(Circular) (Six-base) MAPSORT of: pFN21AB4120.seq Check: 392 from: 1 to: 6058 pFN21AB4120 6058 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2392 2392 Size: 6058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3868 278 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 1937 Size: 1464 4594 Acc65I G'GTAC_C Cuts at: 3379 3379 Size: 6058 AclI AA'CG_TT Cuts at: 1563 4185 4558 1563 Size: 2622 373 3063 Fragments arranged by size: 3063 2622 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4121 5505 5773 1632 Size: 2489 1384 268 1917 Fragments arranged by size: 2489 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5861 AflIII A'CryG_T Cuts at: 1203 4957 5934 1203 Size: 3754 977 1327 Fragments arranged by size: 3754 1327 977 AgeI A'CCGG_T Cuts at: 4889 4889 Size: 6058 AhdI GACnn_n'nnGTC Cuts at: 4787 4787 Size: 6058 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6058 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2515 2547 2515 Size: 32 6026 AlwNI CAG_nnn'CTG Cuts at: 2080 2230 2840 3801 5373 2080 Size: 150 610 961 1572 2765 Fragments arranged by size: 2765 1572 961 610 150 ApaI G_GGCC'C Cuts at: 3163 3163 Size: 6058 ApaLI G'TGCA_C Cuts at: 4115 5271 4115 Size: 1156 4902 ApoI r'AATT_y Cuts at: 1493 2992 3367 3593 3745 5854 1493 Size: 1499 375 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 375 226 152 AseI AT'TA_AT Cuts at: 160 1032 4612 160 Size: 872 3580 1606 Fragments arranged by size: 3580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2827 3383 1870 Size: 73 142 742 556 4545 Fragments arranged by size: 4545 742 556 142 73 BamHI G'GATC_C Cuts at: 3148 3388 3755 3148 Size: 240 367 5451 Fragments arranged by size: 5451 367 240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 4834 618 Size: 325 217 627 1592 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2834 3163 3377 1391 Size: 1443 329 214 4072 Fragments arranged by size: 4072 1443 329 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 928 Size: 971 360 4727 Fragments arranged by size: 4727 971 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2858 2892 4249 4283 1903 Size: 34 921 34 1357 34 3678 Fragments arranged by size: 3678 1357 921 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2909 3950 5160 2909 Size: 1041 1210 3807 Fragments arranged by size: 3807 1210 1041 BclI T'GATC_A Cuts at: 1548 1548 Size: 6058 BfrBI ATG'CAT Cuts at: 2204 2204 Size: 6058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4669 136 Size: 107 122 71 631 605 2997 1525 Fragments arranged by size: 2997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6053 6053 Size: 6058 BlpI GC'TnA_GC Cuts at: 2833 2955 3475 2833 Size: 122 520 5416 Fragments arranged by size: 5416 520 122 Bme1580I G_kGCm'C Cuts at: 1790 3163 4119 5275 1790 Size: 1373 956 1156 2573 Fragments arranged by size: 2573 1373 1156 956 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2588 2842 3789 Size: 416 53 83 399 24 1171 254 947 Cuts at: 3789 4747 442 Size: 958 1753 Fragments arranged by size: 1753 1171 958 947 416 399 254 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 4718 1239 Size: 968 2511 2579 Fragments arranged by size: 2579 2511 968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4183 5048 5346 4183 Size: 865 298 4895 Fragments arranged by size: 4895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 2701 4721 882 Size: 617 645 557 2020 2219 Fragments arranged by size: 2219 2020 645 617 557 BsaAI yAC'GTr Cuts at: 493 1204 3849 493 Size: 711 2645 2702 Fragments arranged by size: 2702 2645 711 BsaBI GATnn'nnATC Cuts at: 3147 6052 3147 Size: 2905 3153 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 4247 4882 275 Size: 53 83 186 936 2332 382 635 1451 Fragments arranged by size: 2332 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4491 4889 5163 5310 5721 4491 Size: 398 274 147 411 4828 Fragments arranged by size: 4828 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2515 2545 1178 Size: 30 165 30 624 30 458 30 4691 Fragments arranged by size: 4691 624 458 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2048 2162 3119 1186 Size: 859 3 114 957 4125 Fragments arranged by size: 4125 957 859 114 3 BseYI C'CCAG_C Cuts at: 5261 5757 5261 Size: 496 5562 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3508 4269 5297 1540 Size: 275 186 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4119 4204 5275 4119 Size: 85 1071 4902 Fragments arranged by size: 4902 1071 85 BsmI GAATG_Cn' Cuts at: 2778 3569 3662 2778 Size: 791 93 5174 Fragments arranged by size: 5174 791 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2834 3163 3377 4119 4204 5275 1391 Size: 399 1044 329 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 1044 742 399 329 214 85 BspEI T'CCGG_A Cuts at: 5721 5721 Size: 6058 BspHI T'CATG_A Cuts at: 3947 5677 3947 Size: 1730 4328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2392 3413 844 Size: 1548 1021 3489 Fragments arranged by size: 3489 1548 1021 BsrBI CCG'CTC Cuts at: 1394 1823 3945 1394 Size: 429 2122 3507 Fragments arranged by size: 3507 2122 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2784 4553 4727 65 Size: 1183 1536 1769 174 1396 Fragments arranged by size: 1769 1536 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4702 4889 1700 Size: 110 2892 187 2869 Fragments arranged by size: 2892 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6058 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6058 BssSI C'ACGA_G Cuts at: 4118 5130 4118 Size: 1012 5046 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6058 BstBI TT'CG_AA Cuts at: 4862 4862 Size: 6058 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6058 BstYI r'GATC_y Cuts at: 1972 2037 3148 3388 3755 4140 4157 5609 5695 Size: 65 1111 240 367 385 17 1452 86 Cuts at: 5695 5707 6053 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1111 385 367 346 240 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2455 513 Size: 552 939 451 4116 Fragments arranged by size: 4116 939 552 451 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5319 Fragments arranged by size: 5319 683 56 BtsI GCAGTG_nn' Cuts at: 3574 4368 4388 3574 Size: 794 20 5244 Fragments arranged by size: 5244 794 20 ClaI AT'CG_AT Cuts at: 1554 2427 1554 Size: 873 5185 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6023 DraI TTT'AAA Cuts at: 2700 3362 2700 Size: 662 5396 DraIII CAC_nnn'GTG Cuts at: 2723 2723 Size: 6058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5065 809 Size: 507 3749 1802 Fragments arranged by size: 3749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 3505 4394 Size: 54 1425 50 275 18 378 1297 889 Cuts at: 4394 6023 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1297 889 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3505 1537 Size: 275 1693 4090 Fragments arranged by size: 4090 1693 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2317 3988 1353 Size: 964 1671 3423 Fragments arranged by size: 3423 1671 964 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4645 5019 5165 387 Size: 1064 138 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 4121 4718 5505 5773 1239 Size: 393 575 1914 597 787 268 1524 Fragments arranged by size: 1914 1524 787 597 575 393 268 EcoO109I rG'GnC_Cy Cuts at: 3160 3160 Size: 6058 EcoRI G'AATT_C Cuts at: 3367 3745 3367 Size: 378 5680 EcoRV GAT'ATC Cuts at: 2236 2874 2236 Size: 638 5420 FspI TGC'GCA Cuts at: 4564 4564 Size: 6058 HaeII r_GCGC'y Cuts at: 1678 2060 2401 5205 1678 Size: 382 341 2804 2531 Fragments arranged by size: 2804 2531 382 341 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2512 2544 3212 Size: 32 135 32 455 32 453 32 668 Cuts at: 3212 3244 4699 4731 4773 4805 1373 Size: 32 1455 32 42 32 2626 Fragments arranged by size: 2626 1455 668 455 453 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3402 3648 669 Size: 988 281 1464 246 3079 Fragments arranged by size: 3079 1464 988 281 246 HindIII A'AGCT_T Cuts at: 748 2139 748 Size: 1391 4667 HpaI GTT'AAC Cuts at: 3648 3648 Size: 6058 KpnI G_GTAC'C Cuts at: 3383 3383 Size: 6058 MfeI C'AATT_G Cuts at: 2997 3657 2997 Size: 660 5398 MluI A'CGCG_T Cuts at: 5934 5934 Size: 6058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3035 5172 5356 1305 Size: 1730 2137 184 2007 Fragments arranged by size: 2137 2007 1730 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 3858 Fragments arranged by size: 3858 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 2967 3047 4016 4375 4534 518 Size: 648 1623 178 80 969 359 159 2042 Fragments arranged by size: 2042 1623 969 648 359 178 159 80 MspA1I CmG'CkG Cuts at: 1606 3474 4151 5299 5544 1606 Size: 1868 677 1148 245 2120 Fragments arranged by size: 2120 1868 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2455 513 Size: 552 939 451 4116 Fragments arranged by size: 4116 939 552 451 NdeI CA'TA_TG Cuts at: 387 3136 387 Size: 2749 3309 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6058 NotI GC'GGCC_GC Cuts at: 3505 3505 Size: 6058 NsiI A_TGCA'T Cuts at: 2206 2206 Size: 6058 NspI r_CATG'y Cuts at: 1132 1229 3416 4961 1132 Size: 97 2187 1545 2229 Fragments arranged by size: 2229 2187 1545 97 PasI CC'CwG_GG Cuts at: 1348 3320 1348 Size: 1972 4086 PciI A'CATG_T Cuts at: 4957 4957 Size: 6058 PflMI CCAn_nnn'nTGG Cuts at: 1108 2230 1108 Size: 1122 4936 PmeI GTTT'AAAC Cuts at: 3362 3362 Size: 6058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2515 2547 4127 4159 5640 5672 2515 Size: 32 1580 32 1481 32 2901 Fragments arranged by size: 2901 1580 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6058 PsiI TTA'TAA Cuts at: 2598 3260 3628 2598 Size: 662 368 5028 Fragments arranged by size: 5028 662 368 PspOMI G'GGCC_C Cuts at: 3159 3159 Size: 6058 PstI C_TGCA'G Cuts at: 830 2019 3115 3410 830 Size: 1189 1096 295 3478 Fragments arranged by size: 3478 1189 1096 295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6058 SalI G'TCGA_C Cuts at: 1936 3400 1936 Size: 1464 4594 SbfI CC_TGCA'GG Cuts at: 3410 3410 Size: 6058 ScaI AGT'ACT Cuts at: 4306 6018 4306 Size: 1712 4346 SfcI C'TryA_G Cuts at: 826 1045 2015 2124 3111 3406 4541 5222 5413 Size: 219 970 109 987 295 1135 681 191 Cuts at: 5413 826 Size: 1471 Fragments arranged by size: 1471 1135 987 970 681 295 219 191 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6058 SmaI CCC'GGG Cuts at: 1872 2829 3385 1872 Size: 957 556 4545 Fragments arranged by size: 4545 957 556 SmlI C'TyrA_G Cuts at: 820 1017 1943 4162 5063 5325 820 Size: 197 926 2219 901 262 1553 Fragments arranged by size: 2219 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3849 493 Size: 3356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6058 SphI G_CATG'C Cuts at: 1132 3416 1132 Size: 2284 3774 SspI AAT'ATT Cuts at: 5 52 3982 5 Size: 47 3930 2081 Fragments arranged by size: 3930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6058 StyI C'CwwG_G Cuts at: 513 1065 2004 2455 2711 3497 513 Size: 552 939 451 256 786 3074 Fragments arranged by size: 3074 939 786 552 451 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2824 4085 4102 4255 4440 4885 325 Size: 1255 1244 1261 17 153 185 445 1498 Fragments arranged by size: 1498 1261 1255 1244 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2218 4304 6016 Size: 274 80 33 51 1443 241 2086 1712 Cuts at: 6016 96 Size: 138 Fragments arranged by size: 2086 1712 1443 274 241 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2707 2739 6018 6050 2707 Size: 32 3279 32 2715 Fragments arranged by size: 3279 2715 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6058 XbaI T'CTAG_A Cuts at: 3394 3394 Size: 6058 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6058 XmaI C'CCGG_G Cuts at: 1870 2827 3383 1870 Size: 957 556 4545 Fragments arranged by size: 4545 957 556 XmnI GAAnn'nnTTC Cuts at: 4187 4187 Size: 6058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3866 276 Size: 53 83 186 936 2332 2468 Fragments arranged by size: 2468 2332 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI