(Circular) (Six-base) MAPSORT of: pFN21AB4038.seq Check: 4829 from: 1 to: 5794 pFN21AB4038 5794 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2634 3137 1937 Size: 697 503 4594 Fragments arranged by size: 4594 697 503 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3857 5241 5509 1632 Size: 2225 1384 268 1917 Fragments arranged by size: 2225 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2035 1184 Size: 851 4943 AlwNI CAG_nnn'CTG Cuts at: 2887 3537 5109 2887 Size: 650 1572 3572 Fragments arranged by size: 3572 1572 650 ApaI G_GGCC'C Cuts at: 2250 2250 Size: 5794 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 2209 3103 3329 3481 5590 1493 Size: 716 894 226 152 2109 1697 Fragments arranged by size: 2109 1697 894 716 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 3119 1870 Size: 73 1176 4545 Fragments arranged by size: 4545 1176 73 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2897 3115 4570 618 Size: 325 217 627 1110 218 1455 1842 Fragments arranged by size: 1842 1455 1110 627 325 218 217 BanII G_rGCy'C Cuts at: 1391 2161 2250 2307 3113 1391 Size: 770 89 57 806 4072 Fragments arranged by size: 4072 806 770 89 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2910 3076 928 Size: 971 1011 166 3646 Fragments arranged by size: 3646 1011 971 166 BbvCI CC'TCA_GC Cuts at: 2664 2664 Size: 5794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3985 4019 1903 Size: 34 2048 34 3678 Fragments arranged by size: 3678 2048 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4405 136 Size: 107 122 71 631 605 337 2396 1525 Fragments arranged by size: 2396 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2784 2955 5789 2784 Size: 171 2834 2789 Fragments arranged by size: 2834 2789 171 BlpI GC'TnA_GC Cuts at: 3211 3211 Size: 5794 Bme1580I G_kGCm'C Cuts at: 1790 2250 3855 5011 1790 Size: 460 1605 1156 2573 Fragments arranged by size: 2573 1605 1156 460 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2671 3525 4483 Size: 416 53 83 399 24 1254 854 958 Cuts at: 4483 442 Size: 1753 Fragments arranged by size: 1753 1254 958 854 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2321 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2488 2660 2765 2864 3011 4454 1239 Size: 1249 172 105 99 147 1443 2579 Fragments arranged by size: 2579 1443 1249 172 147 105 99 Bpu10I CC'TnA_GC Cuts at: 2472 2664 2472 Size: 192 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2350 3919 4784 5082 2350 Size: 1569 865 298 3062 Fragments arranged by size: 3062 1569 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2666 4457 882 Size: 617 1167 1791 2219 Fragments arranged by size: 2219 1791 1167 617 BsaAI yAC'GTr Cuts at: 493 1204 3585 493 Size: 711 2381 2702 Fragments arranged by size: 2702 2381 711 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3601 3983 4618 275 Size: 53 83 186 936 2068 382 635 1451 Fragments arranged by size: 2068 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3087 4227 4625 4899 5046 5457 3087 Size: 1140 398 274 147 411 3424 Fragments arranged by size: 3424 1140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2517 2547 2933 2963 1178 Size: 30 165 30 1114 30 386 30 4009 Fragments arranged by size: 4009 1114 386 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2315 2954 1186 Size: 880 249 639 4026 Fragments arranged by size: 4026 880 639 249 BseYI C'CCAG_C Cuts at: 2493 2569 4997 5493 2493 Size: 76 2428 496 2794 Fragments arranged by size: 2794 2428 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2532 1163 Size: 573 796 4425 Fragments arranged by size: 4425 796 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3244 4005 5033 1540 Size: 275 186 1243 761 1028 2301 Fragments arranged by size: 2301 1243 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2397 3855 3940 5011 2307 Size: 90 1458 85 1071 3090 Fragments arranged by size: 3090 1458 1071 90 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2250 2307 2397 3113 3855 3940 Size: 399 371 89 57 90 716 742 85 Cuts at: 3940 5011 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 716 399 371 90 89 85 57 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 2307 3683 5413 2307 Size: 1376 1730 2688 Fragments arranged by size: 2688 1730 1376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2024 2198 2668 3149 844 Size: 1180 174 470 481 3489 Fragments arranged by size: 3489 1180 481 470 174 BsrBI CCG'CTC Cuts at: 1394 1823 3681 1394 Size: 429 1858 3507 Fragments arranged by size: 3507 1858 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4289 4463 65 Size: 1183 3041 174 1396 Fragments arranged by size: 3041 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3054 4438 4625 1700 Size: 110 1244 1384 187 2869 Fragments arranged by size: 2869 1384 1244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5794 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 2442 3854 4866 2442 Size: 1412 1012 3370 Fragments arranged by size: 3370 1412 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5794 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 3007 1184 Size: 1823 3971 BstYI r'GATC_y Cuts at: 1972 2784 2955 3124 3491 3876 3893 5345 5431 Size: 812 171 169 367 385 17 1452 86 Cuts at: 5431 5443 5789 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 812 385 367 346 171 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2049 2049 Size: 5794 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5242 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 3310 4104 4124 3310 Size: 794 20 4980 Fragments arranged by size: 4980 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2134 2169 529 Size: 35 1570 35 4154 Fragments arranged by size: 4154 1570 35 35 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2702 4801 809 Size: 507 1386 2099 1802 Fragments arranged by size: 2099 1802 1386 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2407 2602 Size: 54 1425 50 275 18 345 232 195 Cuts at: 2602 2649 2998 3241 4130 5759 8 Size: 47 349 243 889 1629 43 Fragments arranged by size: 1629 1425 889 349 345 275 243 232 195 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3241 1537 Size: 275 1429 4090 Fragments arranged by size: 4090 1429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2545 3724 1353 Size: 1026 166 1179 3423 Fragments arranged by size: 3423 1179 1026 166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4381 4755 4901 387 Size: 1064 138 2792 374 146 1280 Fragments arranged by size: 2792 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2305 2305 Size: 5794 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2488 2660 2765 2864 3011 3857 4454 Size: 393 856 172 105 99 147 846 597 Cuts at: 4454 5241 5509 1239 Size: 787 268 1524 Fragments arranged by size: 1524 856 846 787 597 393 268 172 147 105 99 EcoO109I rG'GnC_Cy Cuts at: 2056 2247 2718 2056 Size: 191 471 5132 Fragments arranged by size: 5132 471 191 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 4941 1678 Size: 3263 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2323 2355 2482 2514 2663 Size: 32 135 32 751 32 127 32 149 Cuts at: 2663 2695 2776 2808 4435 4467 4509 4541 1373 Size: 32 81 32 1627 32 42 32 2626 Fragments arranged by size: 2626 1627 751 149 135 127 81 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2806 3138 3384 669 Size: 988 281 868 332 246 3079 Fragments arranged by size: 3079 988 868 332 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 4908 5092 1305 Size: 813 2790 184 2007 Fragments arranged by size: 2790 2007 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2409 2604 2651 3000 10 Size: 54 1425 343 577 195 47 349 2804 Fragments arranged by size: 2804 1425 577 349 343 195 54 47 MslI CAynn'nnrTG Cuts at: 518 1166 2312 3752 4111 4270 518 Size: 648 1146 1440 359 159 2042 Fragments arranged by size: 2042 1440 1146 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2776 3210 3887 5035 5280 1606 Size: 1170 434 677 1148 245 2120 Fragments arranged by size: 2120 1170 1148 677 434 245 NaeI GCC'GGC Cuts at: 3056 3056 Size: 5794 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5242 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NgoMIV G'CCGG_C Cuts at: 3054 3054 Size: 5794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 3083 3152 4697 1132 Size: 97 1854 69 1545 2229 Fragments arranged by size: 2229 1854 1545 97 69 PasI CC'CwG_GG Cuts at: 1348 2068 2099 1348 Size: 720 31 5043 Fragments arranged by size: 5043 720 31 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 2887 1108 Size: 1779 4015 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3863 3895 5376 5408 3863 Size: 32 1481 32 4249 Fragments arranged by size: 4249 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2056 Size: 5794 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 2246 2246 Size: 5794 PstI C_TGCA'G Cuts at: 830 3146 830 Size: 2316 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 PvuII CAG'CTG Cuts at: 2776 2776 Size: 5794 RsrII CG'GwC_CG Cuts at: 3090 3090 Size: 5794 SacI G_AGCT'C Cuts at: 2307 2307 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SapI GCTCTTCn'nnn_ Cuts at: 2379 2379 Size: 5794 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SexAI A'CCwGG_T Cuts at: 2886 2886 Size: 5794 SfcI C'TryA_G Cuts at: 826 1045 2283 3142 4277 4958 5149 826 Size: 219 1238 859 1135 681 191 1471 Fragments arranged by size: 1471 1238 1135 859 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 3121 1872 Size: 1249 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2329 3898 4799 5061 820 Size: 197 926 386 1569 901 262 1553 Fragments arranged by size: 1569 1553 926 901 386 262 197 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 3152 1132 Size: 2020 3774 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 2087 2483 2760 1517 Size: 570 396 277 4551 Fragments arranged by size: 4551 570 396 277 StyI C'CwwG_G Cuts at: 513 1065 2404 3233 513 Size: 552 1339 829 3074 Fragments arranged by size: 3074 1339 829 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2542 2986 3821 3838 3991 4176 4621 Size: 1255 962 444 835 17 153 185 445 Cuts at: 4621 325 Size: 1498 Fragments arranged by size: 1498 1255 962 835 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4040 5752 96 Size: 274 80 33 51 1443 2063 1712 138 Fragments arranged by size: 2063 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2517 2548 2549 2580 2933 2965 5754 5786 2517 Size: 31 1 31 353 32 2789 32 2525 Fragments arranged by size: 2789 2525 353 32 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 1316 2677 1316 Size: 1361 4433 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2240 2240 Size: 5794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 3119 1870 Size: 1249 4545 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI SacII SanDI SfiI SfoI SrfI SwaI