Restriction map for
pFN21AB4161
(Linear) (Six-base) MAPSORT of: KIBB4161.seq Check: 5892 from: 1 to: 1413
KIBB4161.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 867 1413
Size: 867 546
AccI GT'mk_AC
Cuts at: 0 214 1413
Size: 214 1199
AflII C'TTAA_G
Cuts at: 0 1186 1413
Size: 1186 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 1413
Size: 46 32 1335
Fragments arranged by size:
1335 46 32
AlwNI CAG_nnn'CTG
Cuts at: 0 291 492 1069 1413
Size: 291 201 577 344
Fragments arranged by size:
577 344 291 201
ApaI G_GGCC'C
Cuts at: 0 567 979 1413
Size: 567 412 434
Fragments arranged by size:
567 434 412
ApoI r'AATT_y
Cuts at: 0 340 1006 1413
Size: 340 666 407
Fragments arranged by size:
666 407 340
AvaI C'yCGr_G
Cuts at: 0 572 1142 1268 1292 1413
Size: 572 570 126 24 121
Fragments arranged by size:
572 570 126 121 24
BanI G'GyrC_C
Cuts at: 0 1231 1310 1406 1413
Size: 1231 79 96 7
Fragments arranged by size:
1231 96 79 7
BanII G_rGCy'C
Cuts at: 0 567 979 1246 1413
Size: 567 412 267 167
Fragments arranged by size:
567 412 267 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 1189 1413
Size: 1189 224
BbvCI CC'TCA_GC
Cuts at: 0 383 1413
Size: 383 1030
BciVI GTATCCnnnnn_n'
Cuts at: 0 790 1413
Size: 790 623
Bme1580I G_kGCm'C
Cuts at: 0 567 979 1413
Size: 567 412 434
Fragments arranged by size:
567 434 412
BmgBI CAC'GTC
Cuts at: 0 468 1413
Size: 468 945
BmrI ACTGGGnnnn_n'
Cuts at: 0 650 1413
Size: 650 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1413
Size: 1091 322
Bpu10I CC'TnA_GC
Cuts at: 0 383 1413
Size: 383 1030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1413
Size: 732 681
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 446 476 1413
Size: 446 30 937
Fragments arranged by size:
937 446 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 146 735 755 758 761 800 812
Size: 55 91 589 20 3 3 39 12
Cuts at: 812 1170 1413
Size: 358 243
Fragments arranged by size:
589 358 243 91 55 39 20 12
3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1413
Size: 1100 313
BsiHKAI G_wGCw'C
Cuts at: 0 1246 1413
Size: 1246 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1099 1413
Size: 1099 314
Bsp1286I G_dGCh'C
Cuts at: 0 567 979 1246 1413
Size: 567 412 267 167
Fragments arranged by size:
567 412 267 167
BspHI T'CATG_A
Cuts at: 0 1019 1413
Size: 1019 394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 867 1413
Size: 867 546
BsrDI GCAATG_nn'
Cuts at: 0 1121 1413
Size: 1121 292
BsrFI r'CCGG_y
Cuts at: 0 474 1286 1403 1413
Size: 474 812 117 10
Fragments arranged by size:
812 474 117 10
BssSI C'ACGA_G
Cuts at: 0 638 1413
Size: 638 775
BstAPI GCAn_nnn'nTGC
Cuts at: 0 798 1413
Size: 798 615
BstEII G'GTnAC_C
Cuts at: 0 1277 1413
Size: 1277 136
BstYI r'GATC_y
Cuts at: 0 1317 1413
Size: 1317 96
BstZ17I GTA'TAC
Cuts at: 0 215 1413
Size: 215 1198
BtgI C'CryG_G
Cuts at: 0 559 957 1413
Size: 559 398 456
Fragments arranged by size:
559 456 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1246 1413
Size: 1246 167
BtsI GCAGTG_nn'
Cuts at: 0 1053 1413
Size: 1053 360
EaeI y'GGCC_r
Cuts at: 0 363 476 1413
Size: 363 113 937
Fragments arranged by size:
937 363 113
EarI CTCTTCn'nnn_
Cuts at: 0 1251 1413
Size: 1251 162
EcoICRI GAG'CTC
Cuts at: 0 1244 1413
Size: 1244 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1413
Size: 1091 322
EcoO109I rG'GnC_Cy
Cuts at: 0 54 759 910 976 1413
Size: 54 705 151 66 437
Fragments arranged by size:
705 437 151 66 54
EcoRV GAT'ATC
Cuts at: 0 1017 1413
Size: 1017 396
FseI GG_CCGG'CC
Cuts at: 0 478 1413
Size: 478 935
HindIII A'AGCT_T
Cuts at: 0 750 1413
Size: 750 663
MfeI C'AATT_G
Cuts at: 0 1100 1413
Size: 1100 313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1413
Size: 404 1009
MscI TGG'CCA
Cuts at: 0 365 1413
Size: 365 1048
MspA1I CmG'CkG
Cuts at: 0 291 1413
Size: 291 1122
NaeI GCC'GGC
Cuts at: 0 476 1413
Size: 476 937
NcoI C'CATG_G
Cuts at: 0 957 1413
Size: 957 456
NgoMIV G'CCGG_C
Cuts at: 0 474 1413
Size: 474 939
PasI CC'CwG_GG
Cuts at: 0 844 1413
Size: 844 569
PfoI T'CCnGG_A
Cuts at: 0 1249 1413
Size: 1249 164
PpuMI rG'GwC_Cy
Cuts at: 0 54 759 1413
Size: 54 705 654
Fragments arranged by size:
705 654 54
PspOMI G'GGCC_C
Cuts at: 0 563 975 1413
Size: 563 412 438
Fragments arranged by size:
563 438 412
PvuII CAG'CTG
Cuts at: 0 291 1413
Size: 291 1122
SacI G_AGCT'C
Cuts at: 0 1246 1413
Size: 1246 167
SanDI GG'GwC_CC
Cuts at: 0 759 1413
Size: 759 654
SapI GCTCTTCn'nnn_
Cuts at: 0 1251 1413
Size: 1251 162
SfcI C'TryA_G
Cuts at: 0 219 311 902 989 1221 1413
Size: 219 92 591 87 232 192
Fragments arranged by size:
591 232 219 192 92 87
SmaI CCC'GGG
Cuts at: 0 1144 1270 1294 1413
Size: 1144 126 24 119
Fragments arranged by size:
1144 126 119 24
SmlI C'TyrA_G
Cuts at: 0 747 1186 1413
Size: 747 439 227
Fragments arranged by size:
747 439 227
StuI AGG'CCT
Cuts at: 0 937 1413
Size: 937 476
StyI C'CwwG_G
Cuts at: 0 957 1413
Size: 957 456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1219 1413
Size: 1219 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 446 478 1413
Size: 446 32 935
Fragments arranged by size:
935 446 32
XmaI C'CCGG_G
Cuts at: 0 1142 1268 1292 1413
Size: 1142 126 24 121
Fragments arranged by size:
1142 126 121 24
Enzymes that do cut:
AarI AccI AflII AloI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BciVI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstEII
BstYI BstZ17I BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRV FseI HindIII MfeI MmeI MscI
MspA1I NaeI NcoI NgoMIV PasI PfoI PpuMI PspOMI
PvuII SacI SanDI SapI SfcI SmaI SmlI StuI
StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BclI BfrBI BglI BglII BlpI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BspEI BsrBI BsrGI BssHII BstBI
BstXI Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI FalI FspI FspAI HaeII Hin4I
HincII HpaI KasI KpnI MluI MslI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI