Restriction map for
pFN21AB4255
##################################
# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbrPI BbvCI
BcgI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HgaI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MfeI MluI MseI MslI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 271 TCCGGA T^CCGGA
AciI 524 CCGC CCGC(-3/-1)
AcsI 10 GAATTT [GA]^AATT[CT]
AcyI 459 GGCGCC G[GA]^CG[CT]C
Alw44I 392 GTGCAC G^TGCAC
ApaI 324 GGGCCC GGGCC^C
ApaLI 392 GTGCAC G^TGCAC
ApoI 10 GAATTT [GA]^AATT[CT]
BamHI 436 GGATCC G^GATCC
BanII 324 GGGCCC G[GA]GC[CT]^C
BbeI 459 GGCGCC GGCGC^C
BbsI 444 GTCTTC GAAGAC(2/6)
BcnI 322 CCGGG CC^[GC]GG
BglI 544 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 444 GTCTTC GAAGAC(2/6)
BsaHI 459 GGCGCC G[GA]^CG[CT]C
BseAI 271 TCCGGA T^CCGGA
BspEI 271 TCCGGA T^CCGGA
BsrBI 524 CCGCTC CCGCTC(-3/-3)
BsrI 662 CCAGT ACTGG(1/-1)
Cac8I 98 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 460 GCGC GCG^C
DrdI 275 GACCATCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 88 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 523 CCCGC CCCGC(4/6)
HaeII 459 GGCGCC [GA]GCGC^[CT]
HhaI 460 GCGC GCG^C
Hin1I 459 GGCGCC G[GA]^CG[CT]C
HinP1I 460 GCGC G^CGC
KasI 459 GGCGCC G^GCGCC
MroI 271 TCCGGA T^CCGGA
NarI 459 GGCGCC GG^CGCC
NciI 322 CCGGG CC^[GC]GG
NdeI 386 CATATG CA^TATG
PstI 410 CTGCAG CTGCA^G
SexAI 291 ACCTGGT A^CC[AT]GGT
SfaNI 25 GATGC GCATC(5/9)
SfoI 459 GGCGCC GGC^GCC
SmlI 92 CTCAAG C^T[CT][GA]AG
Tsp509I 11 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 81 TGGCCA TGG^CCA
100 TGGCCA
BanI 459 GGCGCC G^G[CT][GA]CC
574 GGCACC
BpmI 242 CTCCAG CTGGAG(16/14)
509 CTCCAG
BsaWI 232 ACCGGA [AT]^CCGG[AT]
271 TCCGGA
BsmFI 597 GGGAC GGGAC(10/14)
284 GTCCC
BstXI 540 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
586 CCAGGAGCCTGG
BstYI 402 AGATCC [GA]^GATC[CT]
436 GGATCC
DraIII 161 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
290 CACCTGGTG
DsaI 430 CCATGG C^C[GA][CT]GG
568 CCATGG
EaeI 81 TGGCCA [CT]^GGCC[GA]
100 TGGCCA
HinfI 155 GACTC G^A[ACGT]TC
647 GACTC
MboII 139 TCTTC GAAGA(8/7)
445 TCTTC
MflI 402 AGATCC [GA]^GATC[CT]
436 GGATCC
MluNI 81 TGGCCA TGG^CCA
100 TGGCCA
MscI 81 TGGCCA TGG^CCA
100 TGGCCA
MspA1I 407 CAGCTG C[AC]G^C[GT]G
413 CAGCTG
MwoI 98 GCTGGCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
544 GCCCCTGGGGC
NcoI 430 CCATGG C^CATGG
568 CCATGG
PleI 155 GACTC GAGTC(4/5)
647 GACTC
PpuMI 246 AGGACCC [GA]G^G[AT]CC[CT]
687 AGGACCT
PvuII 407 CAGCTG CAG^CTG
413 CAGCTG
SfcI 410 CTGCAG C^T[GA][CT]AG
623 CTACAG
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
180 CACTG
XcmI 559 CCACCTGTTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
583 CCACCAGGAGCCTGG
XhoII 402 AGATCC [GA]^GATC[CT]
436 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI AspHI AvaII BbvI
BciVI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HapII HpaII
ItaI MboI MnlI MspI MvaI
NdeII NlaIII NlaIV PflMI Sau3AI
Sau96I ScrFI StyI TseI Van91I