(Circular) (Six-base) MAPSORT of: pFN21AB4161.seq Check: 4543 from: 1 to: 6118 pFN21AB4161 6118 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2872 2872 Size: 6118 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2219 3461 1937 Size: 282 1242 4594 Fragments arranged by size: 4594 1242 282 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4181 5565 5833 1632 Size: 2549 1384 268 1917 Fragments arranged by size: 2549 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3191 820 Size: 197 2174 3747 Fragments arranged by size: 3747 2174 197 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2051 2083 2051 Size: 32 6086 AlwNI CAG_nnn'CTG Cuts at: 2296 2497 3074 3861 5433 2296 Size: 201 577 787 1572 2981 Fragments arranged by size: 2981 1572 787 577 201 ApaI G_GGCC'C Cuts at: 2572 2984 2572 Size: 412 5706 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2345 3011 3427 3653 3805 5914 1493 Size: 852 666 416 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 666 416 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2577 3147 3273 3297 3443 1870 Size: 73 634 570 126 24 146 4545 Fragments arranged by size: 4545 634 570 146 126 73 24 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3236 3315 3411 3439 4894 Size: 325 217 627 1449 79 96 28 1455 Cuts at: 4894 618 Size: 1842 Fragments arranged by size: 1842 1455 1449 627 325 217 96 79 28 BanII G_rGCy'C Cuts at: 1391 2572 2984 3251 3437 1391 Size: 1181 412 267 186 4072 Fragments arranged by size: 4072 1181 412 267 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3194 928 Size: 971 1295 3852 Fragments arranged by size: 3852 1295 971 BbvCI CC'TCA_GC Cuts at: 2388 2388 Size: 6118 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2795 4010 5220 2795 Size: 1215 1210 3693 Fragments arranged by size: 3693 1215 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2572 2984 4179 5335 1790 Size: 782 412 1195 1156 2573 Fragments arranged by size: 2573 1195 1156 782 412 BmgBI CAC'GTC Cuts at: 1318 2473 1318 Size: 1155 4963 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2655 3849 4807 Size: 416 53 83 399 24 1238 1194 958 Cuts at: 4807 442 Size: 1753 Fragments arranged by size: 1753 1238 1194 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3096 4778 1239 Size: 1857 1682 2579 Fragments arranged by size: 2579 1857 1682 Bpu10I CC'TnA_GC Cuts at: 2388 2388 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2737 4243 5108 5406 2737 Size: 1506 865 298 3449 Fragments arranged by size: 3449 1506 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2451 2481 1178 Size: 30 165 30 1048 30 4815 Fragments arranged by size: 4815 1048 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2151 2740 2760 2763 2766 2805 2817 Size: 874 91 589 20 3 3 39 12 Cuts at: 2817 3175 1186 Size: 358 4129 Fragments arranged by size: 4129 874 589 358 91 39 20 12 3 3 BseYI C'CCAG_C Cuts at: 5321 5817 5321 Size: 496 5622 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3105 1163 Size: 573 1369 4176 Fragments arranged by size: 4176 1369 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3251 4179 4264 5335 3251 Size: 928 85 1071 4034 Fragments arranged by size: 4034 1071 928 85 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 BsmBI CGTCTCn'nnnn_ Cuts at: 3104 3104 Size: 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2572 2984 3251 3437 4179 4264 5335 Size: 399 782 412 267 186 742 85 1071 Cuts at: 5335 1391 Size: 2174 Fragments arranged by size: 2174 1071 782 742 412 399 267 186 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 3024 4007 5737 3024 Size: 983 1730 3405 Fragments arranged by size: 3405 1730 983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2872 3473 844 Size: 2028 601 3489 Fragments arranged by size: 3489 2028 601 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3126 4613 4787 65 Size: 1183 1878 1487 174 1396 Fragments arranged by size: 1878 1487 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2479 3291 3408 4762 4949 1700 Size: 110 669 812 117 1354 187 2869 Fragments arranged by size: 2869 1354 812 669 187 117 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 2643 4178 5190 2643 Size: 1535 1012 3571 Fragments arranged by size: 3571 1535 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2803 794 Size: 2009 4109 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 3282 1184 Size: 2098 4020 BstYI r'GATC_y Cuts at: 1972 3322 3448 3815 4200 4217 5669 5755 5767 Size: 1350 126 367 385 17 1452 86 12 Cuts at: 5767 6113 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1350 385 367 346 126 86 17 12 BstZ17I GTA'TAC Cuts at: 2220 2220 Size: 6118 BtgI C'CryG_G Cuts at: 513 1065 2564 2962 513 Size: 552 1499 398 3669 Fragments arranged by size: 3669 1499 552 398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3251 487 Size: 683 56 2025 3354 Fragments arranged by size: 3354 2025 683 56 BtsI GCAGTG_nn' Cuts at: 3058 3634 4428 4448 3058 Size: 576 794 20 4728 Fragments arranged by size: 4728 794 576 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2368 2481 3565 Size: 54 1425 50 275 18 538 113 1084 Cuts at: 3565 4454 6083 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1084 889 538 275 113 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 4048 1353 Size: 1903 792 3423 Fragments arranged by size: 3423 1903 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3249 3249 Size: 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3096 4181 4778 5565 5833 1239 Size: 393 1464 1085 597 787 268 1524 Fragments arranged by size: 1524 1464 1085 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2059 2764 2915 2981 2059 Size: 705 151 66 5196 Fragments arranged by size: 5196 705 151 66 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 EcoRV GAT'ATC Cuts at: 3022 3022 Size: 6118 FseI GG_CCGG'CC Cuts at: 2483 2483 Size: 6118 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4759 4791 4833 4865 1373 Size: 32 135 32 3187 32 42 32 2626 Fragments arranged by size: 3187 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 2755 748 Size: 2007 4111 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3105 3717 3105 Size: 612 5506 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 5232 5416 1305 Size: 1104 2823 184 2007 Fragments arranged by size: 2823 2007 1104 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2370 10 Size: 54 1425 343 538 3758 Fragments arranged by size: 3758 1425 538 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4076 4435 4594 518 Size: 648 2910 359 159 2042 Fragments arranged by size: 2910 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2296 3534 4211 5359 5604 1606 Size: 690 1238 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 690 677 245 NaeI GCC'GGC Cuts at: 2481 2481 Size: 6118 NcoI C'CATG_G Cuts at: 513 1065 2962 513 Size: 552 1897 3669 Fragments arranged by size: 3669 1897 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NgoMIV G'CCGG_C Cuts at: 2479 2479 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2849 1348 Size: 1501 4617 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PfoI T'CCnGG_A Cuts at: 3254 3254 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2764 2059 Size: 705 5413 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspOMI G'GGCC_C Cuts at: 2568 2980 2568 Size: 412 5706 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2296 2296 Size: 6118 SacI G_AGCT'C Cuts at: 3251 3251 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SanDI GG'GwC_CC Cuts at: 2764 2764 Size: 6118 SapI GCTCTTCn'nnn_ Cuts at: 3256 3256 Size: 6118 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 2224 2316 2907 2994 3226 3466 4601 Size: 219 1179 92 591 87 232 240 1135 Cuts at: 4601 5282 5473 826 Size: 681 191 1471 Fragments arranged by size: 1471 1179 1135 681 591 240 232 219 191 92 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3149 3275 3299 3445 1872 Size: 1277 126 24 146 4545 Fragments arranged by size: 4545 1277 146 126 24 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 3191 4222 5123 5385 820 Size: 197 926 809 439 1031 901 262 1553 Fragments arranged by size: 1553 1031 926 901 809 439 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 2942 1517 Size: 1425 4693 StyI C'CwwG_G Cuts at: 513 1065 2962 3557 513 Size: 552 1897 595 3074 Fragments arranged by size: 3074 1897 595 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3224 4145 4162 4315 4500 4945 325 Size: 1255 1644 921 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1255 921 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4364 6076 96 Size: 274 80 33 51 1443 2387 1712 138 Fragments arranged by size: 2387 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2451 2483 6078 6110 2451 Size: 32 3595 32 2459 Fragments arranged by size: 3595 2459 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3147 3273 3297 3443 1870 Size: 1277 126 24 146 4545 Fragments arranged by size: 4545 1277 146 126 24 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI Bsu36I DraIII EcoNI FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI