(Circular) (Six-base) MAPSORT of: pFN21AB4266.seq Check: 6888 from: 1 to: 6238 pFN21AB4266 6238 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 2682 4365 4738 1563 Size: 1119 1683 373 3063 Fragments arranged by size: 3063 1683 1119 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4301 5685 5953 1632 Size: 2669 1384 268 1917 Fragments arranged by size: 2669 1917 1384 268 AfeI AGC'GCT Cuts at: 2645 2645 Size: 6238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 2247 3219 3464 5137 6114 1203 Size: 1044 972 245 1673 977 1327 Fragments arranged by size: 1673 1327 1044 977 972 245 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 2699 1166 Size: 1533 4705 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2717 1184 Size: 1533 4705 AlwNI CAG_nnn'CTG Cuts at: 3981 5553 3981 Size: 1572 4666 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 3486 3547 3773 3925 6034 1493 Size: 1993 61 226 152 2109 1697 Fragments arranged by size: 2109 1993 1697 226 152 61 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2428 3563 1870 Size: 73 485 1135 4545 Fragments arranged by size: 4545 1135 485 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2511 3559 5014 618 Size: 325 217 627 724 1048 1455 1842 Fragments arranged by size: 1842 1455 1048 724 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 2313 3259 3557 1391 Size: 733 189 946 298 4072 Fragments arranged by size: 4072 946 733 298 189 BbeI G_GCGC'C Cuts at: 2515 2515 Size: 6238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2185 928 Size: 971 286 4981 Fragments arranged by size: 4981 971 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2086 2120 3517 3551 4429 4463 1903 Size: 34 149 34 1397 34 878 34 3678 Fragments arranged by size: 3678 1397 878 149 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2114 2187 2354 Size: 107 122 71 631 605 442 73 167 Cuts at: 2354 2499 4849 136 Size: 145 2350 1525 Fragments arranged by size: 2350 1525 631 605 442 167 145 122 107 73 71 BglII A'GATC_T Cuts at: 6233 6233 Size: 6238 BlpI GC'TnA_GC Cuts at: 2888 3348 3655 2888 Size: 460 307 5471 Fragments arranged by size: 5471 460 307 Bme1580I G_kGCm'C Cuts at: 1790 3118 3303 4299 5455 1790 Size: 1328 185 996 1156 2573 Fragments arranged by size: 2573 1328 1156 996 185 BmgBI CAC'GTC Cuts at: 1318 2248 2305 2920 3234 1318 Size: 930 57 615 314 4322 Fragments arranged by size: 4322 930 615 314 57 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3969 4927 442 Size: 416 53 83 399 24 2552 958 1753 Fragments arranged by size: 2552 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3027 1056 Size: 1971 4267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 2651 2897 4898 1239 Size: 1232 180 246 2001 2579 Fragments arranged by size: 2579 2001 1232 246 180 Bpu10I CC'TnA_GC Cuts at: 3337 3337 Size: 6238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 2614 2627 4901 882 Size: 617 792 323 13 2274 2219 Fragments arranged by size: 2274 2219 792 617 323 13 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2190 2346 2409 Size: 53 83 186 936 594 63 156 63 Cuts at: 2409 2512 2754 4045 4427 5062 275 Size: 103 242 1291 382 635 1451 Fragments arranged by size: 1451 1291 936 635 594 382 242 186 156 103 83 63 63 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6013 Fragments arranged by size: 6013 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2285 2366 2370 1186 Size: 1096 3 81 4 5054 Fragments arranged by size: 5054 1096 81 4 3 BseYI C'CCAG_C Cuts at: 2239 2923 3131 3400 5441 5937 2239 Size: 684 208 269 2041 496 2540 Fragments arranged by size: 2540 2041 684 496 269 208 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 1163 Size: 573 480 5185 Fragments arranged by size: 5185 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2179 3537 3688 4449 5477 1540 Size: 275 186 178 1358 151 761 1028 2301 Fragments arranged by size: 2301 1358 1028 761 275 186 178 151 BsiHKAI G_wGCw'C Cuts at: 3147 3282 4299 4384 5455 3147 Size: 135 1017 85 1071 3930 Fragments arranged by size: 3930 1071 1017 135 85 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 BsmBI CGTCTCn'nnnn_ Cuts at: 2717 2717 Size: 6238 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2313 3118 3147 3259 3282 3303 Size: 399 334 189 805 29 112 23 21 Cuts at: 3303 3557 4299 4384 5455 1391 Size: 254 742 85 1071 2174 Fragments arranged by size: 2174 1071 805 742 399 334 254 189 112 85 29 23 21 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 3593 844 Size: 2653 96 3489 Fragments arranged by size: 3489 2653 96 BsrBI CCG'CTC Cuts at: 1394 1823 2073 2260 2892 4125 1394 Size: 429 250 187 632 1233 3507 Fragments arranged by size: 3507 1233 632 429 250 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2695 4733 4907 65 Size: 1183 1447 2038 174 1396 Fragments arranged by size: 2038 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2493 4882 5069 1700 Size: 110 683 2389 187 2869 Fragments arranged by size: 2869 2389 683 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 2639 4298 5310 2639 Size: 1659 1012 3567 Fragments arranged by size: 3567 1659 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 3190 1184 Size: 2006 4232 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2467 Size: 6238 BstYI r'GATC_y Cuts at: 1972 3404 3568 3935 4320 4337 5789 5875 5887 Size: 1432 164 367 385 17 1452 86 12 Cuts at: 5887 6233 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1432 385 367 346 164 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3186 3342 3386 513 Size: 552 939 1182 156 44 3365 Fragments arranged by size: 3365 1182 939 552 156 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3271 487 Size: 683 56 2045 3454 Fragments arranged by size: 3454 2045 683 56 BtsI GCAGTG_nn' Cuts at: 2233 3754 4548 4568 2233 Size: 1521 794 20 3903 Fragments arranged by size: 3903 1521 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DraIII CAC_nnn'GTG Cuts at: 2989 2989 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2491 2856 3389 Size: 54 1425 50 275 18 661 365 533 Cuts at: 3389 3685 4574 6203 8 Size: 296 889 1629 43 Fragments arranged by size: 1629 1425 889 661 533 365 296 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2546 2600 4168 1353 Size: 1193 54 1568 3423 Fragments arranged by size: 3423 1568 1193 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2132 4825 5199 5345 387 Size: 1064 138 543 2693 374 146 1280 Fragments arranged by size: 2693 1280 1064 543 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2651 2897 4301 4898 5685 5953 Size: 393 839 180 246 1404 597 787 268 Cuts at: 5953 1239 Size: 1524 Fragments arranged by size: 1524 1404 839 787 597 393 268 246 180 EcoNI CCTnn'n_nnAGG Cuts at: 3522 3522 Size: 6238 EcoO109I rG'GnC_Cy Cuts at: 2548 3001 2548 Size: 453 5785 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 FseI GG_CCGG'CC Cuts at: 2497 2497 Size: 6238 FspI TGC'GCA Cuts at: 2084 2954 2963 4744 2084 Size: 870 9 1781 3578 Fragments arranged by size: 3578 1781 870 9 FspAI rTGC'GCAy Cuts at: 2954 2954 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2379 2397 2467 2474 2515 2540 2647 3138 Size: 701 18 70 7 41 25 107 491 Cuts at: 3138 5385 1678 Size: 2247 2531 Fragments arranged by size: 2531 2247 701 491 107 70 41 25 18 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 4879 4911 4953 Size: 32 135 32 968 32 2307 32 42 Cuts at: 4953 4985 1373 Size: 32 2626 Fragments arranged by size: 2626 2307 968 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KasI G'GCGC_C Cuts at: 2511 2511 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 3837 3837 Size: 6238 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5352 5536 1305 Size: 4047 184 2007 Fragments arranged by size: 4047 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2858 10 Size: 54 1425 343 1026 3390 Fragments arranged by size: 3390 1425 1026 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2699 3248 3290 3296 4196 4555 4714 Size: 648 1533 549 42 6 900 359 159 Cuts at: 4714 518 Size: 2042 Fragments arranged by size: 2042 1533 900 648 549 359 159 42 6 MspA1I CmG'CkG Cuts at: 1606 2517 3388 3400 3472 3654 4331 5479 5724 Size: 911 871 12 72 182 677 1148 245 Cuts at: 5724 1606 Size: 2120 Fragments arranged by size: 2120 1148 911 871 677 245 182 72 12 NaeI GCC'GGC Cuts at: 2495 2495 Size: 6238 NarI GG'CG_CC Cuts at: 2512 2512 Size: 6238 NcoI C'CATG_G Cuts at: 513 1065 2004 3342 513 Size: 552 939 1338 3409 Fragments arranged by size: 3409 1338 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NgoMIV G'CCGG_C Cuts at: 2493 2493 Size: 6238 NheI G'CTAG_C Cuts at: 1052 3023 1052 Size: 1971 4267 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3223 3247 3468 3532 3596 5141 1132 Size: 97 1994 24 221 64 64 1545 2229 Fragments arranged by size: 2229 1994 1545 221 97 64 64 24 PasI CC'CwG_GG Cuts at: 1348 3205 1348 Size: 1857 4381 PciI A'CATG_T Cuts at: 3219 3464 5137 3219 Size: 245 1673 4320 Fragments arranged by size: 4320 1673 245 PflMI CCAn_nnn'nTGG Cuts at: 1108 2989 1108 Size: 1881 4357 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 3001 2548 Size: 453 5785 PshAI GACnn'nnGTC Cuts at: 1613 3465 1613 Size: 1852 4386 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3590 830 Size: 2760 3478 PvuI CG_AT'CG Cuts at: 2001 3537 2001 Size: 1536 4702 PvuII CAG'CTG Cuts at: 3400 3400 Size: 6238 SacII CC_GC'GG Cuts at: 3389 3389 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SapI GCTCTTCn'nnn_ Cuts at: 2546 2546 Size: 6238 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 3451 4486 6198 3451 Size: 1035 1712 3491 Fragments arranged by size: 3491 1712 1035 SfcI C'TryA_G Cuts at: 826 1045 3586 4721 5402 5593 826 Size: 219 2541 1135 681 191 1471 Fragments arranged by size: 2541 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2513 2513 Size: 6238 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4342 5243 5505 820 Size: 197 926 2399 901 262 1553 Fragments arranged by size: 2399 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3247 3596 1132 Size: 2115 349 3774 Fragments arranged by size: 3774 2115 349 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 2732 1517 Size: 1215 5023 StyI C'CwwG_G Cuts at: 513 1065 2004 2011 2904 2913 3063 3342 3677 Size: 552 939 7 893 9 150 279 335 Cuts at: 3677 513 Size: 3074 Fragments arranged by size: 3074 939 893 552 335 279 150 9 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2315 4265 4282 4435 4620 5065 325 Size: 1255 735 1950 17 153 185 445 1498 Fragments arranged by size: 1950 1498 1255 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3449 4484 6196 Size: 274 80 33 51 1443 1472 1035 1712 Cuts at: 6196 96 Size: 138 Fragments arranged by size: 1712 1472 1443 1035 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6198 6230 6198 Size: 32 6206 Tth111I GACn'n_nGTC Cuts at: 1316 3235 1316 Size: 1919 4319 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2626 2920 3202 2626 Size: 294 282 5662 Fragments arranged by size: 5662 294 282 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 3317 4367 3317 Size: 1050 5188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI