Restriction map for
pFN21AB4028
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AflIII AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbvCI BclI BfrI
BglI BglII BlnI BmyI BsaAI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsoBI Bsp1286I Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MluI MluNI MroI MscI NaeI
NarI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 734 AACGTT AA^CGTT
AfaI 835 GTAC GT^AC
AgeI 143 ACCGGT A^CCGGT
AocI 702 CCTCAGG CC^T[ACGT]AGG
AvaII 82 GGTCC G^G[AT]CC
BamHI 707 GGATCC G^GATCC
BbsI 871 GAAGAC GAAGAC(2/6)
BcgI 177 GCAGCCAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1584 CCGGG CC^[GC]GG
BlpI 1596 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1596 GCTGAGC GC^T[ACGT]AGC
BpuAI 871 GAAGAC GAAGAC(2/6)
BsaBI 1360 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 143 ACCGGT [AT]^CCGG[AT]
BsgI 634 CTGCAC GTGCAG(16/14)
BsmBI 1348 GAGACG CGTCTC(1/5)
BsmFI 1252 GTCCC GGGAC(10/14)
BspDI 1268 ATCGAT AT^CGAT
BsrFI 143 ACCGGT [GA]^CCGG[CT]
BstYI 707 GGATCC [GA]^GATC[CT]
Bsu36I 702 CCTCAGG CC^T[ACGT]AGG
CelII 1596 GCTGAGC GC^T[ACGT]AGC
Cfr10I 143 ACCGGT [GA]^CCGG[CT]
Cfr13I 82 GGTCC G^G[ACGT]CC
ClaI 1268 ATCGAT AT^CGAT
Eco81I 702 CCTCAGG CC^T[ACGT]AGG
EcoT22I 89 ATGCAT ATGCA^T
HaeII 15 GGCGCT [GA]GCGC^[CT]
HaeIII 1582 GGCC GG^CC
HincII 157 GTTGAC GT[CT]^[GA]AC
HindII 157 GTTGAC GT[CT]^[GA]AC
MamI 1360 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 860 CAATTG C^AATTG
MflI 707 GGATCC [GA]^GATC[CT]
MslI 307 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 860 CAATTG C^AATTG
NciI 1584 CCGGG CC^[GC]GG
NcoI 1233 CCATGG C^CATGG
NdeI 416 CATATG CA^TATG
NsiI 89 ATGCAT ATGCA^T
PflMI 1315 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 143 ACCGGT A^CCGGT
Psp1406I 734 AACGTT AA^CGTT
RsaI 835 GTAC GT^AC
Sau96I 82 GGTCC G^G[ACGT]CC
Van91I 1315 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1260 TCTAGA T^CTAGA
XhoII 707 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 510 CGCG CG^CG
1210 CGCG
AlwNI 49 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
964 CAGGCTCTG
BfaI 896 CTAG C^TAG
1261 CTAG
BseRI 1159 GAGGAG GAGGAG(10/8)
1162 GAGGAG
BsmAI 1348 GAGAC GTCTC(1/5)
1290 GTCTC
BsrDI 442 GCAATG GCAATG(2/0)
1486 GCAATG
BstUI 510 CGCG CG^CG
1210 CGCG
CfoI 16 GCGC GCG^C
1211 GCGC
DsaI 593 CCGTGG C^C[GA][CT]GG
1233 CCATGG
EcoRI 1310 GAATTC G^AATTC
1434 GAATTC
EcoT14I 989 CCTTGG C^C[AT][AT]GG
1233 CCATGG
FauI 162 CCCGC CCCGC(4/6)
1646 CCCGC
HapII 144 CCGG C^CGG
1584 CCGG
HhaI 16 GCGC GCG^C
1211 GCGC
HinP1I 16 GCGC G^CGC
1211 GCGC
HpaII 144 CCGG C^CGG
1584 CCGG
MaeI 896 CTAG C^TAG
1261 CTAG
MspI 144 CCGG C^CGG
1584 CCGG
MvnI 510 CGCG CG^CG
1210 CGCG
NlaIII 867 CATG CATG^
1234 CATG
PvuII 817 CAGCTG CAG^CTG
1670 CAGCTG
StyI 989 CCTTGG C^C[AT][AT]GG
1233 CCATGG
TfiI 1360 GATTC G^A[AT]TC
1383 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I BbvI BciVI BpmI BsaJI
BsiYI BslI BsmI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
DraI EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIV PleI Sau3AI
ScrFI SfaNI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI