(Circular) (Six-base) MAPSORT of: pFN21AB4220.seq Check: 2471 from: 1 to: 5746 pFN21AB4220 5746 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2623 3089 1937 Size: 686 466 4594 Fragments arranged by size: 4594 686 466 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2250 2357 3809 5193 5461 1632 Size: 608 10 107 1452 1384 268 1917 Fragments arranged by size: 1917 1452 1384 608 268 107 10 AflII C'TTAA_G Cuts at: 820 1017 2114 820 Size: 197 1097 4452 Fragments arranged by size: 4452 1097 197 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2940 1184 Size: 1756 3990 AlwNI CAG_nnn'CTG Cuts at: 2578 3489 5061 2578 Size: 911 1572 3263 Fragments arranged by size: 3263 1572 911 ApaI G_GGCC'C Cuts at: 2908 2908 Size: 5746 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2862 3071 1870 Size: 73 919 209 4545 Fragments arranged by size: 4545 919 209 73 BamHI G'GATC_C Cuts at: 2897 3076 3443 2897 Size: 179 367 5200 Fragments arranged by size: 5200 367 179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2670 3067 4522 618 Size: 325 217 627 883 397 1455 1842 Fragments arranged by size: 1842 1455 883 627 397 325 217 BanII G_rGCy'C Cuts at: 1391 2843 2908 3065 1391 Size: 1452 65 157 4072 Fragments arranged by size: 4072 1452 157 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2839 928 Size: 971 940 3835 Fragments arranged by size: 3835 971 940 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2908 3807 4963 1790 Size: 1118 899 1156 2573 Fragments arranged by size: 2573 1156 1118 899 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2641 2743 3477 Size: 416 53 83 399 24 1224 102 734 Cuts at: 3477 4435 442 Size: 958 1753 Fragments arranged by size: 1753 1224 958 734 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2857 2857 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2933 4406 1239 Size: 1694 1473 2579 Fragments arranged by size: 2579 1694 1473 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 4409 882 Size: 617 792 2118 2219 Fragments arranged by size: 2219 2118 792 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 2997 5740 2997 Size: 2743 3003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2433 4179 4577 4851 4998 5409 2433 Size: 1746 398 274 147 411 2770 Fragments arranged by size: 2770 1746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2548 2578 1178 Size: 30 165 30 1145 30 4346 Fragments arranged by size: 4346 1145 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3029 3032 3041 1186 Size: 1843 3 9 3891 Fragments arranged by size: 3891 1843 9 3 BseYI C'CCAG_C Cuts at: 2647 2907 4949 5445 2647 Size: 260 2042 496 2948 Fragments arranged by size: 2948 2042 496 260 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2632 1163 Size: 573 896 4277 Fragments arranged by size: 4277 896 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2527 2843 2919 3807 3892 4963 2527 Size: 316 76 888 85 1071 3310 Fragments arranged by size: 3310 1071 888 316 85 76 BsmI GAATG_Cn' Cuts at: 2591 3257 3350 2591 Size: 666 93 4987 Fragments arranged by size: 4987 666 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2527 2843 2908 2919 3065 3807 3892 Size: 399 737 316 65 11 146 742 85 Cuts at: 3892 4963 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 737 399 316 146 85 65 11 BspEI T'CCGG_A Cuts at: 2433 5409 2433 Size: 2976 2770 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 844 Size: 2257 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3042 4241 4415 65 Size: 1183 1794 1199 174 1396 Fragments arranged by size: 1794 1396 1199 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2783 1184 Size: 1599 4147 BstXI CCAn_nnnn'nTGG Cuts at: 2087 2408 2901 2087 Size: 321 493 4932 Fragments arranged by size: 4932 493 321 BstYI r'GATC_y Cuts at: 1972 2897 3076 3443 3828 3845 5297 5383 5395 Size: 925 179 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 925 385 367 346 179 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2214 2214 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2014 2049 529 Size: 35 1450 35 4226 Fragments arranged by size: 4226 1450 35 35 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DraIII CAC_nnn'GTG Cuts at: 2198 2198 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2424 4753 809 Size: 507 1108 2329 1802 Fragments arranged by size: 2329 1802 1108 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2707 3193 4082 Size: 54 1425 50 275 18 877 486 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 877 486 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3018 3676 1353 Size: 1665 658 3423 Fragments arranged by size: 3423 1665 658 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 2841 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2250 2357 2933 3809 4406 5193 Size: 393 608 10 107 576 876 597 787 Cuts at: 5193 5461 1239 Size: 268 1524 Fragments arranged by size: 1524 876 787 608 597 576 393 268 107 10 EcoNI CCTnn'n_nnAGG Cuts at: 2412 2882 2412 Size: 470 5276 EcoO109I rG'GnC_Cy Cuts at: 2092 2218 2234 2483 2092 Size: 126 16 249 5355 Fragments arranged by size: 5355 249 126 16 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2845 2877 2882 2914 2963 Size: 32 135 32 1273 32 5 32 49 Cuts at: 2963 2995 4387 4419 4461 4493 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 1273 135 49 42 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 2207 748 Size: 1459 4287 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4860 5044 1305 Size: 3555 184 2007 Fragments arranged by size: 3555 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2709 10 Size: 54 1425 343 877 3047 Fragments arranged by size: 3047 1425 877 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2828 2922 3704 4063 4222 518 Size: 648 1662 94 782 359 159 2042 Fragments arranged by size: 2042 1662 782 648 359 159 94 MspA1I CmG'CkG Cuts at: 1606 2448 2911 3162 3839 4987 5232 1606 Size: 842 463 251 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 463 251 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 2087 1348 Size: 739 5007 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 2368 2578 1108 Size: 1260 210 4276 Fragments arranged by size: 4276 1260 210 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2092 Size: 5746 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspOMI G'GGCC_C Cuts at: 2904 2904 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2448 2911 2448 Size: 463 5283 SacI G_AGCT'C Cuts at: 2843 2843 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SanDI GG'GwC_CC Cuts at: 2092 2092 Size: 5746 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2145 3094 4229 4910 5101 826 Size: 219 1100 949 1135 681 191 1471 Fragments arranged by size: 1471 1135 1100 949 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2114 3850 4751 5013 820 Size: 197 926 171 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 171 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2479 3185 513 Size: 552 1414 706 3074 Fragments arranged by size: 3074 1414 706 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2532 2725 3773 3790 3943 4128 4573 Size: 1255 952 193 1048 17 153 185 445 Cuts at: 4573 325 Size: 1498 Fragments arranged by size: 1498 1255 1048 952 445 193 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2180 3992 5704 Size: 274 80 33 51 1443 203 1812 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1812 1712 1443 274 203 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2517 2548 2549 2580 5706 5738 2517 Size: 31 1 31 3126 32 2525 Fragments arranged by size: 3126 2525 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI