(Circular) (Six-base) MAPSORT of: pFN21AB4044.seq Check: 736 from: 1 to: 6253 pFN21AB4044 6253 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2598 2598 Size: 6253 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4063 278 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3596 1937 Size: 1659 4594 Acc65I G'GTAC_C Cuts at: 3574 3574 Size: 6253 AclI AA'CG_TT Cuts at: 1563 4380 4753 1563 Size: 2817 373 3063 Fragments arranged by size: 3063 2817 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4316 5700 5968 1632 Size: 2684 1384 268 1917 Fragments arranged by size: 2684 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6056 AflIII A'CryG_T Cuts at: 1203 2287 2433 3377 5152 6129 1203 Size: 1084 146 944 1775 977 1327 Fragments arranged by size: 1775 1327 1084 977 944 146 AgeI A'CCGG_T Cuts at: 5084 5084 Size: 6253 AhdI GACnn_n'nnGTC Cuts at: 4982 4982 Size: 6253 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6253 AlwNI CAG_nnn'CTG Cuts at: 2590 3521 3996 5568 2590 Size: 931 475 1572 3275 Fragments arranged by size: 3275 1572 931 475 ApaLI G'TGCA_C Cuts at: 3445 4310 5466 3445 Size: 865 1156 4232 Fragments arranged by size: 4232 1156 865 ApoI r'AATT_y Cuts at: 1493 2313 2861 2996 3562 3788 3940 6049 1493 Size: 820 548 135 566 226 152 2109 1697 Fragments arranged by size: 2109 1697 820 566 548 226 152 135 AseI AT'TA_AT Cuts at: 160 1032 2097 4807 160 Size: 872 1065 2710 1606 Fragments arranged by size: 2710 1606 1065 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 AvaI C'yCGr_G Cuts at: 1870 1943 3578 1870 Size: 73 1635 4545 Fragments arranged by size: 4545 1635 73 AvrII C'CTAG_G Cuts at: 3470 3470 Size: 6253 BamHI G'GATC_C Cuts at: 2545 3583 3950 2545 Size: 1038 367 4848 Fragments arranged by size: 4848 1038 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3574 5029 618 Size: 325 217 627 1787 1455 1842 Fragments arranged by size: 1842 1787 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2752 3277 3509 3572 1391 Size: 1361 525 232 63 4072 Fragments arranged by size: 4072 1361 525 232 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4444 4478 1903 Size: 34 50 34 2423 34 3678 Fragments arranged by size: 3678 2423 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4145 5355 4145 Size: 1210 5043 BclI T'GATC_A Cuts at: 1548 1548 Size: 6253 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4864 136 Size: 107 122 71 631 605 3192 1525 Fragments arranged by size: 3192 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2820 6248 2820 Size: 3428 2825 BlpI GC'TnA_GC Cuts at: 2977 3670 2977 Size: 693 5560 Bme1580I G_kGCm'C Cuts at: 1790 2799 3449 4314 5470 1790 Size: 1009 650 865 1156 2573 Fragments arranged by size: 2573 1156 1009 865 650 BmgBI CAC'GTC Cuts at: 1318 2434 1318 Size: 1116 5137 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3304 3984 4942 Size: 416 53 83 399 24 1887 680 958 Cuts at: 4942 442 Size: 1753 Fragments arranged by size: 1887 1753 958 680 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2561 4913 1239 Size: 824 498 2352 2579 Fragments arranged by size: 2579 2352 824 498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3128 4378 5243 5541 3128 Size: 1250 865 298 3840 Fragments arranged by size: 3840 1250 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4916 882 Size: 617 3417 2219 Fragments arranged by size: 3417 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4044 493 Size: 711 2840 2702 Fragments arranged by size: 2840 2702 711 BsaBI GATnn'nnATC Cuts at: 6247 6247 Size: 6253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4060 4442 5077 275 Size: 53 83 186 936 2527 382 635 1451 Fragments arranged by size: 2527 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4686 5084 5358 5505 5916 4686 Size: 398 274 147 411 5023 Fragments arranged by size: 5023 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6028 Fragments arranged by size: 6028 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2760 1186 Size: 1574 4679 BseYI C'CCAG_C Cuts at: 2102 3279 5456 5952 2102 Size: 1177 2177 496 2403 Fragments arranged by size: 2403 2177 1177 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 1163 Size: 573 840 4840 Fragments arranged by size: 4840 840 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3703 4464 5492 1540 Size: 275 186 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2366 2517 2752 3449 4314 4399 5470 2366 Size: 151 235 697 865 85 1071 3149 Fragments arranged by size: 3149 1071 865 697 235 151 85 BsmI GAATG_Cn' Cuts at: 3443 3764 3857 3443 Size: 321 93 5839 Fragments arranged by size: 5839 321 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2366 2517 2752 2799 3277 3449 3509 Size: 399 576 151 235 47 478 172 60 Cuts at: 3509 3572 4314 4399 5470 1391 Size: 63 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 576 478 399 235 172 151 85 63 60 47 BspEI T'CCGG_A Cuts at: 5916 5916 Size: 6253 BspHI T'CATG_A Cuts at: 4142 5872 4142 Size: 1730 4523 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2598 3341 3608 844 Size: 1754 743 267 3489 Fragments arranged by size: 3489 1754 743 267 BsrBI CCG'CTC Cuts at: 1394 1823 3091 4140 1394 Size: 429 1268 1049 3507 Fragments arranged by size: 3507 1268 1049 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4748 4922 65 Size: 1183 3500 174 1396 Fragments arranged by size: 3500 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4897 5084 1700 Size: 110 3087 187 2869 Fragments arranged by size: 3087 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6253 BssSI C'ACGA_G Cuts at: 4313 5325 4313 Size: 1012 5241 BstAPI GCAn_nnn'nTGC Cuts at: 794 3490 794 Size: 2696 3557 BstBI TT'CG_AA Cuts at: 2611 5057 2611 Size: 2446 3807 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6253 BstYI r'GATC_y Cuts at: 1972 2545 2556 2820 3583 3950 4335 4352 5804 Size: 573 11 264 763 367 385 17 1452 Cuts at: 5804 5890 5902 6248 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 763 573 385 367 346 264 86 17 12 11 BtgI C'CryG_G Cuts at: 513 1065 2799 513 Size: 552 1734 3967 Fragments arranged by size: 3967 1734 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5514 Fragments arranged by size: 5514 683 56 BtsI GCAGTG_nn' Cuts at: 2503 3769 4563 4583 2503 Size: 1266 794 20 4173 Fragments arranged by size: 4173 1266 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6253 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6218 DraI TTT'AAA Cuts at: 2079 2340 2406 3430 3557 2079 Size: 261 66 1024 127 4775 Fragments arranged by size: 4775 1024 261 127 66 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5260 809 Size: 507 3944 1802 Fragments arranged by size: 3944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2488 3458 3700 Size: 54 1425 50 275 18 658 970 242 Cuts at: 3700 4589 6218 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 970 889 658 275 242 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3700 1537 Size: 275 1888 4090 Fragments arranged by size: 4090 1888 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3044 3165 4183 1353 Size: 1691 121 1018 3423 Fragments arranged by size: 3423 1691 1018 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4840 5214 5360 387 Size: 1064 138 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2750 2750 Size: 6253 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2561 4316 4913 5700 5968 1239 Size: 393 431 498 1755 597 787 268 1524 Fragments arranged by size: 1755 1524 787 597 498 431 393 268 EcoO109I rG'GnC_Cy Cuts at: 3410 3518 3410 Size: 108 6145 EcoRI G'AATT_C Cuts at: 3562 3940 3562 Size: 378 5875 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2432 2702 2432 Size: 270 5983 FspI TGC'GCA Cuts at: 4759 4759 Size: 6253 HaeII r_GCGC'y Cuts at: 1678 5400 1678 Size: 3722 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3236 3268 4894 4926 4968 Size: 32 135 32 1664 32 1626 32 42 Cuts at: 4968 5000 1373 Size: 32 2626 Fragments arranged by size: 2626 1664 1626 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3022 3597 3843 669 Size: 988 281 1084 575 246 3079 Fragments arranged by size: 3079 1084 988 575 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6253 HpaI GTT'AAC Cuts at: 3843 3843 Size: 6253 KpnI G_GTAC'C Cuts at: 3578 3578 Size: 6253 MfeI C'AATT_G Cuts at: 3852 3852 Size: 6253 MluI A'CGCG_T Cuts at: 6129 6129 Size: 6253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2228 5367 5551 1305 Size: 923 3139 184 2007 Fragments arranged by size: 3139 2007 923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2490 10 Size: 54 1425 343 658 3773 Fragments arranged by size: 3773 1425 658 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2307 2427 4211 4570 4729 518 Size: 648 1120 21 120 1784 359 159 2042 Fragments arranged by size: 2042 1784 1120 648 359 159 120 21 MspA1I CmG'CkG Cuts at: 1606 3084 3279 3669 4346 5494 5739 1606 Size: 1478 195 390 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 390 245 195 NcoI C'CATG_G Cuts at: 513 1065 2799 513 Size: 552 1734 3967 Fragments arranged by size: 3967 1734 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6253 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6253 NotI GC'GGCC_GC Cuts at: 3700 3700 Size: 6253 NspI r_CATG'y Cuts at: 1132 1229 2291 2306 3381 3491 3611 5156 1132 Size: 97 1062 15 1075 110 120 1545 2229 Fragments arranged by size: 2229 1545 1075 1062 120 110 97 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6253 PciI A'CATG_T Cuts at: 2287 3377 5152 2287 Size: 1090 1775 3388 Fragments arranged by size: 3388 1775 1090 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 1108 Size: 1431 4822 PmeI GTTT'AAAC Cuts at: 3557 3557 Size: 6253 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4322 4354 5835 5867 4322 Size: 32 1481 32 4708 Fragments arranged by size: 4708 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6253 PsiI TTA'TAA Cuts at: 3823 3823 Size: 6253 PstI C_TGCA'G Cuts at: 830 2389 2623 3605 830 Size: 1559 234 982 3478 Fragments arranged by size: 3478 1559 982 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6253 SacI G_AGCT'C Cuts at: 2752 2752 Size: 6253 SalI G'TCGA_C Cuts at: 1936 3595 1936 Size: 1659 4594 SbfI CC_TGCA'GG Cuts at: 3605 3605 Size: 6253 ScaI AGT'ACT Cuts at: 4501 6213 4501 Size: 1712 4541 SfcI C'TryA_G Cuts at: 826 1045 2385 2619 3601 4736 5417 5608 826 Size: 219 1340 234 982 1135 681 191 1471 Fragments arranged by size: 1471 1340 1135 982 681 234 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6253 SmaI CCC'GGG Cuts at: 1872 3580 1872 Size: 1708 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3143 4357 5258 5520 820 Size: 197 926 1200 1214 901 262 1553 Fragments arranged by size: 1553 1214 1200 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4044 493 Size: 3551 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6253 SphI G_CATG'C Cuts at: 1132 3611 1132 Size: 2479 3774 SspI AAT'ATT Cuts at: 5 52 4177 5 Size: 47 4125 2081 Fragments arranged by size: 4125 2081 47 StuI AGG'CCT Cuts at: 1517 2171 1517 Size: 654 5599 StyI C'CwwG_G Cuts at: 513 1065 2029 2799 2968 3114 3470 3692 513 Size: 552 964 770 169 146 356 222 3074 Fragments arranged by size: 3074 964 770 552 356 222 169 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4280 4297 4450 4635 5080 325 Size: 1255 2700 17 153 185 445 1498 Fragments arranged by size: 2700 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2135 3063 4499 Size: 274 80 33 51 1443 158 928 1436 Cuts at: 4499 6211 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1436 928 274 158 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2629 2661 2699 2731 3428 3460 6213 6245 2629 Size: 32 38 32 697 32 2753 32 2637 Fragments arranged by size: 2753 2637 697 38 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6253 XbaI T'CTAG_A Cuts at: 3031 3589 3031 Size: 558 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2671 3146 2671 Size: 475 5778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6253 XmaI C'CCGG_G Cuts at: 1870 3578 1870 Size: 1708 4545 XmnI GAAnn'nnTTC Cuts at: 3174 4382 3174 Size: 1208 5045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4061 276 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI