Restriction map for
pFN21AB4091
(Linear) (Six-base) MAPSORT of: KIBB4091.seq Check: 4886 from: 1 to: 1440
KIBB4091.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 1262 1440
Size: 1262 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1005 1440
Size: 203 802 435
Fragments arranged by size:
802 435 203
AfeI AGC'GCT
Cuts at: 0 7 1440
Size: 7 1433
AflIII A'CryG_T
Cuts at: 0 1040 1440
Size: 1040 400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 246 1440
Size: 246 1194
ApoI r'AATT_y
Cuts at: 0 442 654 1305 1440
Size: 442 212 651 135
Fragments arranged by size:
651 442 212 135
AvaI C'yCGr_G
Cuts at: 0 323 1440
Size: 323 1117
BanI G'GyrC_C
Cuts at: 0 790 1440
Size: 790 650
BanII G_rGCy'C
Cuts at: 0 38 546 1440
Size: 38 508 894
Fragments arranged by size:
894 508 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 912 1440
Size: 806 106 528
Fragments arranged by size:
806 528 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 893 927 1440
Size: 893 34 513
Fragments arranged by size:
893 513 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 285 578 1440
Size: 285 293 862
Fragments arranged by size:
862 293 285
BglII A'GATC_T
Cuts at: 0 405 1440
Size: 405 1035
BlpI GC'TnA_GC
Cuts at: 0 37 1440
Size: 37 1403
Bme1580I G_kGCm'C
Cuts at: 0 436 1440
Size: 436 1004
BmgBI CAC'GTC
Cuts at: 0 583 1440
Size: 583 857
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 493 1440
Size: 48 445 947
Fragments arranged by size:
947 445 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1440
Size: 996 444
Bpu10I CC'TnA_GC
Cuts at: 0 474 1440
Size: 474 966
BsaBI GATnn'nnATC
Cuts at: 0 83 1440
Size: 83 1357
BseYI C'CCAG_C
Cuts at: 0 1419 1440
Size: 1419 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1410 1440
Size: 216 1194 30
Fragments arranged by size:
1194 216 30
BsiHKAI G_wGCw'C
Cuts at: 0 546 1440
Size: 546 894
BsmI GAATG_Cn'
Cuts at: 0 370 1098 1440
Size: 370 728 342
Fragments arranged by size:
728 370 342
Bsp1286I G_dGCh'C
Cuts at: 0 38 436 546 1440
Size: 38 398 110 894
Fragments arranged by size:
894 398 110 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 214 1440
Size: 214 1226
BsrDI GCAATG_nn'
Cuts at: 0 779 909 1245 1402 1440
Size: 779 130 336 157 38
Fragments arranged by size:
779 336 157 130 38
BsrGI T'GTAC_A
Cuts at: 0 400 776 1203 1440
Size: 400 376 427 237
Fragments arranged by size:
427 400 376 237
BstYI r'GATC_y
Cuts at: 0 405 1140 1440
Size: 405 735 300
Fragments arranged by size:
735 405 300
BstZ17I GTA'TAC
Cuts at: 0 1263 1440
Size: 1263 177
BtgI C'CryG_G
Cuts at: 0 640 1440
Size: 640 800
BtsI GCAGTG_nn'
Cuts at: 0 1004 1440
Size: 1004 436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 372 407 1440
Size: 372 35 1033
Fragments arranged by size:
1033 372 35
EaeI y'GGCC_r
Cuts at: 0 637 1107 1440
Size: 637 470 333
Fragments arranged by size:
637 470 333
EcoICRI GAG'CTC
Cuts at: 0 544 1440
Size: 544 896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 996 1005 1440
Size: 203 793 9 435
Fragments arranged by size:
793 435 203 9
EcoO109I rG'GnC_Cy
Cuts at: 0 226 1440
Size: 226 1214
HaeII r_GCGC'y
Cuts at: 0 9 1440
Size: 9 1431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 330 362 1440
Size: 181 32 117 32 1078
Fragments arranged by size:
1078 181 117 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1440
Size: 1177 263
MscI TGG'CCA
Cuts at: 0 1109 1440
Size: 1109 331
MslI CAynn'nnrTG
Cuts at: 0 1039 1440
Size: 1039 401
MspA1I CmG'CkG
Cuts at: 0 619 1440
Size: 619 821
NdeI CA'TA_TG
Cuts at: 0 840 1440
Size: 840 600
NspI r_CATG'y
Cuts at: 0 1044 1440
Size: 1044 396
PciI A'CATG_T
Cuts at: 0 1040 1440
Size: 1040 400
PpuMI rG'GwC_Cy
Cuts at: 0 226 1440
Size: 226 1214
PsiI TTA'TAA
Cuts at: 0 604 1440
Size: 604 836
PstI C_TGCA'G
Cuts at: 0 624 1440
Size: 624 816
PvuII CAG'CTG
Cuts at: 0 619 1440
Size: 619 821
SacI G_AGCT'C
Cuts at: 0 546 1440
Size: 546 894
SfcI C'TryA_G
Cuts at: 0 620 747 1440
Size: 620 127 693
Fragments arranged by size:
693 620 127
StyI C'CwwG_G
Cuts at: 0 693 1057 1378 1440
Size: 693 364 321 62
Fragments arranged by size:
693 364 321 62
TatI w'GTAC_w
Cuts at: 0 400 776 1203 1440
Size: 400 376 427 237
Fragments arranged by size:
427 400 376 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 225 257 1440
Size: 225 32 1183
Fragments arranged by size:
1183 225 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 801 1440
Size: 801 639
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI ApoI AvaI BanI
BanII BbsI BcgI BciVI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaBI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrGI BstYI BstZ17I BtgI BtsI
CspCI EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI
MscI MslI MspA1I NdeI NspI PciI PpuMI PsiI
PstI PvuII SacI SfcI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BglI BmtI
BplI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI