(Circular) (Six-base) MAPSORT of: pFN21AB4166.seq Check: 7141 from: 1 to: 5887 pFN21AB4166 5887 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2028 3230 1937 Size: 91 1202 4594 Fragments arranged by size: 4594 1202 91 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3950 5334 5602 1632 Size: 2318 1384 268 1917 Fragments arranged by size: 2318 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 2334 4786 5763 1203 Size: 1131 2452 977 1327 Fragments arranged by size: 2452 1327 1131 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2781 1184 Size: 1597 4290 AlwNI CAG_nnn'CTG Cuts at: 2993 3630 5202 2993 Size: 637 1572 3678 Fragments arranged by size: 3678 1572 637 ApaI G_GGCC'C Cuts at: 3115 3165 3115 Size: 50 5837 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 3196 3422 3574 5683 1493 Size: 1703 226 152 2109 1697 Fragments arranged by size: 2109 1703 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3136 3212 1870 Size: 73 67 1126 76 4545 Fragments arranged by size: 4545 1126 76 73 67 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2429 3020 3050 3080 3208 Size: 325 217 627 642 591 30 30 128 Cuts at: 3208 4663 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 642 627 591 325 217 128 30 30 BanII G_rGCy'C Cuts at: 1391 3115 3165 3206 1391 Size: 1724 50 41 4072 Fragments arranged by size: 4072 1724 50 41 BbeI G_GCGC'C Cuts at: 2433 2433 Size: 5887 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BbvCI CC'TCA_GC Cuts at: 2037 2910 2037 Size: 873 5014 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2936 3779 4989 2936 Size: 843 1210 3834 Fragments arranged by size: 3834 1210 843 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2541 2886 5882 2541 Size: 345 2996 2546 Fragments arranged by size: 2996 2546 345 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2765 3053 3083 3115 3165 3948 5104 1790 Size: 975 288 30 32 50 783 1156 2573 Fragments arranged by size: 2573 1156 975 783 288 50 32 30 BmgBI CAC'GTC Cuts at: 1318 2140 1318 Size: 822 5065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2536 3618 4576 Size: 416 53 83 399 24 1119 1082 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1119 1082 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2961 1056 Size: 1905 3982 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2839 2839 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2394 2735 4547 1239 Size: 1155 341 1812 2579 Fragments arranged by size: 2579 1812 1155 341 Bpu10I CC'TnA_GC Cuts at: 2037 2910 2037 Size: 873 5014 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2840 4012 4877 5175 2840 Size: 1172 865 298 3552 Fragments arranged by size: 3552 1172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2060 3184 4550 882 Size: 617 561 1124 1366 2219 Fragments arranged by size: 2219 1366 1124 617 561 BsaAI yAC'GTr Cuts at: 493 1204 2284 3678 493 Size: 711 1080 1394 2702 Fragments arranged by size: 2702 1394 1080 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2289 2430 3694 4076 Size: 53 83 186 936 756 141 1264 382 Cuts at: 4076 4711 275 Size: 635 1451 Fragments arranged by size: 1451 1264 936 756 635 382 186 141 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5662 Fragments arranged by size: 5662 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2843 1186 Size: 1657 4230 BseYI C'CCAG_C Cuts at: 2262 2892 3146 5090 5586 2262 Size: 630 254 1944 496 2563 Fragments arranged by size: 2563 1944 630 496 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2660 2951 1163 Size: 573 924 291 4099 Fragments arranged by size: 4099 924 573 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2765 3053 3083 3115 3165 3206 3948 Size: 399 975 288 30 32 50 41 742 Cuts at: 3948 4033 5104 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 975 742 399 288 85 50 41 32 30 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 2682 3242 844 Size: 1381 457 560 3489 Fragments arranged by size: 3489 1381 560 457 BsrBI CCG'CTC Cuts at: 1394 1823 3033 3063 3093 3774 1394 Size: 429 1210 30 30 681 3507 Fragments arranged by size: 3507 1210 681 429 30 30 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 2169 2703 2896 4531 4718 1700 Size: 110 265 94 534 193 1635 187 2869 Fragments arranged by size: 2869 1635 534 265 193 187 110 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 2804 794 Size: 2010 3877 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstXI CCAn_nnnn'nTGG Cuts at: 2591 3172 2591 Size: 581 5306 BstYI r'GATC_y Cuts at: 1972 2541 2886 3217 3584 3969 3986 5438 5524 Size: 569 345 331 367 385 17 1452 86 Cuts at: 5524 5536 5882 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 569 385 367 346 345 331 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2346 2808 3171 513 Size: 552 1281 462 363 3229 Fragments arranged by size: 3229 1281 552 462 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2765 2805 3403 4197 4217 2765 Size: 40 598 794 20 4435 Fragments arranged by size: 4435 794 598 40 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DraIII CAC_nnn'GTG Cuts at: 2148 2650 2148 Size: 502 5385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2296 2898 3334 Size: 54 1425 50 275 18 466 602 436 Cuts at: 3334 4223 5852 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 602 466 436 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 3817 1353 Size: 884 1580 3423 Fragments arranged by size: 3423 1580 884 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2879 4474 4848 4994 387 Size: 1064 138 1290 1595 374 146 1280 Fragments arranged by size: 1595 1290 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2394 2735 3950 4547 5334 5602 1239 Size: 393 762 341 1215 597 787 268 1524 Fragments arranged by size: 1524 1215 787 762 597 393 341 268 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2036 Size: 5887 EcoO109I rG'GnC_Cy Cuts at: 2479 3111 2479 Size: 632 5255 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2433 3159 5034 1678 Size: 755 726 1875 2531 Fragments arranged by size: 2531 1875 755 726 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2545 2577 4528 4560 4602 Size: 32 135 32 973 32 1951 32 42 Cuts at: 4602 4634 1373 Size: 32 2626 Fragments arranged by size: 2626 1951 973 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KasI G'GCGC_C Cuts at: 2429 2429 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2298 10 Size: 54 1425 343 466 3599 Fragments arranged by size: 3599 1425 466 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2576 3845 4204 4363 518 Size: 648 1035 375 1269 359 159 2042 Fragments arranged by size: 2042 1269 1035 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2356 3303 3980 5128 5373 1606 Size: 750 947 677 1148 245 2120 Fragments arranged by size: 2120 1148 947 750 677 245 NaeI GCC'GGC Cuts at: 2705 2898 2705 Size: 193 5694 NarI GG'CG_CC Cuts at: 2430 2430 Size: 5887 NcoI C'CATG_G Cuts at: 513 1065 2346 3171 513 Size: 552 1281 825 3229 Fragments arranged by size: 3229 1281 825 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NgoMIV G'CCGG_C Cuts at: 2703 2896 2703 Size: 193 5694 NheI G'CTAG_C Cuts at: 1052 2957 1052 Size: 1905 3982 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2251 2338 2950 3245 4790 1132 Size: 97 1022 87 612 295 1545 2229 Fragments arranged by size: 2229 1545 1022 612 295 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 2334 4786 2334 Size: 2452 3435 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PfoI T'CCnGG_A Cuts at: 2100 2371 2100 Size: 271 5616 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PmlI CAC'GTG Cuts at: 2284 2284 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PspOMI G'GGCC_C Cuts at: 3111 3161 3111 Size: 50 5837 PstI C_TGCA'G Cuts at: 830 2760 2800 3239 830 Size: 1930 40 439 3478 Fragments arranged by size: 3478 1930 439 40 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2356 2356 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 2924 4135 5847 2924 Size: 1211 1712 2964 Fragments arranged by size: 2964 1712 1211 SfcI C'TryA_G Cuts at: 826 1045 2756 2796 3235 4370 5051 5242 826 Size: 219 1711 40 439 1135 681 191 1471 Fragments arranged by size: 1711 1471 1135 681 439 219 191 40 SfoI GGC'GCC Cuts at: 2431 2431 Size: 5887 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2855 3991 4892 5154 820 Size: 197 926 912 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 912 901 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2915 1517 Size: 1398 4489 StyI C'CwwG_G Cuts at: 513 1065 2160 2346 3171 3326 513 Size: 552 1095 186 825 155 3074 Fragments arranged by size: 3074 1095 825 552 186 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2276 3914 3931 4084 4269 4714 325 Size: 1255 696 1638 17 153 185 445 1498 Fragments arranged by size: 1638 1498 1255 696 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2922 4133 5845 Size: 274 80 33 51 1443 945 1211 1712 Cuts at: 5845 96 Size: 138 Fragments arranged by size: 1712 1443 1211 945 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 2353 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2367 4016 2367 Size: 1649 4238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI