Restriction map for
pFN21AB4255
(Linear) (Six-base) MAPSORT of: KIBB4255.seq Check: 1726 from: 1 to: 735
KIBB4255.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 668 735
Size: 125 543 67
Fragments arranged by size:
543 125 67
ApaI G_GGCC'C
Cuts at: 0 328 735
Size: 328 407
ApaLI G'TGCA_C
Cuts at: 0 392 735
Size: 392 343
ApoI r'AATT_y
Cuts at: 0 10 735
Size: 10 725
BamHI G'GATC_C
Cuts at: 0 436 735
Size: 436 299
BanI G'GyrC_C
Cuts at: 0 459 574 735
Size: 459 115 161
Fragments arranged by size:
459 161 115
BanII G_rGCy'C
Cuts at: 0 328 735
Size: 328 407
BbeI G_GCGC'C
Cuts at: 0 463 735
Size: 463 272
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 735
Size: 437 298
BciVI GTATCCnnnnn_n'
Cuts at: 0 224 491 644 735
Size: 224 267 153 91
Fragments arranged by size:
267 224 153 91
BglI GCCn_nnn'nGGC
Cuts at: 0 550 735
Size: 550 185
Bme1580I G_kGCm'C
Cuts at: 0 328 396 577 735
Size: 328 68 181 158
Fragments arranged by size:
328 181 158 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 656 735
Size: 656 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 494 735
Size: 227 267 241
Fragments arranged by size:
267 241 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 735
Size: 77 658
BsaHI Gr'CG_yC
Cuts at: 0 460 735
Size: 460 275
BsaWI w'CCGG_w
Cuts at: 0 232 271 735
Size: 232 39 464
Fragments arranged by size:
464 232 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 518 521 524 735
Size: 80 438 3 3 211
Fragments arranged by size:
438 211 80 3 3
BseYI C'CCAG_C
Cuts at: 0 252 735
Size: 252 483
BsiHKAI G_wGCw'C
Cuts at: 0 135 396 702 735
Size: 135 261 306 33
Fragments arranged by size:
306 261 135 33
Bsp1286I G_dGCh'C
Cuts at: 0 135 328 396 577 702 735
Size: 135 193 68 181 125 33
Fragments arranged by size:
193 181 135 125 68 33
BspEI T'CCGG_A
Cuts at: 0 271 735
Size: 271 464
BsrBI CCG'CTC
Cuts at: 0 526 735
Size: 526 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 547 593 735
Size: 547 46 142
Fragments arranged by size:
547 142 46
BstYI r'GATC_y
Cuts at: 0 402 436 735
Size: 402 34 299
Fragments arranged by size:
402 299 34
BtgI C'CryG_G
Cuts at: 0 430 568 735
Size: 430 138 167
Fragments arranged by size:
430 167 138
DraIII CAC_nnn'GTG
Cuts at: 0 166 295 735
Size: 166 129 440
Fragments arranged by size:
440 166 129
DrdI GACnn_nn'nnGTC
Cuts at: 0 281 735
Size: 281 454
EaeI y'GGCC_r
Cuts at: 0 81 100 735
Size: 81 19 635
Fragments arranged by size:
635 81 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 227 494 668 735
Size: 125 102 267 174 67
Fragments arranged by size:
267 174 125 102 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 92 735
Size: 92 643
EcoO109I rG'GnC_Cy
Cuts at: 0 247 325 688 735
Size: 247 78 363 47
Fragments arranged by size:
363 247 78 47
HaeII r_GCGC'y
Cuts at: 0 463 735
Size: 463 272
KasI G'GCGC_C
Cuts at: 0 459 735
Size: 459 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 735
Size: 134 601
MscI TGG'CCA
Cuts at: 0 83 102 735
Size: 83 19 633
Fragments arranged by size:
633 83 19
MspA1I CmG'CkG
Cuts at: 0 409 415 735
Size: 409 6 320
Fragments arranged by size:
409 320 6
NarI GG'CG_CC
Cuts at: 0 460 735
Size: 460 275
NcoI C'CATG_G
Cuts at: 0 430 568 735
Size: 430 138 167
Fragments arranged by size:
430 167 138
NdeI CA'TA_TG
Cuts at: 0 387 735
Size: 387 348
PasI CC'CwG_GG
Cuts at: 0 547 735
Size: 547 188
PflMI CCAn_nnn'nTGG
Cuts at: 0 116 295 712 735
Size: 116 179 417 23
Fragments arranged by size:
417 179 116 23
PfoI T'CCnGG_A
Cuts at: 0 243 735
Size: 243 492
PpuMI rG'GwC_Cy
Cuts at: 0 247 688 735
Size: 247 441 47
Fragments arranged by size:
441 247 47
PspOMI G'GGCC_C
Cuts at: 0 324 735
Size: 324 411
PstI C_TGCA'G
Cuts at: 0 414 735
Size: 414 321
PvuII CAG'CTG
Cuts at: 0 409 415 735
Size: 409 6 320
Fragments arranged by size:
409 320 6
SexAI A'CCwGG_T
Cuts at: 0 291 735
Size: 291 444
SfcI C'TryA_G
Cuts at: 0 410 623 735
Size: 410 213 112
Fragments arranged by size:
410 213 112
SfoI GGC'GCC
Cuts at: 0 461 735
Size: 461 274
SmlI C'TyrA_G
Cuts at: 0 92 735
Size: 92 643
StyI C'CwwG_G
Cuts at: 0 115 430 454 568 735
Size: 115 315 24 114 167
Fragments arranged by size:
315 167 115 114 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 411 735
Size: 411 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 566 590 735
Size: 566 24 145
Fragments arranged by size:
566 145 24
Enzymes that do cut:
AcuI ApaI ApaLI ApoI BamHI BanI BanII BbeI
BbsI BciVI BglI Bme1580I BmrI BpmI BpuEI BsaHI
BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BsrBI BstXI
BstYI BtgI DraIII DrdI EaeI Eco57MI EcoNI EcoO109I
HaeII KasI MmeI MscI MspA1I NarI NcoI NdeI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SexAI
SfcI SfoI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI AscI AseI
AsiSI AvaI AvrII BaeI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MslI NaeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI