(Circular) (Six-base) MAPSORT of: pFN21AB4240.seq Check: 6308 from: 1 to: 6118 pFN21AB4240 6118 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2434 2453 2434 Size: 19 6099 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 3461 1937 Size: 792 732 4594 Fragments arranged by size: 4594 792 732 Acc65I G'GTAC_C Cuts at: 2702 3439 2702 Size: 737 5381 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2406 2634 2966 3089 3211 4181 5565 5833 Size: 774 228 332 123 122 970 1384 268 Cuts at: 5833 1632 Size: 1917 Fragments arranged by size: 1917 1384 970 774 332 268 228 123 122 AfeI AGC'GCT Cuts at: 2549 2549 Size: 6118 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 3100 5017 5994 1203 Size: 1897 1917 977 1327 Fragments arranged by size: 1917 1897 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3365 1184 Size: 2181 3937 AlwNI CAG_nnn'CTG Cuts at: 3861 5433 3861 Size: 1572 4546 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2617 3427 3653 3805 5914 1493 Size: 1124 810 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 810 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2171 2198 2702 2747 Size: 325 217 627 248 136 27 504 45 Cuts at: 2747 2848 3439 4894 618 Size: 101 591 1455 1842 Fragments arranged by size: 1842 1455 627 591 504 325 248 217 136 101 45 27 BanII G_rGCy'C Cuts at: 1391 2540 3437 1391 Size: 1149 897 4072 Fragments arranged by size: 4072 1149 897 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5147 BbvCI CC'TCA_GC Cuts at: 2050 2245 2304 3363 3381 2050 Size: 195 59 1059 18 4787 Fragments arranged by size: 4787 1059 195 59 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3377 4729 136 Size: 107 122 71 631 605 1705 1352 1525 Fragments arranged by size: 1705 1525 1352 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3405 3535 3405 Size: 130 5988 Bme1580I G_kGCm'C Cuts at: 1790 2201 4179 5335 1790 Size: 411 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 411 BmgBI CAC'GTC Cuts at: 1318 3101 1318 Size: 1783 4335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 3849 4807 Size: 416 53 83 399 24 1064 1368 958 Cuts at: 4807 442 Size: 1753 Fragments arranged by size: 1753 1368 1064 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3207 4778 1239 Size: 1968 1571 2579 Fragments arranged by size: 2579 1968 1571 Bpu10I CC'TnA_GC Cuts at: 2050 2245 2304 3038 3363 3381 2050 Size: 195 59 734 325 18 4787 Fragments arranged by size: 4787 734 325 195 59 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4243 5108 5406 4243 Size: 865 298 4955 Fragments arranged by size: 4955 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2879 3909 493 Size: 711 1675 1030 2702 Fragments arranged by size: 2702 1675 1030 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2574 2874 3319 3351 1186 Size: 1096 292 300 445 32 3953 Fragments arranged by size: 3953 1096 445 300 292 32 BseYI C'CCAG_C Cuts at: 5321 5817 5321 Size: 496 5622 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 3381 1163 Size: 573 322 1323 3900 Fragments arranged by size: 3900 1323 573 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4179 4264 5335 4179 Size: 85 1071 4962 Fragments arranged by size: 4962 1071 85 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 2540 3437 4179 4264 5335 1391 Size: 399 411 339 897 742 85 1071 2174 Fragments arranged by size: 2174 1071 897 742 411 399 339 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2434 2453 2998 3357 3473 844 Size: 1590 19 545 359 116 3489 Fragments arranged by size: 3489 1590 545 359 116 19 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3328 4613 4787 65 Size: 1183 2080 1285 174 1396 Fragments arranged by size: 2080 1396 1285 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3290 3448 3815 4200 4217 5669 5755 5767 Size: 1318 158 367 385 17 1452 86 12 Cuts at: 5767 6113 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1318 385 367 346 158 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3256 513 Size: 552 939 1252 3375 Fragments arranged by size: 3375 1252 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2298 2933 3390 3634 4428 4448 2298 Size: 635 457 244 794 20 3968 Fragments arranged by size: 3968 794 635 457 244 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 2497 3422 2497 Size: 925 5193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2668 5125 809 Size: 507 1352 2457 1802 Fragments arranged by size: 2457 1802 1352 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3565 4454 6083 Size: 54 1425 50 275 18 1735 889 1629 Cuts at: 6083 8 Size: 43 Fragments arranged by size: 1735 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 2557 2650 2690 4048 1353 Size: 807 397 93 40 1358 3423 Fragments arranged by size: 3423 1358 807 397 93 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2124 2674 3068 3111 4705 5079 Size: 1064 138 535 550 394 43 1594 374 Cuts at: 5079 5225 387 Size: 146 1280 Fragments arranged by size: 1594 1280 1064 550 535 394 374 146 138 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2406 2634 2966 3089 3207 3211 4181 Size: 393 774 228 332 123 118 4 970 Cuts at: 4181 4778 5565 5833 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 970 787 774 597 393 332 268 228 123 118 4 EcoNI CCTnn'n_nnAGG Cuts at: 2589 2589 Size: 6118 EcoO109I rG'GnC_Cy Cuts at: 2808 2808 Size: 6118 EcoRI G'AATT_C Cuts at: 2617 3427 3805 2617 Size: 810 378 4930 Fragments arranged by size: 4930 810 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2781 3241 3430 2781 Size: 460 189 5469 Fragments arranged by size: 5469 460 189 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 2139 2551 5265 1678 Size: 461 412 2714 2531 Fragments arranged by size: 2714 2531 461 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 2174 2206 2258 Size: 32 135 32 514 32 56 32 52 Cuts at: 2258 2290 2353 2385 2501 2533 4759 4791 4833 Size: 32 63 32 116 32 2226 32 42 Cuts at: 4833 4865 1373 Size: 32 2626 Fragments arranged by size: 2626 2226 514 135 116 63 56 52 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 2706 3443 2706 Size: 737 5381 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2846 3055 4076 4435 4594 518 Size: 648 1680 209 1021 359 159 2042 Fragments arranged by size: 2042 1680 1021 648 359 209 159 MspA1I CmG'CkG Cuts at: 1606 2249 2384 3534 4211 5359 5604 1606 Size: 643 135 1150 677 1148 245 2120 Fragments arranged by size: 2120 1150 1148 677 643 245 135 NcoI C'CATG_G Cuts at: 513 1065 2004 3256 513 Size: 552 939 1252 3375 Fragments arranged by size: 3375 1252 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3239 3476 5021 1132 Size: 97 2010 237 1545 2229 Fragments arranged by size: 2229 2010 1545 237 97 PasI CC'CwG_GG Cuts at: 1348 2723 1348 Size: 1375 4743 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PfoI T'CCnGG_A Cuts at: 2785 2785 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2808 2808 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PstI C_TGCA'G Cuts at: 830 2368 3470 830 Size: 1538 1102 3478 Fragments arranged by size: 3478 1538 1102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2249 2384 2249 Size: 135 5983 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SapI GCTCTTCn'nnn_ Cuts at: 2557 2557 Size: 6118 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SexAI A'CCwGG_T Cuts at: 2666 2666 Size: 6118 SfcI C'TryA_G Cuts at: 826 1045 2310 2364 3466 4601 5282 5473 826 Size: 219 1265 54 1102 1135 681 191 1471 Fragments arranged by size: 1471 1265 1135 1102 681 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4222 5123 5385 820 Size: 197 926 2279 901 262 1553 Fragments arranged by size: 2279 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2879 3909 493 Size: 2386 1030 2702 Fragments arranged by size: 2702 2386 1030 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3239 3476 1132 Size: 2107 237 3774 Fragments arranged by size: 3774 2107 237 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2004 3256 3557 513 Size: 552 939 1252 301 3074 Fragments arranged by size: 3074 1252 939 552 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2728 4145 4162 4315 4500 4945 325 Size: 1255 1148 1417 17 153 185 445 1498 Fragments arranged by size: 1498 1417 1255 1148 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4364 6076 96 Size: 274 80 33 51 1443 2387 1712 138 Fragments arranged by size: 2387 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6078 6110 6078 Size: 32 6086 Tth111I GACn'n_nGTC Cuts at: 1316 2513 1316 Size: 1197 4921 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3089 3152 3089 Size: 63 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 2621 4247 2621 Size: 1626 4492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI