(Circular) (Six-base) MAPSORT of: pFN21AB4255.seq Check: 302 from: 1 to: 5440 pFN21AB4255 5440 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2130 2673 3503 4887 5155 1632 Size: 498 543 830 1384 268 1917 Fragments arranged by size: 1917 1384 830 543 498 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 3183 4755 3183 Size: 1572 3868 ApaI G_GGCC'C Cuts at: 2333 2333 Size: 5440 ApaLI G'TGCA_C Cuts at: 2397 3497 4653 2397 Size: 1100 1156 3184 Fragments arranged by size: 3184 1156 1100 ApoI r'AATT_y Cuts at: 1493 2015 2749 2975 3127 5236 1493 Size: 522 734 226 152 2109 1697 Fragments arranged by size: 2109 1697 734 522 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2765 1870 Size: 73 822 4545 Fragments arranged by size: 4545 822 73 BamHI G'GATC_C Cuts at: 2441 2770 3137 2441 Size: 329 367 4744 Fragments arranged by size: 4744 367 329 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 2579 2761 4216 618 Size: 325 217 627 677 115 182 1455 1842 Fragments arranged by size: 1842 1455 677 627 325 217 182 115 BanII G_rGCy'C Cuts at: 1391 2333 2759 1391 Size: 942 426 4072 Fragments arranged by size: 4072 942 426 BbeI G_GCGC'C Cuts at: 2468 2468 Size: 5440 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2442 928 Size: 971 543 3926 Fragments arranged by size: 3926 971 543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2229 2496 2649 3332 4542 2229 Size: 267 153 683 1210 3127 Fragments arranged by size: 3127 1210 683 267 153 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2555 4051 136 Size: 107 122 71 631 605 883 1496 1525 Fragments arranged by size: 1525 1496 883 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 2333 2401 2582 3501 4657 1790 Size: 543 68 181 919 1156 2573 Fragments arranged by size: 2573 1156 919 543 181 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2661 3171 4129 Size: 416 53 83 399 24 1244 510 958 Cuts at: 4129 442 Size: 1753 Fragments arranged by size: 1753 1244 958 510 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2499 4100 1239 Size: 993 267 1601 2579 Fragments arranged by size: 2579 1601 993 267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2082 3565 4430 4728 2082 Size: 1483 865 298 2794 Fragments arranged by size: 2794 1483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2465 3247 3629 4264 Size: 53 83 186 936 932 782 382 635 Cuts at: 4264 275 Size: 1451 Fragments arranged by size: 1451 936 932 782 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2237 2276 3873 4271 4545 4692 5103 2237 Size: 39 1597 398 274 147 411 2574 Fragments arranged by size: 2574 1597 411 398 274 147 39 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5215 Fragments arranged by size: 5215 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 2523 2526 2529 1186 Size: 899 438 3 3 4097 Fragments arranged by size: 4097 899 438 3 3 BseYI C'CCAG_C Cuts at: 2257 4643 5139 2257 Size: 2386 496 2558 Fragments arranged by size: 2558 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4867 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2890 3651 4679 1540 Size: 275 186 889 761 1028 2301 Fragments arranged by size: 2301 1028 889 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2140 2401 2707 3501 3586 4657 2140 Size: 261 306 794 85 1071 2923 Fragments arranged by size: 2923 1071 794 306 261 85 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2140 2333 2401 2582 2707 2759 3501 Size: 399 350 193 68 181 125 52 742 Cuts at: 3501 3586 4657 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 193 181 125 85 68 52 BspEI T'CCGG_A Cuts at: 2276 5103 2276 Size: 2827 2613 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2795 844 Size: 1951 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2531 3327 1394 Size: 429 708 796 3507 Fragments arranged by size: 3507 796 708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3935 4109 65 Size: 1183 2687 174 1396 Fragments arranged by size: 2687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2552 2598 2552 Size: 46 5394 BstYI r'GATC_y Cuts at: 1972 2407 2441 2770 3137 3522 3539 4991 5077 Size: 435 34 329 367 385 17 1452 86 Cuts at: 5077 5089 5435 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 435 385 367 346 329 86 34 17 12 BtgI C'CryG_G Cuts at: 513 1065 2435 2573 513 Size: 552 1370 138 3380 Fragments arranged by size: 3380 1370 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3750 3770 2956 Size: 794 20 4626 Fragments arranged by size: 4626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DraIII CAC_nnn'GTG Cuts at: 2171 2300 2171 Size: 129 5311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2286 4447 809 Size: 507 970 2161 1802 Fragments arranged by size: 2161 1802 970 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2105 2887 Size: 54 1425 50 275 18 256 19 782 Cuts at: 2887 3776 5405 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 782 275 256 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3370 1353 Size: 2017 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4027 4401 4547 387 Size: 1064 138 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2232 2499 2673 3503 4100 4887 Size: 393 498 102 267 174 830 597 787 Cuts at: 4887 5155 1239 Size: 268 1524 Fragments arranged by size: 1524 830 787 597 498 393 268 267 174 102 EcoNI CCTnn'n_nnAGG Cuts at: 2097 2097 Size: 5440 EcoO109I rG'GnC_Cy Cuts at: 2252 2330 2693 2252 Size: 78 363 4999 Fragments arranged by size: 4999 363 78 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 2468 4587 1678 Size: 790 2119 2531 Fragments arranged by size: 2531 2119 790 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373 Size: 32 135 32 2509 32 42 32 2626 Fragments arranged by size: 2626 2509 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KasI G'GCGC_C Cuts at: 2464 2464 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 4554 4738 1305 Size: 834 2415 184 2007 Fragments arranged by size: 2415 2007 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2088 2107 10 Size: 54 1425 343 256 19 3343 Fragments arranged by size: 3343 1425 343 256 54 19 MslI CAynn'nnrTG Cuts at: 518 1166 3398 3757 3916 518 Size: 648 2232 359 159 2042 Fragments arranged by size: 2232 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2414 2420 2856 3533 4681 4926 1606 Size: 808 6 436 677 1148 245 2120 Fragments arranged by size: 2120 1148 808 677 436 245 6 NarI GG'CG_CC Cuts at: 2465 2465 Size: 5440 NcoI C'CATG_G Cuts at: 513 1065 2435 2573 513 Size: 552 1370 138 3380 Fragments arranged by size: 3380 1370 552 138 NdeI CA'TA_TG Cuts at: 387 2392 387 Size: 2005 3435 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2798 4343 1132 Size: 97 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 97 PasI CC'CwG_GG Cuts at: 1348 2552 1348 Size: 1204 4236 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 2121 2300 2717 1108 Size: 1013 179 417 3831 Fragments arranged by size: 3831 1013 417 179 PfoI T'CCnGG_A Cuts at: 2248 2248 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2252 2693 2252 Size: 441 4999 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PspOMI G'GGCC_C Cuts at: 2329 2329 Size: 5440 PstI C_TGCA'G Cuts at: 830 2419 2792 830 Size: 1589 373 3478 Fragments arranged by size: 3478 1589 373 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 PvuII CAG'CTG Cuts at: 2414 2420 2414 Size: 6 5434 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SexAI A'CCwGG_T Cuts at: 2296 2296 Size: 5440 SfcI C'TryA_G Cuts at: 826 1045 2415 2628 2788 3923 4604 4795 826 Size: 219 1370 213 160 1135 681 191 1471 Fragments arranged by size: 1471 1370 1135 681 219 213 191 160 SfoI GGC'GCC Cuts at: 2466 2466 Size: 5440 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2097 3544 4445 4707 820 Size: 197 926 154 1447 901 262 1553 Fragments arranged by size: 1553 1447 926 901 262 197 154 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2120 2435 2459 2573 2879 513 Size: 552 1055 315 24 114 306 3074 Fragments arranged by size: 3074 1055 552 315 306 114 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2416 3467 3484 3637 3822 4267 325 Size: 1255 836 1051 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1051 836 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5400 5432 5400 Size: 32 5408 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2571 2595 2571 Size: 24 5416 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI