Restriction map for
pFN21AB4266
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbrPI
BbvCI BciVI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PstI PvuI RcaI RsrII
SacI SalI SbfI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AclI 676 AACGTT AA^CGTT
AcsI 1481 AAATTT [GA]^AATT[CT]
AlwI 1399 GGATC GGATC(4/5)
Aor51HI 638 AGCGCT AGC^GCT
ApoI 1481 AAATTT [GA]^AATT[CT]
Asp700I 1308 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1227 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 423 CTCGGG C^[CT]CG[GA]G
BalI 851 TGGCCA TGG^CCA
BanI 506 GGCGCC G^G[CT][GA]CC
BbeI 506 GGCGCC GGCGC^C
BbsI 187 GTCTTC GAAGAC(2/6)
BcgI 92 GCACGCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 32 TGATCA T^GATCA
BfaI 1019 CTAG C^TAG
BpuAI 187 GTCTTC GAAGAC(2/6)
BsgI 226 CTGCAC GTGCAG(16/14)
BsiEI 171 CGACCG CG[GA][CT]^CG
BsmBI 718 GAGACG CGTCTC(1/5)
BsoBI 423 CTCGGG C^[CT]CG[GA]G
BspMI 1501 GCAGGT ACCTGC(4/8)
BsrDI 683 GCAATG GCAATG(2/0)
BsrFI 488 GCCGGC [GA]^CCGG[CT]
BsrI 1133 CCAGT ACTGG(1/-1)
BssSI 634 CACGAG CACGAG(-5/-1)
BstEII 1185 GGTCACC G^GT[ACGT]ACC
BstPI 1185 GGTCACC G^GT[ACGT]ACC
BstXI 455 CCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1399 GGATCT [GA]^GATC[CT]
Cfr10I 488 GCCGGC [GA]^CCGG[CT]
DraIII 979 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 638 AGCGCT AGC^GCT
EcoNI 1513 CCTCTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1185 GGTCACC G^GT[ACGT]ACC
FbaI 32 TGATCA T^GATCA
FseI 487 GGCCGGCC GGCCGG^CC
KasI 506 GGCGCC G^GCGCC
KspI 1381 CCGCGG CCGC^GG
MaeI 1019 CTAG C^TAG
MflI 1399 GGATCT [GA]^GATC[CT]
MluNI 851 TGGCCA TGG^CCA
MscI 851 TGGCCA TGG^CCA
NaeI 488 GCCGGC GCC^GGC
NarI 506 GGCGCC GG^CGCC
NcoI 1337 CCATGG C^CATGG
NgoMIV 488 GCCGGC G^CCGGC
NheI 1018 GCTAGC G^CTAGC
PflFI 1227 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 978 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1456 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 676 AACGTT AA^CGTT
PvuII 1393 CAGCTG CAG^CTG
SacII 1381 CCGCGG CCGC^GG
SapI 534 GCTCTTC GCTCTTC(1/4)
ScaI 1444 AGTACT AGT^ACT
SfoI 506 GGCGCC GGC^GCC
SphI 1238 GCATGC GCATG^C
StuI 725 AGGCCT AGG^CCT
Tth111I 1227 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 978 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1399 GGATCT [GA]^GATC[CT]
XmnI 1308 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 1138 GAGCAC G[AT]GC[AT]^C
1273 GTGCTC
BlpI 882 GCTGAGC GC^T[ACGT]AGC
1342 GCTAAGC
Bpu1102I 882 GCTGAGC GC^T[ACGT]AGC
1342 GCTAAGC
BsiHKAI 1138 GAGCAC G[AT]GC[AT]^C
1273 GTGCTC
BsmFI 11 GGGAC GGGAC(10/14)
555 GTCCC
BspLU11I 1214 ACATGT A^CATGT
1459 ACATGT
CelII 882 GCTGAGC GC^T[ACGT]AGC
1342 GCTAAGC
DraII 542 AGGACCC [GA]G^G[ACGT]CC[CT]
995 GGGTCCT
EarI 535 CTCTTC CTCTTC(1/4)
589 CTCTTC
EcoO109I 542 AGGACCC [GA]G^G[ACGT]CC[CT]
995 GGGTCCT
HinfI 421 GACTC G^A[ACGT]TC
475 GACTC
Ksp632I 535 CTCTTC CTCTTC(1/4)
589 CTCTTC
PleI 421 GACTC GAGTC(4/5)
475 GACTC
PpuMI 542 AGGACCC [GA]G^G[AT]CC[CT]
995 GGGTCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AvaII AviII BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrBI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI