(Circular) (Six-base) MAPSORT of: pFN21AB4091.seq Check: 745 from: 1 to: 6145 pFN21AB4091 6145 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3267 3488 1937 Size: 1330 221 4594 Fragments arranged by size: 4594 1330 221 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2208 3010 4208 5592 5860 1632 Size: 576 802 1198 1384 268 1917 Fragments arranged by size: 1917 1384 1198 802 576 268 AfeI AGC'GCT Cuts at: 2012 2012 Size: 6145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 3045 5044 6021 1203 Size: 1842 1999 977 1327 Fragments arranged by size: 1999 1842 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2251 1184 Size: 1067 5078 AlwNI CAG_nnn'CTG Cuts at: 3888 5460 3888 Size: 1572 4573 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 2447 2659 3310 3454 3680 3832 5941 1493 Size: 954 212 651 144 226 152 2109 1697 Fragments arranged by size: 2109 1697 954 651 226 212 152 144 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2328 3470 1870 Size: 73 385 1142 4545 Fragments arranged by size: 4545 1142 385 73 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2795 3466 4921 618 Size: 325 217 627 1008 671 1455 1842 Fragments arranged by size: 1842 1455 1008 671 627 325 217 BanII G_rGCy'C Cuts at: 1391 2043 2551 3464 1391 Size: 652 508 913 4072 Fragments arranged by size: 4072 913 652 508 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2811 2917 928 Size: 971 912 106 4156 Fragments arranged by size: 4156 971 912 106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2898 2932 4336 4370 1903 Size: 34 961 34 1404 34 3678 Fragments arranged by size: 3678 1404 961 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2290 2583 4037 5247 2290 Size: 293 1454 1210 3188 Fragments arranged by size: 3188 1454 1210 293 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2410 6140 2410 Size: 3730 2415 BlpI GC'TnA_GC Cuts at: 2042 3562 2042 Size: 1520 4625 Bme1580I G_kGCm'C Cuts at: 1790 2441 4206 5362 1790 Size: 651 1765 1156 2573 Fragments arranged by size: 2573 1765 1156 651 BmgBI CAC'GTC Cuts at: 1318 2588 1318 Size: 1270 4875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 2498 3876 Size: 416 53 83 399 24 636 445 1378 Cuts at: 3876 4834 442 Size: 958 1753 Fragments arranged by size: 1753 1378 958 636 445 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3001 4805 1239 Size: 1762 1804 2579 Fragments arranged by size: 2579 1804 1762 Bpu10I CC'TnA_GC Cuts at: 2479 2479 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 2088 6139 2088 Size: 4051 2094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3952 4334 4969 275 Size: 53 83 186 936 2419 382 635 1451 Fragments arranged by size: 2419 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5920 Fragments arranged by size: 5920 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3452 1186 Size: 2266 3879 BseYI C'CCAG_C Cuts at: 3424 5348 5844 3424 Size: 1924 496 3725 Fragments arranged by size: 3725 1924 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2221 3415 1163 Size: 573 485 1194 3893 Fragments arranged by size: 3893 1194 573 485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2551 4206 4291 5362 2551 Size: 1655 85 1071 3334 Fragments arranged by size: 3334 1655 1071 85 BsmI GAATG_Cn' Cuts at: 2375 3103 3656 3749 2375 Size: 728 553 93 4771 Fragments arranged by size: 4771 728 553 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2441 2551 3464 4206 4291 5362 Size: 399 253 398 110 913 742 85 1071 Cuts at: 5362 1391 Size: 2174 Fragments arranged by size: 2174 1071 913 742 399 398 253 110 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2219 3500 844 Size: 1375 1281 3489 Fragments arranged by size: 3489 1375 1281 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2784 2914 3250 3407 4640 4814 65 Size: 1183 1536 130 336 157 1233 174 1396 Fragments arranged by size: 1536 1396 1233 1183 336 174 157 130 BsrFI r'CCGG_y Cuts at: 1700 1810 4789 4976 1700 Size: 110 2979 187 2869 Fragments arranged by size: 2979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2405 2781 3208 96 Size: 2309 376 427 3033 Fragments arranged by size: 3033 2309 427 376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2410 3145 3475 3842 4227 4244 5696 5782 Size: 438 735 330 367 385 17 1452 86 Cuts at: 5782 5794 6140 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 735 438 385 367 346 330 86 17 12 BstZ17I GTA'TAC Cuts at: 3268 3268 Size: 6145 BtgI C'CryG_G Cuts at: 513 1065 2645 513 Size: 552 1580 4013 Fragments arranged by size: 4013 1580 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 3009 3661 4455 4475 3009 Size: 652 794 20 4679 Fragments arranged by size: 4679 794 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2377 2412 529 Size: 35 1813 35 4262 Fragments arranged by size: 4262 1813 35 35 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2642 3112 3592 Size: 54 1425 50 275 18 812 470 480 Cuts at: 3592 4481 6110 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 812 480 470 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4075 1353 Size: 2722 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2549 2549 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 3001 3010 4208 4805 5592 5860 Size: 393 576 793 9 1198 597 787 268 Cuts at: 5860 1239 Size: 1524 Fragments arranged by size: 1524 1198 793 787 597 576 393 268 9 EcoO109I rG'GnC_Cy Cuts at: 2231 2231 Size: 6145 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2014 5292 1678 Size: 336 3278 2531 Fragments arranged by size: 3278 2531 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2335 2367 4786 Size: 32 135 32 614 32 117 32 2419 Cuts at: 4786 4818 4860 4892 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2419 614 135 117 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3182 5259 5443 1305 Size: 1877 2077 184 2007 Fragments arranged by size: 2077 2007 1877 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3114 10 Size: 54 1425 343 1282 3041 Fragments arranged by size: 3041 1425 1282 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3044 4103 4462 4621 518 Size: 648 1878 1059 359 159 2042 Fragments arranged by size: 2042 1878 1059 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2624 3561 4238 5386 5631 1606 Size: 1018 937 677 1148 245 2120 Fragments arranged by size: 2120 1148 1018 937 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5593 NdeI CA'TA_TG Cuts at: 387 2845 387 Size: 2458 3687 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3049 3503 5048 1132 Size: 97 1820 454 1545 2229 Fragments arranged by size: 2229 1820 1545 454 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 3045 5044 3045 Size: 1999 4146 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2231 2231 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 2609 3715 2609 Size: 1106 5039 PstI C_TGCA'G Cuts at: 830 2629 3497 830 Size: 1799 868 3478 Fragments arranged by size: 3478 1799 868 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2624 2624 Size: 6145 SacI G_AGCT'C Cuts at: 2551 2551 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2625 2752 3493 4628 5309 5500 826 Size: 219 1580 127 741 1135 681 191 1471 Fragments arranged by size: 1580 1471 1135 741 681 219 191 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2698 3062 3383 3584 513 Size: 552 1633 364 321 201 3074 Fragments arranged by size: 3074 1633 552 364 321 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4172 4189 4342 4527 4972 325 Size: 1255 2592 17 153 185 445 1498 Fragments arranged by size: 2592 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2405 2781 3208 Size: 274 80 33 51 1443 428 376 427 Cuts at: 3208 4391 6103 96 Size: 1183 1712 138 Fragments arranged by size: 1712 1443 1183 428 427 376 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2230 2262 6105 6137 2230 Size: 32 3843 32 2238 Fragments arranged by size: 3843 2238 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2806 2806 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI