'GATC_C Cuts at: 2691 2740 3107 2691 Size: 49 367 4994 Fragments arranged by size: 4994 367 49 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2731 4186 618 Size: 325 217 627 944 1455 1842 Fragments arranged by size: 1842 1455 944 627 325 217 BanII G_rGCy'C Cuts at: 1391 2729 1391 Size: 1338 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3601 3635 1903 Size: 34 1664 34 3678 Fragments arranged by size: 3678 1664 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3302 4512 3302 Size: 1210 4200 BclI T'GATC_A Cuts at: 1548 1548 Size: 5410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4021 136 Size: 107 122 71 631 605 2349 1525 Fragments arranged by size: 2349 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5405 5405 Size: 5410 BlpI GC'TnA_GC Cuts at: 2827 2827 Size: 5410 Bme1580I G_kGCm'C Cuts at: 1790 3471 4627 1790 Size: 1681 1156 2573 Fragments arranged by size: 2573 1681 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3141 4099 442 Size: 416 53 83 399 24 1724 958 1753 Fragments arranged by size: 1753 1724 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2165 2165 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 4070 1239 Size: 1091 1740 2579 Fragments arranged by size: 2579 1740 1091 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4400 4698 3535 Size: 865 298 4247 Fragments arranged by size: 4247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4073 882 Size: 617 2574 2219 Fragments arranged by size: 2574 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3201 493 Size: 711 1997 2702 Fragments arranged by size: 2702 1997 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3217 3599 4234 275 Size: 53 83 186 936 1684 382 635 1451 Fragments arranged by size: 1684 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5185 Fragments arranged by size: 5185 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2378 1186 Size: 1189 3 4218 Fragments arranged by size: 4218 1189 3 BseYI C'CCAG_C Cuts at: 2197 2627 4613 5109 2197 Size: 430 1986 496 2498 Fragments arranged by size: 2498 1986 496 430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4837 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2860 3621 4649 1540 Size: 275 186 859 761 1028 2301 Fragments arranged by size: 2301 1028 859 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 2432 3471 3556 4627 2177 Size: 255 1039 85 1071 2960 Fragments arranged by size: 2960 1071 1039 255 85 BsmI GAATG_Cn' Cuts at: 2272 2921 3014 2272 Size: 649 93 4668 Fragments arranged by size: 4668 649 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2432 2729 3471 3556 4627 1391 Size: 399 387 255 297 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 297 255 85 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 2181 3299 5029 2181 Size: 1118 1730 2562 Fragments arranged by size: 2562 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2765 844 Size: 1921 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3297 1394 Size: 429 1474 3507 Fragments arranged by size: 3507 1474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3905 4079 65 Size: 1183 2657 174 1396 Fragments arranged by size: 2657 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4054 4241 1700 Size: 110 2244 187 2869 Fragments arranged by size: 2869 2244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5410 BssSI C'ACGA_G Cuts at: 3470 4482 3470 Size: 1012 4398 BstAPI GCAn_nnn'nTGC Cuts at: 794 2645 794 Size: 1851 3559 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstXI CCAn_nnnn'nTGG Cuts at: 2319 2476 2319 Size: 157 5253 BstYI r'GATC_y Cuts at: 1972 2691 2740 3107 3492 3509 4961 5047 5059 Size: 719 49 367 385 17 1452 86 12 Cuts at: 5059 5405 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 719 385 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2649 513 Size: 552 1584 3274 Fragments arranged by size: 3274 1584 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4671 Fragments arranged by size: 4671 683 56 BtsI GCAGTG_nn' Cuts at: 2926 3720 3740 2926 Size: 794 20 4596 Fragments arranged by size: 4596 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2245 4417 809 Size: 507 929 2172 1802 Fragments arranged by size: 2172 1802 929 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2609 2857 Size: 54 1425 50 275 18 193 586 248 Cuts at: 2857 3746 5375 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 586 275 248 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2857 1537 Size: 275 1045 4090 Fragments arranged by size: 4090 1045 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2038 2238 2397 2546 2609 3340 1353 Size: 685 200 159 149 63 731 3423 Fragments arranged by size: 3423 731 685 200 159 149 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3997 4371 4517 387 Size: 1064 138 2408 374 146 1280 Fragments arranged by size: 2408 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2284 2330 2485 3473 4070 4857 Size: 393 512 140 46 155 988 597 787 Cuts at: 4857 5125 1239 Size: 268 1524 Fragments arranged by size: 1524 988 787 597 512 393 268 155 140 46 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 FspI TGC'GCA Cuts at: 3916 3916 Size: 5410 HaeII r_GCGC'y Cuts at: 1678 4557 1678 Size: 2879 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4051 4083 4125 4157 1373 Size: 32 135 32 2479 32 42 32 2626 Fragments arranged by size: 2626 2479 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 2754 3000 669 Size: 988 281 421 395 246 3079 Fragments arranged by size: 3079 988 421 395 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5410 HpaI GTT'AAC Cuts at: 2359 3000 2359 Size: 641 4769 KpnI G_GTAC'C Cuts at: 2735 2735 Size: 5410 MfeI C'AATT_G Cuts at: 2640 3009 2640 Size: 369 5041 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 2719 4524 4708 1305 Size: 1302 112 1805 184 2007 Fragments arranged by size: 2007 1805 1302 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 2611 10 Size: 54 1425 343 779 2809 Fragments arranged by size: 2809 1425 779 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 3368 3727 3886 518 Size: 648 1017 1185 359 159 2042 Fragments arranged by size: 2042 1185 1017 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2304 2826 3503 4651 4896 1606 Size: 698 522 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 522 245 NcoI C'CATG_G Cuts at: 513 1065 2649 513 Size: 552 1584 3274 Fragments arranged by size: 3274 1584 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2857 2857 Size: 5410 NspI r_CATG'y Cuts at: 1132 1229 2227 2768 4313 1132 Size: 97 998 541 1545 2229 Fragments arranged by size: 2229 1545 998 541 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5410 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5410 PfoI T'CCnGG_A Cuts at: 2094 2094 Size: 5410 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PstI C_TGCA'G Cuts at: 830 2762 830 Size: 1932 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 PvuII CAG'CTG Cuts at: 2304 2304 Size: 5410 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SbfI CC_TGCA'GG Cuts at: 2762 2762 Size: 5410 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SfcI C'TryA_G Cuts at: 826 1045 2575 2758 3893 4574 4765 826 Size: 219 1530 183 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 681 219 191 183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2737 1872 Size: 865 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3514 4415 4677 820 Size: 197 926 1571 901 262 1553 Fragments arranged by size: 1571 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SspI AAT'ATT Cuts at: 5 52 2065 3334 5 Size: 47 2013 1269 2081 Fragments arranged by size: 2081 2013 1269 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5410 StyI C'CwwG_G Cuts at: 513 1065 2649 2849 513 Size: 552 1584 200 3074 Fragments arranged by size: 3074 1584 552 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2154 2255 3437 3454 3607 3792 4237 Size: 1255 574 101 1182 17 153 185 445 Cuts at: 4237 325 Size: 1498 Fragments arranged by size: 1498 1255 1182 574 445 185 153 101 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3656 5368 96 Size: 274 80 33 51 1443 1679 1712 138 Fragments arranged by size: 1712 1679 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5370 5402 5370 Size: 32 5378 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5410 XmaI C'CCGG_G Cuts at: 1870 2735 1870 Size: 865 4545 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3218 276 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI