Restriction map for
pFN21AB4091
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BclI BcnI BfrI
BglI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BspDI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
Cac8I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1261 GTATAC GT^[AC][GT]AC
AciI 1407 GCGG CCGC(-3/-1)
AflIII 1040 ACATGT A^C[GA][CT]GT
Aor51HI 5 AGCGCT AGC^GCT
AspHI 542 GAGCTC G[AT]GC[AT]^C
AvaI 323 CCCGAG C^[CT]CG[GA]G
BalI 1107 TGGCCA TGG^CCA
BanI 790 GGCACC G^G[CT][GA]CC
BcgI 904 CGAAGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 179 CTAG C^TAG
BglII 405 AGATCT A^GATCT
BlpI 36 GCTCAGC GC^T[ACGT]AGC
BpmI 1011 CTCCAG CTGGAG(16/14)
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
BsaBI 79 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1432 GAGGAG GAGGAG(10/8)
BsiHKAI 542 GAGCTC G[AT]GC[AT]^C
BsoBI 323 CCCGAG C^[CT]CG[GA]G
BspLU11I 1040 ACATGT A^CATGT
BspMI 223 GCAGGT ACCTGC(4/8)
Bst1107I 1261 GTATAC GTA^TAC
BstF5I 1269 GGATG GGATG(2/0)
BstZ17I 1261 GTATAC GTA^TAC
CelII 36 GCTCAGC GC^T[ACGT]AGC
CfoI 6 GCGC GCG^C
DraII 225 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 640 CCGTGG C^C[GA][CT]GG
Eco47III 5 AGCGCT AGC^GCT
EcoO109I 225 AGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 1269 GGATG GGATG(9/13)
HaeII 5 AGCGCT [GA]GCGC^[CT]
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
MaeI 179 CTAG C^TAG
MaeII 582 ACGT A^CGT
MamI 79 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1107 TGGCCA TGG^CCA
MscI 1107 TGGCCA TGG^CCA
MslI 1035 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 617 CAGCTG C[AC]G^C[GT]G
NdeI 839 CATATG CA^TATG
NspI 1040 ACATGT [GA]CATG^[CT]
PpuMI 225 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 620 CTGCAG CTGCA^G
PvuII 617 CAGCTG CAG^CTG
SacI 542 GAGCTC GAGCT^C
XcmI 794 CCAATGAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 34 GGGCTC G[GA]GC[CT]^C
542 GAGCTC
BbsI 799 GAAGAC GAAGAC(2/6)
905 GAAGAC
BciVI 291 GGATAC GTATCC(6/5)
567 GTATCC
BpuAI 799 GAAGAC GAAGAC(2/6)
905 GAAGAC
BsgI 231 CTGCAC GTGCAG(16/14)
1425 CTGCAC
BsiYI 52 CCCCAGTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1385 CCTGGTACGGG
BslI 52 CCCCAGTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1385 CCTGGTACGGG
BsmI 364 GAATGC GAATGC(1/-1)
1092 GAATGC
BstYI 405 AGATCT [GA]^GATC[CT]
1140 GGATCT
EaeI 637 TGGCCG [CT]^GGCC[GA]
1107 TGGCCA
HphI 67 GGTGA GGTGA(8/7)
537 TCACC
MflI 405 AGATCT [GA]^GATC[CT]
1140 GGATCT
MseI 332 TTAA T^TAA
587 TTAA
PleI 554 GACTC GAGTC(4/5)
884 GACTC
SfcI 620 CTGCAG C^T[GA][CT]AG
747 CTACAG
TaqI 903 TCGA T^CGA
1340 TCGA
Tru9I 332 TTAA T^TAA
587 TTAA
Tsp45I 1122 GTCAC ^GT[GC]AC
1162 GTGAC
TthHB8I 903 TCGA T^CGA
1340 TCGA
XhoII 405 AGATCT [GA]^GATC[CT]
1140 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BmyI BsaJI BsmFI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI HaeIII HinfI
ItaI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SspBI StyI TfiI TseI Tsp509I
TspRI