Restriction map for
pFN21AB4266
(Linear) (Six-base) MAPSORT of: KIBB4266.seq Check: 9986 from: 1 to: 1533
KIBB4266.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AclI AA'CG_TT
Cuts at: 0 677 1533
Size: 677 856
AfeI AGC'GCT
Cuts at: 0 640 1533
Size: 640 893
AflIII A'CryG_T
Cuts at: 0 242 1214 1459 1533
Size: 242 972 245 74
Fragments arranged by size:
972 245 242 74
AleI CACnn'nnGTG
Cuts at: 0 694 1533
Size: 694 839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 712 1533
Size: 712 821
ApoI r'AATT_y
Cuts at: 0 1481 1533
Size: 1481 52
AvaI C'yCGr_G
Cuts at: 0 423 1533
Size: 423 1110
BanI G'GyrC_C
Cuts at: 0 506 1533
Size: 506 1027
BanII G_rGCy'C
Cuts at: 0 119 308 1254 1533
Size: 119 189 946 279
Fragments arranged by size:
946 279 189 119
BbeI G_GCGC'C
Cuts at: 0 510 1533
Size: 510 1023
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 1533
Size: 180 1353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 1533
Size: 81 34 1418
Fragments arranged by size:
1418 81 34
BclI T'GATC_A
Cuts at: 0 32 1533
Size: 32 1501
BglI GCCn_nnn'nGGC
Cuts at: 0 109 182 349 494 1533
Size: 109 73 167 145 1039
Fragments arranged by size:
1039 167 145 109 73
BlpI GC'TnA_GC
Cuts at: 0 883 1343 1533
Size: 883 460 190
Fragments arranged by size:
883 460 190
Bme1580I G_kGCm'C
Cuts at: 0 1113 1298 1533
Size: 1113 185 235
Fragments arranged by size:
1113 235 185
BmgBI CAC'GTC
Cuts at: 0 243 300 915 1229 1533
Size: 243 57 615 314 304
Fragments arranged by size:
615 314 304 243 57
BmtI G_CTAG'C
Cuts at: 0 1022 1533
Size: 1022 511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 646 892 1533
Size: 466 180 246 641
Fragments arranged by size:
641 466 246 180
Bpu10I CC'TnA_GC
Cuts at: 0 1332 1533
Size: 1332 201
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 609 622 1533
Size: 286 323 13 911
Fragments arranged by size:
911 323 286 13
BsaHI Gr'CG_yC
Cuts at: 0 122 185 341 404 507 749 1533
Size: 122 63 156 63 103 242 784
Fragments arranged by size:
784 242 156 122 103 63 63
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 280 361 365 1533
Size: 277 3 81 4 1168
Fragments arranged by size:
1168 277 81 4 3
BseYI C'CCAG_C
Cuts at: 0 234 918 1126 1395 1533
Size: 234 684 208 269 138
Fragments arranged by size:
684 269 234 208 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1533
Size: 211 1322
BsiEI CG_ry'CG
Cuts at: 0 174 1533
Size: 174 1359
BsiHKAI G_wGCw'C
Cuts at: 0 1142 1277 1533
Size: 1142 135 256
Fragments arranged by size:
1142 256 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 712 1533
Size: 712 821
Bsp1286I G_dGCh'C
Cuts at: 0 119 308 1113 1142 1254 1277 1298 1533
Size: 119 189 805 29 112 23 21 235
Fragments arranged by size:
805 235 189 119 112 29 23 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1492 1533
Size: 1492 41
BsrBI CCG'CTC
Cuts at: 0 68 255 887 1533
Size: 68 187 632 646
Fragments arranged by size:
646 632 187 68
BsrDI GCAATG_nn'
Cuts at: 0 690 1533
Size: 690 843
BsrFI r'CCGG_y
Cuts at: 0 488 1533
Size: 488 1045
BssSI C'ACGA_G
Cuts at: 0 634 1533
Size: 634 899
BstEII G'GTnAC_C
Cuts at: 0 1185 1533
Size: 1185 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1533
Size: 462 1071
BstYI r'GATC_y
Cuts at: 0 1399 1533
Size: 1399 134
BtgI C'CryG_G
Cuts at: 0 1181 1337 1381 1533
Size: 1181 156 44 152
Fragments arranged by size:
1181 156 152 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1266 1533
Size: 1266 267
BtsI GCAGTG_nn'
Cuts at: 0 228 1533
Size: 228 1305
DraIII CAC_nnn'GTG
Cuts at: 0 984 1533
Size: 984 549
EaeI y'GGCC_r
Cuts at: 0 486 851 1384 1533
Size: 486 365 533 149
Fragments arranged by size:
533 486 365 149
EarI CTCTTCn'nnn_
Cuts at: 0 541 595 1533
Size: 541 54 938
Fragments arranged by size:
938 541 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 1533
Size: 127 1406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 646 892 1533
Size: 466 180 246 641
Fragments arranged by size:
641 466 246 180
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1517 1533
Size: 1517 16
EcoO109I rG'GnC_Cy
Cuts at: 0 543 996 1533
Size: 543 453 537
Fragments arranged by size:
543 537 453
FseI GG_CCGG'CC
Cuts at: 0 492 1533
Size: 492 1041
FspI TGC'GCA
Cuts at: 0 79 949 958 1533
Size: 79 870 9 575
Fragments arranged by size:
870 575 79 9
FspAI rTGC'GCAy
Cuts at: 0 949 1533
Size: 949 584
HaeII r_GCGC'y
Cuts at: 0 374 392 462 469 510 535 642 1133
Size: 374 18 70 7 41 25 107 491
Cuts at: 1133 1533
Size: 400
Fragments arranged by size:
491 400 374 107 70 41 25 18
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 1533
Size: 535 32 966
Fragments arranged by size:
966 535 32
KasI G'GCGC_C
Cuts at: 0 506 1533
Size: 506 1027
MscI TGG'CCA
Cuts at: 0 853 1533
Size: 853 680
MslI CAynn'nnrTG
Cuts at: 0 694 1243 1285 1291 1533
Size: 694 549 42 6 242
Fragments arranged by size:
694 549 242 42 6
MspA1I CmG'CkG
Cuts at: 0 512 1383 1395 1467 1533
Size: 512 871 12 72 66
Fragments arranged by size:
871 512 72 66 12
NaeI GCC'GGC
Cuts at: 0 490 1533
Size: 490 1043
NarI GG'CG_CC
Cuts at: 0 507 1533
Size: 507 1026
NcoI C'CATG_G
Cuts at: 0 1337 1533
Size: 1337 196
NgoMIV G'CCGG_C
Cuts at: 0 488 1533
Size: 488 1045
NheI G'CTAG_C
Cuts at: 0 1018 1533
Size: 1018 515
NspI r_CATG'y
Cuts at: 0 1218 1242 1463 1527 1533
Size: 1218 24 221 64 6
Fragments arranged by size:
1218 221 64 24 6
PasI CC'CwG_GG
Cuts at: 0 1200 1533
Size: 1200 333
PciI A'CATG_T
Cuts at: 0 1214 1459 1533
Size: 1214 245 74
Fragments arranged by size:
1214 245 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 984 1533
Size: 984 549
PpuMI rG'GwC_Cy
Cuts at: 0 543 996 1533
Size: 543 453 537
Fragments arranged by size:
543 537 453
PshAI GACnn'nnGTC
Cuts at: 0 1460 1533
Size: 1460 73
PvuII CAG'CTG
Cuts at: 0 1395 1533
Size: 1395 138
SacII CC_GC'GG
Cuts at: 0 1384 1533
Size: 1384 149
SapI GCTCTTCn'nnn_
Cuts at: 0 541 1533
Size: 541 992
ScaI AGT'ACT
Cuts at: 0 1446 1533
Size: 1446 87
SfoI GGC'GCC
Cuts at: 0 508 1533
Size: 508 1025
SphI G_CATG'C
Cuts at: 0 1242 1533
Size: 1242 291
StuI AGG'CCT
Cuts at: 0 727 1533
Size: 727 806
StyI C'CwwG_G
Cuts at: 0 6 899 908 1058 1337 1533
Size: 6 893 9 150 279 196
Fragments arranged by size:
893 279 196 150 9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 1533
Size: 310 1223
TatI w'GTAC_w
Cuts at: 0 1444 1533
Size: 1444 89
Tth111I GACn'n_nGTC
Cuts at: 0 1230 1533
Size: 1230 303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 915 1197 1533
Size: 621 294 282 336
Fragments arranged by size:
621 336 294 282
XmnI GAAnn'nnTTC
Cuts at: 0 1312 1533
Size: 1312 221
Enzymes that do cut:
AclI AfeI AflIII AleI AlfI ApoI AvaI BanI
BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I
BmgBI BmtI BpmI Bpu10I BsaI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FseI
FspI FspAI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PpuMI PshAI PvuII SacII SapI ScaI SfoI
SphI StuI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BglII BmrI BplI
BpuEI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SbfI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TstI
XbaI XhoI XmaI ZraI